湖北农业科学 ›› 2018, Vol. 57 ›› Issue (2): 80-82.doi: 10.14088/j.cnki.issn0439-8114.2018.02.020

• 园艺·特产 • 上一篇    下一篇

基于液体培养基的白芨种苗离体快繁技术

熊丙全1, 廖相建1, 郑雪莲2, 邓科君2, 阳淑1, 张勇2   

  1. 1.成都农业科技职业学院园林园艺分院,成都 611130;
    2.电子科技大学生命科学与技术学院,成都 610054
  • 收稿日期:2017-08-15 出版日期:2018-01-25 发布日期:2018-01-10
  • 通讯作者: 张 勇(1977-),男,甘肃武威人,教授,博士,主要从事植物生物技术研究,(电子信箱)zhangyong916@uestc.edu.cn。
  • 作者简介:熊丙全(1976-),男,四川新都人,副教授/高级农艺师,硕士,主要从事园艺植物及药用植物研究,(电话)18980716408(电子信箱)45973177@qq.com
  • 基金资助:
    四川省教育厅自然科学研究项目(2017ZB0069); 中央高校基本科研业务费资助项目(ZYGX2014J081); 成都农业科技职业学院科研项目CNY15-09)

A Rapid in vitro Propagation Technique of Bletilla striata Seedling Based on Liquid Culture Medium

XIONG Bin-quan1, LIAO Xiang-jian1, ZHENG Xue-lian2, DENG Ke-jun2, YANG Shu1, ZHANG Yong2   

  1. 1.Garden and Horticulture Branch, Chengdu Agricultural Science and Technology Career Academy, Chengdu 611130, China;
    2.College of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
  • Received:2017-08-15 Online:2018-01-25 Published:2018-01-10

摘要: 针对白芨[Bletilla striata (Thunb.)Reichb.f.]种苗人工繁育存在的问题,介绍了一种基于液体培养基的白芨种苗离体快繁技术,比较了不同成熟度白芨种子离体培养的具体技术方案。该技术操作简便,成本低廉,能有效实现白芨组培苗的高效快速生产,适宜于进行规模化生产。

关键词: 白芨[Bletilla striata (Thunb.)Reichb.f.], 组织培养苗, 液体培养基, 离体快繁

Abstract: A rapid in vitro propagation technique based on liquid culture medium for artificial breeding of Bletilla striata (Thunb.)Reichb.f. seedling was introduced aiming at solving the current problems. The specific techniques for in vitro culture of B. striata seeds with different maturity were compared. As practice confirms, the technique was with simple operation and low cost, which can effectively realize the rapid and efficient production of B. striata tissue culture seedling, thus was suitable for large scale production.

Key words: Bletilla striata (Thunb.)Reichb.f., tissue culture seedling, liquid culture medium, in vitro rapid propagation

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