葛根总黄酮提取工艺优化及其抑菌与抗氧化活性研究

doi:10.14088/j.cnki.issn0439-8114.2023.11.012

湖北农业科学 ›› 2023, Vol. 62 ›› Issue (11): 65-72.doi: 10.14088/j.cnki.issn0439-8114.2023.11.012

葛根总黄酮提取工艺优化及其抑菌与抗氧化活性研究

郑敏^1,2, 苏福联^1,2, 袁名远^1,2, 刘玉^1,2, 黄东海¹, 张巍³, 李宇¹, 廖璐婧¹, 罗凯², 何美军¹

1.湖北省农业科学院中药材研究所/农业农村部中药材生物学与栽培重点实验室/湖北省中药材研发中心,湖北恩施 445000;
2.湖北民族大学生物与食品工程学院,湖北恩施 445000;
3.湖北省农业科学院畜牧兽医研究所,武汉 430064

收稿日期:2022-09-27 出版日期:2023-11-25 发布日期:2023-12-25
通讯作者: 何美军（1979-）,男,副研究员,主要从事药用植物天然产物开发及应用,（电子信箱）840940513@qq.com。
作者简介:郑敏（1996-）,女,重庆巴南人,在读硕士研究生,研究方向为农产品加工及贮藏,（电话）13452022924（电子信箱）2290539316@qq.com;罗凯（1979-）,男,副教授,主要从事食品化学及营养学研究,（电子信箱）80155782@qq.com。
基金资助:
湖北省农业科技创新中心2020年度重大科技研发项目（2020-620-000-002-04）; 湖北省现代农业产业技术体系（HBHZD-ZB-2020-005）; 恩施州科技计划项目（D20210006; D202100018）

Optimization of extraction process of total flavonoids from Pueraria montana and it’s antibacterial and antioxidant activities

ZHENG Min^1,2, SU Fu-lian^1,2, YUAN Ming-yuan^1,2, LIU Yu^1,2, HUANG Dong-hai¹, ZHANG Wei³, LI Yu¹, LIAO Lu-jing¹, LUO Kai², HE Mei-jun¹

1. Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences/Key Laboratory of Chinese Herbal Medicine Biology and Cultivation, Ministry of Agriculture and Rural Affairs/Hubei Research and Development Center of Chinese Herbal Medicine, Enshi 445000,Hubei,China;
2. College of Biological and Food Engineering, Hubei Minzu University, Enshi 445000,Hubei,China;
3. Institute of Animal Husbandry and Veterinary Research, Hubei Academy of Agricultural Sciences,Wuhan 430064, China

Received:2022-09-27 Online:2023-11-25 Published:2023-12-25

摘要/Abstract

摘要： 对葛根[Pueraria montana （Loureiro） Merrill]总黄酮的抑菌和抗氧化活性进行研究。采用单因素试验结合响应面法优化葛根的水提工艺,利用紫外分光光度法和高效液相色谱法对葛根总黄酮进行表征,并运用琼脂扩散法和以DPPH·、ABTS⁺·和总还原能力为指标评价葛根总黄酮的生物活性。结果表明,优化后的葛根总黄酮提取工艺为料液比（m∶V,g∶mL）1∶16,提取时间69 min,提取3次,总黄酮得率为7.690%。优化后的葛根总黄酮中检出9种常见黄酮成分,其含量总占比为91.81%;葛根总黄酮对11株供试菌有不同的抑菌活性,并首次发现其对粪链球菌（Enterococcus faecalis）和苏云金芽孢杆菌（Bacillus thuringiensis）有中度敏感作用,MIC分别为250、500 mg/mL,具有较强的清除能力和总还原能力,其中对DPPH·、ABTS⁺·自由基的IC₅₀分别为0.398、0.189 mg/mL。

关键词: 葛根[Pueraria montana （Loureiro） Merrill], 响应面, 工艺优化, 总黄酮, 抑菌, 抗氧化

Abstract: The antibacterial and antioxidant activities of total flavonoids from Pueraria montana （Loureiro） Merrill were studied. The water extraction technology of Pueraria montana was optimized by the single factor test and response surface method. The total flavonoids were characterized by ultraviolet spectrophotometry and high performance liquid chromatography. The biological activities of total flavonoids were evaluated by agar diffusion method, with DPPH·, ABTS⁺· and total reduction capacity as indicators. The results showed that the optimized extraction process of total flavonoids from Pueraria montana was as follows： Solid-liquid ratio （m∶V,g∶mL） of 1∶16, extraction time of 69 min, extraction times of 3 times, and the total flavonoids yield was 7.690%. Nine common flavonoids were detected in the optimized total flavonoids of Pueraria montana, the total content of which accounted for 91.81%. The total flavonoids of Pueraria montana had different antibacterial activities against 11 strains tested, and it was found to be moderately sensitive to Enterococcus faecalis and Bacillus thuringiensis for the first time, with MIC of 250, 500 mg/mL, respectively. It had strong scavenging ability and total reduction ability, and the IC₅₀ of DPPH· and ABTS⁺· free radicals were 0.398, 0.189 mg/mL, respectively.

Key words: Pueraria montana（Loureiro）Merrill, response surface, process optimization, total flavonoid, antibacterial, antioxidation

中图分类号:

R284.2

郑敏, 苏福联, 袁名远, 刘玉, 黄东海, 张巍, 李宇, 廖璐婧, 罗凯, 何美军. 葛根总黄酮提取工艺优化及其抑菌与抗氧化活性研究[J]. 湖北农业科学, 2023, 62(11): 65-72.

ZHENG Min, SU Fu-lian, YUAN Ming-yuan, LIU Yu, HUANG Dong-hai, ZHANG Wei, LI Yu, LIAO Lu-jing, LUO Kai, HE Mei-jun. Optimization of extraction process of total flavonoids from Pueraria montana and it’s antibacterial and antioxidant activities[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(11): 65-72.

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葛根总黄酮提取工艺优化及其抑菌与抗氧化活性研究

Optimization of extraction process of total flavonoids from Pueraria montana and it’s antibacterial and antioxidant activities

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