关键词: 甘蓝（Brassica oleracea var. capitata）, 流式细胞仪, 花粉, 测定, 倍性, 裂解液

Abstract: Pollen ploidy of Brassica oleracea var. capitata was determined by flow cytometry. Pollen of cabbage GL21028 was used as the material, and leaves were used as the control to compare the effects of two pollen treatments, three different cell lysates, and two nucleic acid dyes. Pollen cells obtained through B buffer could be directly stained, which was the easiest treatment method. Cabbage leaves and pollen were treated with “Aru” buffer and Y lysates and stains DAPI, and flow cytometry was used for detection. The DNA of the sample cells was concentrated, and there were few cell fragments. The prepared Y lysate could be used for the observation of cabbage material by flow cytometry. Cabbage pollen treated with B buffer could be directly used as the material for flow cytometry ploidy identification, no other treatment was required, and the prepared Y lysate could obtain better test results.

Key words: Brassica oleracea var. capitata, flow cytometry, pollen, determination, ploidy, lysate