湖北农业科学 ›› 2022, Vol. 61 ›› Issue (13): 161-164.doi: 10.14088/j.cnki.issn0439-8114.2022.13.030

• 生物工程 • 上一篇    下一篇

铁皮石斛组织培养研究

蔡正旺a,b   

  1. 安康学院,a.电子与信息工程学院; b.现代农业与生物科技学院,陕西 安康 725000
  • 收稿日期:2021-09-08 出版日期:2022-07-10 发布日期:2022-08-10
  • 作者简介:蔡正旺(1977-),男,陕西安康人,助理研究员,硕士,主要从事生物资源的研究,(电话)13772223205(电子信箱)707083838@qq.com
  • 基金资助:
    陕西省科技厅项目资助(2015NY135)

Study on tissue culture of Dendrobium officinale

CAI Zheng-wanga,b   

  1. a. College of Electronic & Information Engineering; b. College of Modern Agriculture and Biotechnology, Ankang University, Ankang 725000, Shaanxi, China
  • Received:2021-09-08 Online:2022-07-10 Published:2022-08-10

摘要: 以铁皮石斛(Dendrobium officinale Kimura et Migo)幼嫩带芽茎段作为试验材料,利用植物组织培养技术研究生长调节剂配比、天然添加物香蕉泥对铁皮石斛腋芽的诱导、增殖的影响;将诱导出的组培苗移栽到不同栽培基质中进行移栽驯化。结果表明,最适茎段腋芽诱导的培养基为MS+1.0 mg/L 6-BA+0.5 mg/L NAA+香蕉泥100 g/L;适合芽增殖的培养基为MS+2.0 mg/L 6-BA+0.5 mg/L NAA+香蕉泥100 g/L;铁皮石斛组培苗最适移栽单一基质为锯末,复合基质为松树皮与锯末,体积比为7∶3。

关键词: 铁皮石斛(Dendrobium officinale Kimura et Migo), 植物组织培养, 基本培养基, 驯化栽培

Abstract: The young shoots with buds of Dendrobium officinale were used as experimental materials to study the effects of hormone ratio and natural additive banana mud on the induction and proliferation of axillary buds of Dendrobium officinale by plant tissue culture technology. The induced tissue culture seedlings were transplanted into different cultivation substrates for transplanting and domestication. The results showed that the optimum medium for axillary bud induction was MS+1.0 mg/L 6-BA+0.5 mg/L NAA+banana mud 100 g/L; the suitable medium for bud proliferation was MS+2.0 mg/L 6-BA+0.5 mg/L NAA+banana mud 100 g/L; the optimal transplanting substrate for Dendrobium officinale plantlets was sawdust, and the composite substrate was pine bark and sawdust with a volume ratio of 7∶3.

Key words: Dendrobium officinale Kimura et Migo, plant tissue culture, basic medium, domestication cultivation

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