进境美国高粱种子中产黄色镰孢菌的检测与鉴定

doi:10.14088/j.cnki.issn0439-8114.2019.08.017

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (8): 74-78.doi: 10.14088/j.cnki.issn0439-8114.2019.08.017

进境美国高粱种子中产黄色镰孢菌的检测与鉴定

王艺^1,2, 周密密¹, 孙民琴³, 郭晓驹³, 李彬¹, 吴翠萍¹

1.江苏出入境检验检疫局动植物与食品检测中心,南京 210019;
2.南京农业大学,南京 210009;
3.南通海关,江苏南通 222600

收稿日期:2018-09-17 出版日期:2019-04-25 发布日期:2019-12-04
通讯作者: 李彬（1972-）,男,主要从事植物检疫工作,（电话）13815871522（电子信箱）libin_1103@163.com
作者简介:王艺（1996-）,女,江苏泰州人,在读硕士研究生,研究方向为植物检疫,（电话）18305176727（电子信箱）2017802155@njau.edu.cn
基金资助:
国家质量监督检验检疫总局科技计划项目（2017IK155）; 江苏出入境检验检疫局科技计划项目（2017KJ08）

Detection and identification of Fusarium thapsinum on imported Sorghum bicolor seeds from USA

WANG Yi^1,2, ZHOU Mi-mi¹, SUN Min-qin³, GUO Xiao-ju³, LI Bin¹, WU Cui-ping¹

1.Animal,Plant and Food Identification Center,Jiangsu Entry-Exit Inspection and Quarantine Bureau,Nanjing 210019,China;
2.Nanjing Agricultural University,Nanjing 210009,China;
3.Nantong Customs,Nantong 222600,Jiangsu,China

Received:2018-09-17 Online:2019-04-25 Published:2019-12-04

摘要/Abstract

摘要： 从进境的美国高粱种子中分离到一株疑似产黄色镰孢菌（Fusarium thapsinum）的菌株A009。该菌株菌落形态和显微特征均与产黄色镰孢菌一致。基于双基因位点（ITS、EF-1α）进行最大似然分析,构建系统发育树。结果表明,菌株A009与Fusarium thapsinum（Gibberella thapsina）聚集在一个分支上,最大相似度为100%。致病性测试表明,菌株A009接种高粱幼苗叶片,4 d后在接种部位出现萎蔫症状。根据上述试验结果,将进境美国高粱种子中的菌株A009鉴定为产黄色镰孢菌。

关键词: 产黄色镰孢菌（Fusarium thapsinum）, 高粱病害, 形态特征, 系统发育分析, 致病性测试

Abstract: The isolate A009 was obtained from Sorghum bicolor seeds imported from America. It was similar to Fusarium thapsinum in morphology. The results of phylogenetic analysis based on double gene（ITS and EF-1α） locuses showed the isolate A009 clustered within the type strain of Fusarium thapsinum （Gibberella thapsina）. The maximum likelihood was 100%. Pathogenicity test showed that it caused leaf spot and wilt on inoculated sorghum seedlings after 4 days. Based on all above results, the isolate A009 was identified as Fusarium thapsinum.

Key words: Fusarium thapsinum, sorghum diseases, morphological characters, phylogenetic analysis, pathogenicity test

中图分类号:

S41-30

王艺, 周密密, 孙民琴, 郭晓驹, 李彬, 吴翠萍. 进境美国高粱种子中产黄色镰孢菌的检测与鉴定[J]. 湖北农业科学, 2019, 58(8): 74-78.

WANG Yi, ZHOU Mi-mi, SUN Min-qin, GUO Xiao-ju, LI Bin, WU Cui-ping. Detection and identification of Fusarium thapsinum on imported Sorghum bicolor seeds from USA[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(8): 74-78.

参考文献

[1] DENDY D A V. Sorghum and millets:Chemistry and technology[J].Trends in food science & technology,1996,7(3):101.
[2] 张向民. 镰刀菌属分类学研究历史与现状[J].菌物研究,2005, 3(2):59-62.
[3] MARASAS W F O,RHEEDER J P,LAMPRECHT S C,et al. Fusarium andiyazi sp. nov.,a new species from sorghum[J].Mycologia,2001,93(6):1203-1210.
[4] CAB International.Crop protection compendium[M].Wallingford,UK:CAB International,2007.
[5] YLI-MATTILA T,MACH R L,ALEKHINA I A,et al.Phylogenetic relationship of Fusarium langsethiae to Fusarium poae and Fusarium sporotrichioides as inferred by IGS,ITS,β-tubulin sequences and UP-PCR hybridization analysis[J].International journal of food microbiology,2004,95(3):267-285.
[6] YLI-MATTILA T,PAAVANEN-HUHTALA S,BULAT S,et al.Molecular morphological and phylogenetic analysis of the Fusarium avenaceum/F. arthrosporioides/F. tricinctum species complex—A polyphasic approach[J].Mycological research,2002,106(6):655-669.
[7] SCHROERS H J,O’DONNELL K,LAMPRECHT S C,et al. Taxonomy and phylogeny of the Fusarium dimerum species group[J].Mycologia,2009,101(1):44-70.
[8] 曹雪梅,李生兵,张惠玲,等.甘草根腐病病原菌鉴定[J].植物病理学报,2014,44(2):213-216.
[9] WHITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics[A].INNIS M A,GELFAND D H,SNINSKY J J,et al. PCR protocols. A guide to methods and applications[C].San Diego:Academic,1990.315-322.
[10] O’DONNELL K,KISTLER H C,CIGELNIK E,et al. Multiple evolutionary origins of the fungus causing Panama disease of banana:Concordant evidence from nuclear and mitochondrial gene genealogies[J].Proceedings of the national academy of sciences,1998,95(5):2044-2049.
[11] THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The Clustal_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic acids research,1997,25(24):4876-4882.
[12] TAMURA K,NEI M.Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees[J].Molecular biology and evolution,1993,10(3):512-526.
[13] KLITTICH C J R,LESLIE J F,NELSON P E,et al. Fusarium thapsinum (Gibberella thapsina):A new species in section Liseola from sorghum[J].Mycologia,1997,89(4):643-652.
[14] 柳凤,詹儒林,韦继光,等.现代生物技术在镰刀菌分类学中的应用[J].中国农学通报,2012,28(30):166-170.
[15] KRISTENSEN R,TORP M,KOSIAK B,et al.Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 a gene sequences[J].Mycological Research,2005,109(2):173-186.
[16] 卢维宏,黄思良,陶爱丽,等.玉米穗腐病样品中层出镰刀菌的分离与鉴定[J].植物保护学报,2011,38(3):233-239.
[17] 易铭,梁嘉俊,史建,等.采用EF-1α序列分析法对苜蓿根腐病病原菌——锐顶镰刀菌的鉴定[J].草业学报,2017,26(2):61-68.
[18] 雷娅红,况卫刚,郑春生,等.基于DNA条形码技术对镰刀菌属的检测鉴定[J].植物保护学报,2016,43(4):544-551.
[19] SAMPIETRO D A,MARIN P,IGLESIASA J,et al.A molecular based strategy for rapid diagnosis of toxigenic Fusarium species associated to cereal grains from Argentina[J].Fungal biology,2010,114:74-81.
[20] 胡兰,姜钰,徐秀德.基于形态学和EF-1α序列特征的我国高粱子粒寄藏镰孢菌种群鉴定[J].作物杂志,2013(4):129-132.

进境美国高粱种子中产黄色镰孢菌的检测与鉴定

Detection and identification of Fusarium thapsinum on imported Sorghum bicolor seeds from USA

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	吕言, 周俊, 胡冲, 石鹏. 重庆彭水县中华蜜蜂形态指标测定及比较研究[J]. 湖北农业科学, 2021, 60(5): 100-102.
[2]	王佐乾, 潘竹青, 张舒, 游景茂, 王友平, 杨小林. 湖北省黄连炭疽病的病原鉴定及其生物学特性[J]. 湖北农业科学, 2020, 59(24): 117-121.
[3]	李莉云, 刘兴乐, 杨青, 余华强. 大马士革玫瑰花芽分化的研究[J]. 湖北农业科学, 2020, 59(21): 95-98.