菠菜SpNHX1基因的克隆及生物信息学分析

doi:10.14088/j.cnki.issn0439-8114.2019.23.052

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 211-214.doi: 10.14088/j.cnki.issn0439-8114.2019.23.052

菠菜SpNHX1基因的克隆及生物信息学分析

张海洋, 付娆, 李茹霞, 顾寅钰, 梁晓艳, 宋延静, 李萌, 王向誉, 郭洪恩

山东省蚕业研究所,山东烟台 264002

收稿日期:2019-02-21 出版日期:2019-12-10 发布日期:2019-12-18
通讯作者: 郭洪恩（1969-）,男,研究员,主要从事设施农业及节水灌溉技术研究,（电子信箱） guohongen163@163.com。
作者简介:张海洋（1989-）,男,山东临朐人,助理研究员,博士,主要从事耐盐蔬菜选育及其优异性状形成的分子机理研究,（电话）18053646784（电子信箱）oceanz_zhy@163.com
基金资助:
烟台市科技计划项目（2018ZHGY081）; 山东省重点研发计划项目（GG201706190001）; 山东省农业科学院农业科技创新工程项目（CXGC2016B10; CXGC2018F6）

Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.

ZHANG Hai-yang, FU Rao, LI Ru-xia, GU Yin-yu, LIANG Xiao-yan, SONG Yan-jing, LI Meng, WANG Xiang-yu, GUO Hong-en

Shandong Institute of Sericulture,Yantai 264002,Shandong,China

Received:2019-02-21 Online:2019-12-10 Published:2019-12-18

摘要/Abstract

摘要： 从菠菜（Spinacia oleracea L.）栽培种中克隆得到1个NHX1基因,命名为SpNHX1。利用生物信息学软件对获得的基因核苷酸序列及编码的蛋白质序列进行分析,结果发现SpNHX1属于Vac亚家族,序列长度为6 090 bp,开放阅读框为1 662 bp,编码553个氨基酸残基。利用相关软件或在线工具对SpNHX1进行生物信息学分析,结果表明其编码蛋白分子式为C_{2 818}H_{4 363}N₆₉₇O₇₇₂S₂₅,属于稳定的疏水性蛋白;进化树分析表明SpNHX1蛋白与拟南芥AtNHX1、AtNHX2亲缘关系较近,属于定位在液泡膜上的蛋白,SpNHX1蛋白与双子叶植物的NHX蛋白亲缘关系较近。

关键词: 菠菜（Spinacia oleracea L.）, 耐盐, SpNHX1, 基因克隆, 生物信息学分析

Abstract: NHX1 gene was cloned from cultivated spinach （Spinacia oleracea L.） and named SpNHX1. Bioinformatics software were used to analyze the obtained gene nucleotide sequences and protein sequences encoded. The results showed that SpNHX1 belonged to the Vac subfamily, with a sequence length of 6 090 bp, an open reading frame of 1 662 bp, and 553 amino acid residues encoded. The bioinformatics analysis of SpNHX1 was carried out by using relevant software or online tools. The results showed that the molecular formula of its coding protein was C_{2 818}H_{4 363}N₆₉₇O₇₇₂S₂₅, which was a stable hydrophobic protein. Evolutionary tree analysis shows that SpNHX1 is closely related to AtNHX1 and AtNHX2 proteins of Arabidopsis thaliana, which belong to proteins located on vacuolar membranes. SpNHX1 protein is closely related to NHX proteins of dicotyledons.

Key words: Spinacia oleracea L., salt tolerance, SpNHX1, gene cloning, bioinformatic analysis

中图分类号:

S636.1

张海洋, 付娆, 李茹霞, 顾寅钰, 梁晓艳, 宋延静, 李萌, 王向誉, 郭洪恩. 菠菜SpNHX1基因的克隆及生物信息学分析[J]. 湖北农业科学, 2019, 58(23): 211-214.

ZHANG Hai-yang, FU Rao, LI Ru-xia, GU Yin-yu, LIANG Xiao-yan, SONG Yan-jing, LI Meng, WANG Xiang-yu, GUO Hong-en. Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(23): 211-214.

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菠菜SpNHX1基因的克隆及生物信息学分析

Cloning and bioinformatic analysis of SpNHX1 gene from Spinacia oleracea L.

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