湖北农业科学 ›› 2021, Vol. 60 ›› Issue (24): 46-50.doi: 10.14088/j.cnki.issn0439-8114.2021.24.011

• 资源·环境 • 上一篇    下一篇

虾稻共作田有机质降解菌的筛选和评价

郑娇莉, 姚经武, 黄大野, 杨丹, 王蓓蓓, 曹春霞   

  1. 湖北省生物农药工程研究中心,武汉 430064
  • 收稿日期:2021-10-19 出版日期:2021-12-25 发布日期:2022-01-04
  • 通讯作者: 曹春霞,研究员,主要从事生物防治研究,(电子信箱)Cao-chunxia@163.com。
  • 作者简介:郑娇莉(1989-),女,四川遂宁人,助理研究员,博士,主要从事环境微生物与环境修复研究,(电话)13297082609(电子信箱)zhengjl150@126.com;
  • 基金资助:
    湖北省水稻“三优”科技创新行动项目

Screening and evaluation of organic matter degrading bacteria in rice-crayfish co-culture (RCIS)

ZHENG Jiao-li, YAO Jing-wu, HUANG Da-ye, YANG Dan, WANG Bei-bei, CAO Chun-xia   

  1. Hubei Biopesticide Engineering Research Center,Wuhan 430064,China
  • Received:2021-10-19 Online:2021-12-25 Published:2022-01-04

摘要: 从虾稻共作养殖环境中筛选6株有机质降解菌,经鉴定均为芽孢杆菌(Bacillus sp.),其中,OC-1、OC-2、OC-3和OC-6 4株细菌均归属于阿氏芽孢杆菌(Bacillus aryabhatta),OC-4鉴定为弯曲芽孢杆菌(Bacillus flexus),OC-5鉴定为黄海芽孢杆菌(Bacillus marisflavi),这些菌株均能高效降解饵料浸出液培养基中的有机质和氨氮,培养3 d后COD降解率均为85%以上,其中,OD-1、OD-2对有机质的降解速率和降解效率最高,这可能与其具备多种类胞外酶分泌能力有关,这2种菌株具有一定的应用潜力。

关键词: 虾稻共作, 有机质降解, 胞外酶

Abstract: Six strains of organic matter degrading bacteria were screened from environment of rice-crayfish co-culture(RCIS), all of which were identified as Bacillus sp.. Among them, OC-1, OC-2, OC-3 and OC-6 were all classified as Bacillus aryabhatta, OC-4 was identified as Bacillus flexus, and OC-5 was identified as Bacillus marisflavi. These strains can efficiently degrade organic matter andammonia in the bait culture medium, and the COD degradation rate of these strains was more than 85% after three days of cultivation. OD-1, OD-2 can quickly and efficiently degrade the organic matter in bait culture medium. The high degradation efficiency may be related to its ability to secrete a variety of extracellular enzymes. These two strains had application potential.

Key words: rice-crayfish co-culture (RCIS), organic matter degrading bacteria, extracellular enzymes

中图分类号: