湖北农业科学 ›› 2025, Vol. 64 ›› Issue (10): 153-157.doi: 10.14088/j.cnki.issn0439-8114.2025.10.023

• 水产养殖 • 上一篇    下一篇

蚓粪浸出液-BG11培养基配比差异对蛋白核小球藻种群增长的影响

郑睿涵, 马若冰, 解子杨, 李俐琼   

  1. 中国农业大学烟台研究院,山东 烟台 264670
  • 收稿日期:2025-06-04 出版日期:2025-10-25 发布日期:2025-11-14
  • 通讯作者: 李俐琼(1981-),女,湖北郧西人,博士,副教授,主要从事生物饵料培养学研究,(电话)15288792237(电子信箱)lilyjone_new@163.com。
  • 作者简介:郑睿涵(2004-),女,山东济南人,本科生,专业方向为水产养殖,(电话)13805417606(电子信箱)13805417606@163.com。
  • 基金资助:
    烟台市2022年校地融合项目; 烟台市科技计划项目(2022XDRH025); 中国农业大学烟台研究院本科生URP项目(U2024011)

Effects of ratio variations of earthworm feces extract-BG11 medium on the population growth of Chlorella pyrenoidosa

ZHENG Rui-han, MA Ruo-bing, XIE Zi-yang, LI Li-qiong   

  1. Yantai Institute, China Agricultural University, Yantai 264670, Shandong, China
  • Received:2025-06-04 Published:2025-10-25 Online:2025-11-14

摘要: 为探索蚯蚓粪便在微藻培养上的利用价值,采用梯度稀释法设计11种蚓粪浸出液与BG11培养基以及去离子水的复合培养体系,分析不同配比对蛋白核小球藻(Chlorella pyrenoidosa)生长动力学的影响。结果表明,C3(V蚓粪浸出液V=5∶5)在多维评价体系中表现出最优的产业化适配性,在单位生产成本较BG11对照组降低91.40%的同时,仍能表现出较高的生产效能,生物量达到(1.340 ± 0.071)×107个/mL,相比生长率较BG11对照组提升48.77%,衰减动力学参数降低84.67%,为蛋白核小球藻的低成本生产提供了创新解决方案。

关键词: 蚓粪浸出液, 蛋白核小球藻(Chlorella pyrenoidosa), BG11, 种群增长

Abstract: To investigate the utilization potential of earthworm feces extract in microalgal cultivation, this study constructed composite culture systems incorporating 11 gradient-diluted vermicompost leachate formulations with BG11 medium and deionized water, systematically analyzing the effects of varying ratios on the growth kinetics of Chlorella pyrenoidosa. The results revealed that C3 (earthworm feces extract∶water = 5∶5, V/V) demonstrated optimal industrial applicability within the multidimensional evaluation framework. While attaining a 91.40% reduction in unit production cost relative to BG11 controls, this formulation sustained superior production performance, evidenced by a biomass concentration of (1.340 ± 0.071)×107 cells/mL, accompanied by a 48.77% enhancement in specific growth rate and 84.67% decrease in attenuation rate compared to BG11 controls. These data substantiated that the developed formulation constitutes a technically viable approach for low-cost Chlorella pyrenoidosa production.

Key words: earthworm feces extract, Chlorella pyrenoidosa, BG11, population growth

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