土壤源孔雀石绿脱色菌的筛选及脱色条件分析

doi:10.14088/j.cnki.issn0439-8114.2020.22.015

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (22): 86-90.doi: 10.14088/j.cnki.issn0439-8114.2020.22.015

土壤源孔雀石绿脱色菌的筛选及脱色条件分析

廖华媛, 熊梦霞, 李润成

湖南农业大学动物医学院,长沙 410128

收稿日期:2020-02-20 出版日期:2020-11-25 发布日期:2020-12-30
通讯作者: 李润成(1979-),男,实验师,博士,主要从事动物疫病防控研究,(电子信箱)961095695@qq.com
作者简介:廖华媛(1994-),女,湖南郴州人,在读硕士研究生,研究方向为动物微生物与免疫,(电话)13548971153(电子信箱)1325288221@qq.com
基金资助:
国家重点研发项目(2017YFD0500102)

Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis

LIAO Hua-yuan, XIONG Meng-xia, LI Run-cheng

College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China

Received:2020-02-20 Online:2020-11-25 Published:2020-12-30

摘要/Abstract

摘要： 从土壤来源的细菌中,筛选可使孔雀石绿(MG)脱色的菌株,结果得到1株阴沟肠杆菌(Enterobacter cloacae)(S1-2)对MG有较强的脱色能力。通过MG被脱色前后溶液吸光度的变化衡量脱色率,用单一变量法分析影响菌株S1-2发挥MG脱色作用的不同条件。结果表明,菌株S1-2在36 h内可使1100 mg/L的MG完全脱色,处理后的MG对解淀粉芽孢杆菌、壁芽孢杆菌抑制率降低了43.98%~48.21%。用300 mg/L的MG进行脱色条件分析发现,按0.5%~7.0%接菌,脱色率达91.67%~93.91%;不同pH培养环境中,该菌在pH6、7时对MG脱色率为90.02%~94.54%;在20~40 ℃条件下培养,该菌对MG脱色率达84.61%~94.56%;在6~18 g/L的NaCl浓度下,该菌对MG的脱色率达91.85%~93.77%。

关键词: 孔雀石绿, 阴沟肠杆菌(Enterobacter cloacae), 毒性测试, 脱色条件

Abstract: To screen bacteria capable of decolorizing malachite green (MG) from the soil-derived bacteria,as a result,a strain of Enterobacter cloacae (S1-2) had a strong ability to decolorize MG.According to single variable principle,the different conditions that affected the strain S1-2 were characterized by the change in absorbance of the solution before and after MG degradation.The results showed that the strain S1-2 could completely decolorize1100 mg/L MG within36 h,and the inhibitory rate of treated MG on Bacillus amyloliquefaciens and Bacillus parvii decreased by43.98%~48.21%.The analysis of decolorization conditions with300 mg/L MG found that the degradation rate of 0.5%~7.0% inoculation amount of strain S1-2 was91.67%~93.91%;In different pH culture environment,the strain S1-2 decolorized90.02%~94.54% MG at pH6 and pH7;The strain S1-2 decomposed84.61%~94.56% MG,when cultured at20~40 ℃;Under the concentration of6~18 g/L NaCl,the strain S1-2 degraded91.85%~93.77% MG.

Key words: malachite green, Enterobacter cloacae, toxicity test, decolorization characteristics

中图分类号:

X172
X788

廖华媛, 熊梦霞, 李润成. 土壤源孔雀石绿脱色菌的筛选及脱色条件分析[J]. 湖北农业科学, 2020, 59(22): 86-90.

LIAO Hua-yuan, XIONG Meng-xia, LI Run-cheng. Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 86-90.

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土壤源孔雀石绿脱色菌的筛选及脱色条件分析

Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis

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