朱砂叶螨各发育阶段内参基因评估及筛选

doi:10.14088/j.cnki.issn0439-8114.2021.11.030

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (11): 141-145.doi: 10.14088/j.cnki.issn0439-8114.2021.11.030

朱砂叶螨各发育阶段内参基因评估及筛选

倪婧, 谢道燕, 江秀均, 杨振国, 柴建萍

云南省农业科学院蚕桑蜜蜂研究所,云南蒙自 661101

收稿日期:2020-09-22 出版日期:2021-06-10 发布日期:2021-07-15
作者简介:倪婧（1987-）,女（彝族）,云南建水人,助理研究员,硕士,主要从事植物保护研究,（电话）13988003606（电子信箱）nixiaoxian@126.com。
基金资助:
云南省农业联合专项（2017FG001-094）

Evaluation and screening of internal reference genes of Tetranychus cinnabarinus at different developmental stages

NI Jing, XIE Dao-yan, JIANG Xiu-jun, YANG Zhen-guo, CHAI Jian-ping

Institute of Sericulture ＆ Apiculture,Yunnan Academy of Agricultural Sciences,Mengzi 661101,Yunnan,China

Received:2020-09-22 Online:2021-06-10 Published:2021-07-15

摘要/Abstract

摘要： 在实时荧光定量PCR试验中需引入一个或几个表达稳定的内参基因作为参照物对表达结果进行校正和标准化处理,但并非所有内参基因都完美地适用于任何特定试验条件下的生物样本,要得到准确、真实的定量结果,就需要筛选与试验匹配的最佳内参基因。研究通过实时荧光定量技术,利用geNorm软件分析,对朱砂叶螨敏感品系及抗炔螨特品系各个发育阶段中6个常用内参基因的表达稳定性进行评估。结果表明,两个品系间RPS18与α-TUB内参基因组合表达稳定度较高;不同发育阶段α-TUB与GAPDH内参基因组合表达稳定度较高。

关键词: 朱砂叶螨, 实时荧光定量PCR, 内参基因, 评估及筛选

Abstract: In the real-time fluorescent quantitative PCR experiments need to introduce one or a few stable reference genes as a reference for expression result correction and standardization, but not all reference genes are perfect for any specific biological samples under special experiment conditions, in order to get accurate results, We need to screen the best reference genes match with the experiment. In this study, real-time fluorescence quantitative PCR and geNorm software were used to analysis and evaluate the stability of six commonly used reference genes in the propargite resistance and sensitive Tetranychus cinnabarinus. The result showed that RPS18 and α-TUB reference genes showed high expression stability in this two strains,and α-TUB and GAPDH reference genes showed high expression stability in different developmental stages.

Key words: Tetranychus cinnabarinus, real-time fluorescence quantitative PCR, reference genes, evaluation and screening

中图分类号:

Q789
S433.7

倪婧, 谢道燕, 江秀均, 杨振国, 柴建萍. 朱砂叶螨各发育阶段内参基因评估及筛选[J]. 湖北农业科学, 2021, 60(11): 141-145.

NI Jing, XIE Dao-yan, JIANG Xiu-jun, YANG Zhen-guo, CHAI Jian-ping. Evaluation and screening of internal reference genes of Tetranychus cinnabarinus at different developmental stages[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(11): 141-145.

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朱砂叶螨各发育阶段内参基因评估及筛选

Evaluation and screening of internal reference genes of Tetranychus cinnabarinus at different developmental stages

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