Cep164基因对CP110在中心粒上表达的影响

doi:10.14088/j.cnki.issn0439-8114.2020.02.037

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (2): 166-168.doi: 10.14088/j.cnki.issn0439-8114.2020.02.037

Cep164基因对CP110在中心粒上表达的影响

王春花

牡丹江师范学院生命科学与技术学院,黑龙江牡丹江 157000

收稿日期:2019-01-12 发布日期:2020-04-24
作者简介:王春花（1984-）,女,黑龙江牡丹江人,讲师,博士,主要从事药理与细胞生物学研究,（电话）13614631953（电子信箱）swxwch@126.com
基金资助:
黑龙江省普通本科高等学校青年创新人才培养计划; 牡丹江师范学院青年创新人才培养项目（QC201601）; 牡丹江师范学院博士启动资金项目（MNUB201407）

The Cep164 gene indirectly regulate CP110 expression in the centriole

WANG Chun-hua

School of Life Science and Technology,Mudajiang Normal University,Mudanjiang 157000,Heilongjiang,China

Received:2019-01-12 Published:2020-04-24

摘要/Abstract

摘要： Cep164基因是参与纤毛形成的重要基因之一,为研究Cep164基因与CP110的关系,探讨CP110在Cep164调节纤毛形成中的作用,通过免疫荧光染色与Western印迹法确定Cep164基因缺失时CP110基因的表达情况,利用免疫共沉淀法确认Cep164与CP110蛋白相互作用的关系。结果表明,Cep164基因的缺失导致CP110表达增高,Cep164与CP110蛋白无直接相互作用关系,Cep164是通过间接方式调控CP110在细胞内表达,CP110降解失败可能为Cep164基因缺失导致纤毛生成障碍的原因之一。

关键词: Cep164基因, CP110基因, 中心粒

Abstract: Cep164 is one of the important genes involved in ciliogenesis. In order to study the relationship of the Cep164 and CP110, explored the role of CP110 in the regulation of ciliogenesis by Cep164. Immunofluorescence staining and Western Blotting were used to determine the expression of CP110 gene in the Cep164 gene knockout cell and embryo of mice. The interaction between the Cep164 and CP110 protein was confirmed by COIP. The results showed the Cep164 gene mutation increased CP110 gene expression. Cep164 regulated the expression of CP110 in cells indirectly. CP110 degradation failure could be the one of reason for the Cep164 gene mutation cause cilia dysfunction.

Key words: Cep164 gene, CP110 gene, centriole

中图分类号:

Q132.7

王春花. Cep164基因对CP110在中心粒上表达的影响[J]. 湖北农业科学, 2020, 59(2): 166-168.

WANG Chun-hua. The Cep164 gene indirectly regulate CP110 expression in the centriole[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(2): 166-168.

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Cep164基因对CP110在中心粒上表达的影响

The Cep164 gene indirectly regulate CP110 expression in the centriole

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