重庆市豌豆枯萎病病原菌的分离与鉴定

doi:10.14088/j.cnki.issn0439-8114.2020.06.016

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (6): 84-87.doi: 10.14088/j.cnki.issn0439-8114.2020.06.016

重庆市豌豆枯萎病病原菌的分离与鉴定

张慧, 马连杰, 冯牧野, 卢文才, 廖敦秀

重庆市农业科学院农业资源与环境研究所,重庆 401329

收稿日期:2019-06-19 出版日期:2020-03-25 发布日期:2020-06-13
通讯作者: 廖敦秀（1966-）,女,研究员,主要从事农业环境资源研究工作,（电子信箱）664852751@qq.com。
作者简介:张慧（1989-）,女,江苏睢宁人,助理研究员,硕士,主要从事植物保护研究工作,（电话）18581465257（电子信箱）1501380987@qq.com;
基金资助:
重庆市良种创新暨重大科技推广项目（NKY-2016AB009）

Isolation and identification of pathogen of pea fusarium wilt in Chongqing city

ZHANG Hui, MA Lian-jie, FENG Mu-ye, LU Wen-cai, LIAO Dun-xiu

Institute of Agricultural Resources and Environment,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China

Received:2019-06-19 Online:2020-03-25 Published:2020-06-13

摘要/Abstract

摘要： 采用组织分离法,对重庆市豌豆枯萎病病原菌进行分离和纯化,结合形态学和基因序列分析对分离物进行鉴定,采用无伤接种法测定分离物对豌豆离体叶片、茎秆的致病性。结果表明,从枯萎病样品中分离获得5株菌株,菌落为圆形,初期为白色絮状,后期呈黄棕色至褐色;供试菌株的ITS序列和G3PDH基因序列与Ascochyta pinodes的同源性达到99%;致病性测定结果表明,供试菌株对豌豆叶片及茎秆均有致病性。表明引起重庆市豌豆枯萎病的病原菌为Ascochyta pinodes。

关键词: 豌豆, 豌豆枯萎病, 病原鉴定, Ascochyta pinodes

Abstract: The tissue isolation method was used to isolate and purify the pathogen of spot of fusarium wilt of peas in Chongqing city, and the isolates were identified by combining with the analysis of morphology and gene sequence. The pathogenicity of the isolates to the leaves and stems of isolated peas was determined by the method of inoculation without injury. The results showed that five strains were isolated from infected leaves. The colony was round, white flocculent in the early stage and yellowish brown to brown in the later stage; The homology of ITS sequence and G3PDH gene sequences of the tested strain reached 99% with that of Ascochyta pinodes; The results of pathogenicity determination showed that the strain had pathogenicity to both leaves and stems of pea. It indicated that Ascochyta pinodes was the cause of pea blight in Chongqing city.

Key words: pea, pea fusarium wilt, pathogens identified, Ascochyta pinodes

中图分类号:

S436.43

张慧, 马连杰, 冯牧野, 卢文才, 廖敦秀. 重庆市豌豆枯萎病病原菌的分离与鉴定[J]. 湖北农业科学, 2020, 59(6): 84-87.

ZHANG Hui, MA Lian-jie, FENG Mu-ye, LU Wen-cai, LIAO Dun-xiu. Isolation and identification of pathogen of pea fusarium wilt in Chongqing city[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(6): 84-87.

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重庆市豌豆枯萎病病原菌的分离与鉴定

Isolation and identification of pathogen of pea fusarium wilt in Chongqing city

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