湖北农业科学 ›› 2019, Vol. 58 ›› Issue (22): 104-107.doi: 10.14088/j.cnki.issn0439-8114.2019.22.023

• 植物保护 • 上一篇    下一篇

番茄叶斑病和果斑病病原鉴定及生物学特性研究

杨绍丽, 吴仁锋   

  1. 武汉市农业科学院蔬菜研究所,武汉 430065
  • 收稿日期:2019-08-10 出版日期:2019-11-25 发布日期:2019-12-09
  • 通讯作者: 吴仁锋,男,正高职高级农艺师,主要从事蔬菜病害诊断及综合防控方面的研究,(电子信箱)wuhanwurenfeng@126.com。
  • 作者简介:杨绍丽(1981-),女,湖北枣阳人,农艺师,硕士,主要从事蔬菜病害诊断及防控技术研究,(电话)18971570185(电子信箱) yangshaoli0123@163.com。
  • 基金资助:
    武汉市农业科学院科技创新项目(武农科政办[2019]6号)

Pathogen identification and biological characteristics of pathogens of tomato leaf spot and fruit spot disease

YANG Shao-li, WU Ren-feng   

  1. Institute of Vegetable,Wuhan Academy of Agricultural Sciences,Wuhan 430065,China
  • Received:2019-08-10 Online:2019-11-25 Published:2019-12-09

摘要: 从武汉市番茄大棚番茄叶片和果实上分离出引起番茄叶斑病和果斑病的病原真菌,对该菌进行了病原菌鉴定和生物学特性研究。结果表明,经形态学观察、致病性测定及分子鉴定该病原菌为多主棒孢霉(Corynespora cassiicola)。对该菌株生物学特性研究表明,该病原菌喜光照,最适宜生长的温度为25~30 ℃,生长适宜pH为7~9,喜欢弱碱性生长环境,供试5种碳源中葡萄糖的利用效果最好,5种氮源中硝酸钾利用效果最好。

关键词: 番茄叶斑病, 番茄果斑病, 多主棒孢霉(Corynespora cassiicola), 生物学特性

Abstract: Pathogenic fungi causing tomato leaf spot and fruit spot disease were isolated from tomato leaves and fruits in greenhouse of Wuhan city. The pathogen identification and biological characteristics were studied. The results showed that the pathogen of tomato leaf spot and fruit spot disease was identified as Corynespora cassiicola by morphological observation, pathogenicity determination and molecular identification. The biological characteristics of the strain showed that the pathogen liked light, the optimum temperature for growth was 25~30 ℃, the optimum pH was 7~9, and the weak alkaline growth environment was preferred. Among the five carbon sources, glucose had the best utilization effect, and potassium nitrate had the best utilization effect among the five nitrogen sources.

Key words: tomato leaf spot, tomato fruit spot disease, Corynespora cassiicola, biological characteristics

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