广西斑地锦及其混淆品DNA条形码分子鉴定

doi:10.14088/j.cnki.issn0439-8114.2022.10.028

湖北农业科学 ›› 2022, Vol. 61 ›› Issue (10): 156-159.doi: 10.14088/j.cnki.issn0439-8114.2022.10.028

广西斑地锦及其混淆品DNA条形码分子鉴定

侯静, 黄勇, 黎理, 王素娥, 蔡毅

广西中医药大学,南宁 530001

收稿日期:2021-06-07 出版日期:2022-05-25 发布日期:2022-06-14
通讯作者: 蔡毅（1962-）,男,广西南宁人,教授,主要从事中药鉴定学研究工作,（电子信箱）Caiyi118@163.com。
作者简介:侯静（1995-）,女,广西桂平人,在读硕士研究生,研究方向为中药鉴定学,（电话）18878793603（电子信箱）840184951@qq.com。
基金资助:
广西自然科学基金项目（2017GXNSFDA198001）; 广西壮瑶药重点实验室项目（桂科基字【2014】32号）; 广西中医药大学校级课题（H2014015）

Identification of Euphorbia maculata L. and its adulterants in Guangxi based on DNA barcode technology

HOU Jing, HUANG Yong, LI Li, WANG Su-e, CAI Yi

Guangxi University of Chinese Medicine, Nanning 530001,China

Received:2021-06-07 Online:2022-05-25 Published:2022-06-14

摘要/Abstract

摘要： 为鉴定斑地锦（Euphorbia maculata L.）及其混淆品,以广西采集的41份斑地锦及其混淆品为研究对象,提取其基因组DNA,对各个样品的条形码rbcL和psbA-trnH序列进行扩增并测序,与GenBank下载的13份斑地锦及其混淆品rbcL和psbA-trnH序列进行了分析比对,计算了斑地锦及其混淆品的种内、种间遗传距离并采用邻接法构建了系统发育树。结果发现,斑地锦种内遗传距离明显小于其与混淆品的遗传距离,系统发育树中斑地锦样品聚为单系并能够与其混淆品明显区分开,这表明rbcL和psbA-trnH序列可用于斑地锦及其常见的混淆品的鉴别。

关键词: 斑地锦（Euphorbia maculata L.）, 混淆品, DNA条形码, 遗传距离, 系统发育树

Abstract: In order to identify the species of Euphorbia maculata L. and its adulterants,the total genomic DNAs were extracted from 41 samples of Euphorbia maculata L. and its adulterants from Guangxi, and the barcode rbcL and psbA-trnH sequences of each sample were amplified and sequenced, and compared with the rbcL and psbA-trnH sequences of 13 samples downloaded by GenBank. The intraspecific and interspecific genetic distances of Euphorbia maculata L. and its adulterants were calculated and the phylogenetic tree was constructed by the neighbor-joining method. The results showed that the intraspecific genetic distance was significantly smaller than the interspecific genetic distance between Euphorbia maculata L. and its adulterants. The phylogenetic tree analysis results showed that the samples of Euphorbia maculata L. were clustered into a single line,which indicated that rbcL and psbA-trnH sequences could distinguish Euphorbia maculata L. and its adulterants.

Key words: Euphorbia maculata L., adulterants, DNA barcode, genetic distance, phylogenetic tree

中图分类号:

S567.219

侯静, 黄勇, 黎理, 王素娥, 蔡毅. 广西斑地锦及其混淆品DNA条形码分子鉴定[J]. 湖北农业科学, 2022, 61(10): 156-159.

HOU Jing, HUANG Yong, LI Li, WANG Su-e, CAI Yi. Identification of Euphorbia maculata L. and its adulterants in Guangxi based on DNA barcode technology[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(10): 156-159.

参考文献

[1] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2020.
[2] 刘静. 斑地锦正丁醇萃取物对小鼠肝损伤的保护作用及其化学成分研究[D].武汉:湖北中医药大学,2017.
[3] 王晓敏,高增光,邹志坚.地锦草对移植性肝癌H22小鼠抑瘤率和免疫功能的影响[J].中国老年学杂志,2015,35(13):3505-3507.
[4] 王培军. 地锦草黄酮醇抗肿瘤作用及其机理的研究[D].河北秦皇岛:燕山大学,2013.
[5] AHN S, JUNG H, JUNG Y, et al.Identification of the active components inhibiting the expression of matrix metallopeptidase-9 by TNFα in ethyl acetate extract of Euphorbia humifusa Willd[J]. Journal of applied biological chemistry,2019, 62(4):367-374.
[6] 王耀杰,相晓晗,韩丽娜,等.中药地锦草乙醇提取物体外抑制胃癌细胞增殖活性及机制研究[J].癌变·畸变·突变,2020,32(3):187-193.
[7] 刘丽萍,王邦才.运用资生洁肠饮治疗肠癌的经验[J].浙江中医杂志,2019,54(10):708.
[8] 胡建新,席晓甜,王晓敏,等.地锦草调控NF-κB/VEGF信号通路抑制肿瘤血管生成的机制[J].中国实验方剂学杂志,2018,24(23):165-170.
[9] 江涛,周兵,付丹,等.地锦草对Hela细胞增殖与凋亡的影响[J].中国现代中药,2017,19(12):1713-1716,1727.
[10] 邹志坚,刘海云,高增光,等.地锦草水提液对H22荷瘤小鼠生长抑制及其机制探讨[J].中华肿瘤防治杂志,2014,21(12):903-908.
[11] 王琳琳,富宏,黎巍威,等.地锦草改善2型糖尿病模型KK-Ay小鼠胰岛素抵抗的机制研究[J].中国中药杂志,2015,40(10):1994-1998.
[12] 周志愉,谢斌,王晓敏.地锦草水提液对糖尿病小鼠的降血糖作用[J].南昌大学学报(医学版),2014,54(6):5-7,11.
[13] RHIM T J.Comparison of radical scavenging, anticytotoxic, and anti-inflammatory effects of Euphorbia maculata and E. supina[J]. Journal of environmental science international, 2016, 25(8):1131-1142.
[14] KWON S, CHA J, LEE H, et al.Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation[J]. Molecular medicine reports,2015,11(6):4255-4261.
[15] 马金双,程用谦.中国植物志[M].北京:科学出版社,1997.47-53.
[16] 何报作,刘寿养,廖月葵,等.3种大戟科植物广西新记录及其同2种易混淆中药的区别特征[J].广西科学,2008(3):309-311,316.
[17] 蔡毅,侯静,黎理,等.广西大戟科新记录——台西地锦[J].浙江林业科技,2020,40(4):60-62.
[18] ALZOHAIRY A M.BioEdit: An important software for molecular biology[J].Gerf bulletin of biosciences, 2011, 2(1):60.
[19] KANG L,XIE D F, XIAO Q Y, et al.Sequencing and analyses on chloroplast genomes of Tetrataenium candicans and two allies give new insights on structural variants, DNA barcoding and phylogeny in Apiaceae subfamily Apioideae[J]. PeerJ, 2019,7:e8063.
[20] KHAN S A, BAESHEN M N, RAMADAN H A, et al.ITS2: An ideal DNA barcode for the arid medicinal plant rhazya stricta[J].Pharmaceut Med, 2019,33(1):53-61.
[21] SHI R, HU Z, LU H, et al.Hierarchical nanostructuring array enhances mid-hybridization for accurate herbal identification via ITS2 DNA barcode[J].Anal Chem, 2020,92(2):2136-2144.
[22] INTHARUKSA A, SASAKI Y, ANDO H, et al.The combination of ITS2 and psbA-trnH region is powerful DNA barcode markers for authentication of medicinal terminalia plants from Thailand[J].J Nat Med, 2020,74(1):282-293.
[23] FENG S G, JIAO K L, ZHU Y J, et al.Molecular identification of species of physalis (solanaceae) using a candidate DNA barcode: The chloroplast psbA-trnH intergenic region[J].Genome, 2018,61(1):15-20.
[24] JIN W T, SCHUITEMAN A, CHASE M W, et al.Phylogenetics of subtribe Orchidinae s.L. (Orchidaceae; Orchidoideae) based on seven markers(plastid matK,psaB, rbcl, trnl-F, trnH-psba, and nuclear nrITS, Xdh):Implications for generic delimitation[J].BMC Plant Biol, 2017,17(1):222-228.
[25] KRESS W J,ERICKSON D L,JONES F A,et al.Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama[J].Proc Natl Acad Sci,2009,106(44):18621-18626.
[26] SWENSON N G,ANGLADA-CORDERO P,BARONE J A.Deterministic tropical tree community turnover: Evidence from patterns of functional beta diversity along an elevational gradient[J].Proc Biol Sci,2011,278(1707) : 877-884.
[27] CHENG F T,LI Z H,LIU C Y,et al.DNA Barcoding of the genus Rehmannia (Scrophulariaceae)[J].Plant Sci J,2015,33(1):25-32.

广西斑地锦及其混淆品DNA条形码分子鉴定

Identification of Euphorbia maculata L. and its adulterants in Guangxi based on DNA barcode technology

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