褪黑素抑制玉米赤霉烯酮诱导的猪卵巢颗粒细胞凋亡

doi:10.14088/j.cnki.issn0439-8114.2020.22.031

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (22): 151-154.doi: 10.14088/j.cnki.issn0439-8114.2020.22.031

褪黑素抑制玉米赤霉烯酮诱导的猪卵巢颗粒细胞凋亡

周佳伟¹, 吴俊静¹, 乔木¹, 王孝忠², 刘贵生¹, 梅书棋¹, 彭先文¹

1.湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉 430064;
2.湖北省宜昌市动物疫病预防控制中心,湖北宜昌 443000

收稿日期:2020-07-02 出版日期:2020-11-25 发布日期:2020-12-30
通讯作者: 彭先文(1974-),男,研究员,主要从事猪遗传育种研究,(电子信箱)pxwpal@163.com
作者简介:周佳伟(1989-),男,江西瑞昌人,助理研究员,博士,主要从事猪遗传育种研究,(电话)18674037082(电子信箱)422374839@qq.com
基金资助:
湖北省技术创新专项重大项目(2019ABA084、2017ABA134);湖北省自然科学基金创新群体项目(2018CFA014);国家生猪产业技术体系项目(CARS-35)

Melatonin inhibits zearalenone-induced apoptosis of porcine ovarian granulosa cells

ZHOU Jia-wei¹, WU Jun-jing¹, QIAO Mu¹, WANG Xiao-zhong², LIU Gui-sheng¹, MEI Shu-qi¹, PENG Xian-wen¹

1. Hubei Key Laboratory of Animal Embryo and Molecular Breeding/Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430063,China;
2. Animal Disease Prevention and Control Center of Yichang City,Yichang 443000,Hubei,China

Received:2020-07-02 Online:2020-11-25 Published:2020-12-30

摘要/Abstract

摘要： 以原代培养的猪卵巢颗粒细胞为模型,探究玉米赤霉烯酮(ZEA)对猪卵巢颗粒细胞凋亡的影响,并检测褪黑素(MT)在调节ZEA对猪卵巢颗粒细胞凋亡中的作用。结果表明,3和10 μmol/L的ZEA对猪卵巢颗粒凋亡的影响不显著,但30、50和100 μmol/L的ZEA显著促进猪卵巢颗粒细胞的凋亡,且呈现剂量效应关系;0.1和1 μmol/L的MT抑制30 μmol/L的ZEA诱导猪卵巢颗粒细胞的凋亡,能够提高细胞活力。

关键词: 玉米赤霉烯酮, 褪黑素, 猪卵巢颗粒细胞, 细胞凋亡

Abstract: Primary cultured porcine ovarian granulosa cells were used as a model to explore the effect of ZEA on the apoptosis of porcine ovarian granulosa cells,and to determine whether melatonin (MT) regulated the effect of ZEA on the apoptosis of porcine ovarian granulosa cells.The results showed that3 and10 μmol/L ZEA had no significant effect on the apoptosis of porcine ovarian granulosa cells,but30,50 and100 μmol/L ZEA significantly promoted the apoptosis of porcine ovarian granulosa cells,and showed a dose-effect relationship;It was found that 0.1 and1 μmol/L MT partially inhibited30 μmol/L ZEA-induced apoptosis of porcine ovarian granulosa cells and increased cell viability.

Key words: zearalenone, melatonin, porcine ovarian granulosa cells, cell apoptosis

中图分类号:

S858.28

周佳伟, 吴俊静, 乔木, 王孝忠, 刘贵生, 梅书棋, 彭先文. 褪黑素抑制玉米赤霉烯酮诱导的猪卵巢颗粒细胞凋亡[J]. 湖北农业科学, 2020, 59(22): 151-154.

ZHOU Jia-wei, WU Jun-jing, QIAO Mu, WANG Xiao-zhong, LIU Gui-sheng, MEI Shu-qi, PENG Xian-wen. Melatonin inhibits zearalenone-induced apoptosis of porcine ovarian granulosa cells[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 151-154.

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褪黑素抑制玉米赤霉烯酮诱导的猪卵巢颗粒细胞凋亡

Melatonin inhibits zearalenone-induced apoptosis of porcine ovarian granulosa cells

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