湖北农业科学 ›› 2020, Vol. 59 ›› Issue (23): 24-27.doi: 10.14088/j.cnki.issn0439-8114.2020.23.005

• 育种·栽培 • 上一篇    下一篇

分子标记辅助选择培育抗稻瘟病和抗褐飞虱多基因聚合的水稻恢复系

李进波, 杜雪树, 夏明元, 万丙良, 戚华雄   

  1. 湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉 430064
  • 收稿日期:2020-04-15 出版日期:2020-12-10 发布日期:2020-12-30
  • 通讯作者: 戚华雄,研究员,主要从事水稻育种研究工作,(电话)027-87389224(电子信箱)qi_huaxiong@163.com
  • 作者简介:李进波(1971-),男,湖北天门人,副研究员,主要从事水稻育种研究工作,(电话)027-87389224(电子信箱)jinboli0606@163.com
  • 基金资助:
    国家水稻产业技术体系武汉综合试验站项目(CARS-01-78);湖北省农业科技创新中心资助项目(2016-620-000-001-003)

Developing restorer line pyramiding different resistant genes to blast and brown planthopper by molecular marker-assisted selection in rice

LI Jin-bo, DU Xue-shu, XIA Ming-yuan, WAN Bing-liang, QI Hua-xiong   

  1. Food Crops Institute,Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Wuhan 430064,China
  • Received:2020-04-15 Online:2020-12-10 Published:2020-12-30

摘要: 以R022为轮回亲本、GD7为供体亲本,利用分子标记辅助选择技术培育出聚合有Pi1、Pi2、Bph14Bph15基因的恢复系R650,抗性鉴定结果表明,R650对稻瘟病和褐飞虱的抗性均表现为抗。R650与不育系配制的杂交组合巨风2A/R650、天丰A/R650和内香5A/R650对稻瘟病的抗性表现为中抗或中感,对褐飞虱的抗性表现为中抗,且配制的这3个杂交组合的产量高于或相当于轮回亲本R022配制的杂交组合巨风2A/R022、天丰A/R022和内香5A/R022,其中巨风优650(巨风2A/R650)于2019年通过湖北省农作物品种审定委员会审定。

关键词: 水稻(Oryza sativa L.), 稻瘟病, 褐飞虱, 分子标记辅助选择

Abstract: R022 was used as the receptor parent and GD7 was used as the donor parent to pyramid 4 resistance genes Pi1,Pi2,Bph14 and Bph15 into R650 by molecular marker-assisted selection (MAS).Resistance identification demonstrated that R650 performed resistance to rice blast and brown planthopper.Jufeng 2A/R650,Tianfeng A/R650 and Neixiang 5A/R50,the three hybrid combinations with R650 as restorer line and three sterile lines,performed moderately resistance or moderately susceptible to rice blast and moderately resistance to brown planthopper.Field trial data demonstrated that yields of these three hybrid combinations were higher than or equal to the control hybrid combinations of Jufeng 2A/R022,Tianfeng A/R022,Neixiang 5A/R022 with R022 as recurrent parent under natural field conditions.The combination Jufengyou 650 (Jufeng 2A/R650) was authorized by Hubei Crop Variety Examination and Approving Committee in 2019.

Key words: rice(Oryza sativa L.), blast, brown planthopper, molecular marker-assisted selection (MAS)

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