民族药水河剑水提和醇提取物急性毒性试验及抗炎镇痛有效部位筛选

doi:10.14088/j.cnki.issn0439-8114.2024.09.020

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (9): 119-124.doi: 10.14088/j.cnki.issn0439-8114.2024.09.020

民族药水河剑水提和醇提取物急性毒性试验及抗炎镇痛有效部位筛选

周楚惠, 邓青梅, 陈勇, 杨温玲, 韦培, 覃雪美

广西中医药大学广西中医药科学实验中心,南宁 530200

收稿日期:2023-09-18 出版日期:2024-09-25 发布日期:2024-09-30
通讯作者: 陈勇（1961-）,男,江西南昌人,教授,主要从事中药民族药质量分析研究,（电子信箱）cy6381@163.com。
作者简介:周楚惠（1998-）,女,山东济宁人,在读硕士研究生,研究方向为中药质量分析,（电话）17860632091（电子信箱）545061701@qq.com。
基金资助:
广西一流学科建设立项课题（2018xk060; 2019xk105）; 广西壮瑶药重点实验室开放课题（GXZYKF2022-11）; 广西中药药效研究重点实验室研究课题（19-050-39-A6）; 广西高校中药提取纯化与质量分析重点实验室项目（桂教科研[2014]6号）

Acute toxicity test of water extract and alcohol extract of ethnic medicine Diplazium lanceum and screening of anti-inflammatory and analgesic effective parts

ZHOU Chu-hui, DENG Qing-mei, CHEN Yong, YANG Wen-ling, WEI Pei, QIN Xue-mei

Guangxi Scientific Research Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine,Nanning 530200, China

Received:2023-09-18 Published:2024-09-25 Online:2024-09-30

摘要/Abstract

摘要： 对水河剑（Diplazium lanceum）药材水提物及醇提物的急性毒性进行初步研究,并筛选水河剑药材具有抗炎镇痛效果的极性部位。使用最大给药量测定方法对小鼠灌胃给药,得到水河剑水提物及醇提物的最大耐受量（MTD）,考察水河剑用药的安全性;采用小鼠醋酸扭体及热板法考察水河剑不同极性部位的镇痛效果;采用小鼠耳肿胀法考察水河剑药材不同极性部位的抗炎效果;用ELISA法,测乙酸乙酯提取物（高、中、低）对小鼠耳部二甲苯致炎血清中炎症因子含量的影响。结果表明,水河剑水提物及醇提物急性毒性试验中均未能得到半数致死量（LD₅₀）,水河剑药材水提物和醇提取物最大耐受量分别为238.0、165.2 g/kg;水河剑药材不同极性部位提取物对小鼠的舔足时间都有所延长;水河剑药材不同极性部位均对小鼠扭体有抑制效果,各组镇痛率为11.14%~40.36%,其中镇痛效果最好的为乙酸乙酯部位,其次为石油醚部位;水河剑药材不同部位均对小鼠耳肿胀有抑制效果,各组耳肿胀抑制率为24.71%~48.47%,乙酸乙酯及石油醚部位抗炎效果最好;乙酸乙酯高、中、低剂量组小鼠血清中肿瘤坏死因子（TNF-α）、白介素-1β（IL-1β）、前列腺素E₂（PGE₂）炎症因子含量与模型对照组相比均有所下降,乙酸乙酯低、中剂量组的IL-1β、PGE₂含量及乙酸乙酯低剂量组的TNF-α含量与模型对照组相比具有显著差异。

关键词: 水河剑（Diplazium lanceum）, 最大耐受量, 急性毒性试验, 抗炎镇痛, 有效部位

Abstract: The acute toxicity of water and alcohol extracts of Diplazium lanceum was preliminarily studied and the polar sites with anti-inflammatory and analgesic effects were screened. The maximum tolerable dose （MTD） of the water and alcohol extracts of Diplazium lanceum was obtained by gavage administration to mice using the maximum dosage measurement method, and the safety of Diplazium lanceum was investigated; the analgesic effect of different polar parts of Diplazium lanceum was investigated using mouse acetic acid twisting and hot plate method; the anti-inflammatory effects of different polar parts were investigated using the mouse ear swelling method; the effect of ethyl acetate extract （high, medium, low） on the levels of inflammatory factors in the serum of mice with xylene- induced ear inflammation was measured using ELISA method. The results showed that LD₅₀ was not obtained in the acute toxicity experiment of water extract and alcohol extract of Diplazium lanceum. The MTD of water extract and alcohol extract were 238.0 g/kg and 165.2 g/kg, respectively; the extracts of different polar parts of Diplazium lanceum prolonged the mice's licking time of feet; the different polar parts of Diplazium lanceum had inhibitory effects on mouse torsion, with analgesic rates ranging from 11.14% to 40.36% in each group. Among them, the ethyl acetate part had the best analgesic effect, followed by the petroleum ether part; different parts of Diplazium lanceum had inhibitory effects on mouse ear swelling, with inhibition rates ranging from 24.71% to 48.47% in each group, and ethyl acetate and petroleum ether parts had the best anti-inflammatory effects; the levels of tumor necrosis factor （TNF-α）, interleukin-1β （IL-1β）, and prostaglandin E₂（PGE₂） inflammatory factors in the serum of mice in the high, medium, and low dose groups of ethyl acetate decreased compared to the model control group. The levels of IL-1β and PGE₂ in the low and medium dose groups of ethyl acetate, as well as the level of TNF-α in the low dose group of ethyl acetate, showed significant differences compared to the model control group.

Key words: Diplazium lanceum, maximum tolerance, acute toxicity test, anti-inflammatory and analgesic, effective parts

中图分类号:

周楚惠, 邓青梅, 陈勇, 杨温玲, 韦培, 覃雪美. 民族药水河剑水提和醇提取物急性毒性试验及抗炎镇痛有效部位筛选[J]. 湖北农业科学, 2024, 63(9): 119-124.

ZHOU Chu-hui, DENG Qing-mei, CHEN Yong, YANG Wen-ling, WEI Pei, QIN Xue-mei. Acute toxicity test of water extract and alcohol extract of ethnic medicine Diplazium lanceum and screening of anti-inflammatory and analgesic effective parts[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(9): 119-124.

参考文献

[1] 中国科学院中国植物志编委会.中国植物志3卷2分册[M].北京:科学出版社,1999.
[2] 杨明惠,杨雪琼,汪国松,等.蹄盖蕨属植物化学成分和药理活性研究进展[J].中国药房,2008(15):1189-1191.
[3] 杨春燕,龙春林,石亚娜,等.广西靖西县端午药市的民族植物学研究[J].中央民族大学学报(自然科学版),2009,18(2):16-26.
[4] 郭小梅. 浅谈西药常见的不良反应及预防对策[J].当代医药论丛,2015,13(23):210-211.
[5] 孙倩倩,李红锐,陈毅坚,等.深绿短肠蕨抗氧化、降糖、抗炎活性研究[J].云南民族大学学报(自然科学版),2019,28(5):428-432.
[6] 陈靖,杨丽.槐胺碱镇痛抗炎作用及其机制研究[J].中华中医药学刊,2017,35(6):1424-1427.
[7] 蔡家驹,曾聪彦,梅全喜.火炭母水提物抗炎、镇痛作用的实验研究[J].时珍国医国药,2017,28(1):100-102.
[8] 张立军,戴海蓉,杨志军,等.高乌头炮制前后毒性部位与镇痛抗炎药效部位筛选研究[J].中药药理与临床,2017,33(4):78-82.
[9] 毕晓黎,陈雪,李素梅,等.葫芦钻水提物的抗炎镇痛作用及其急性毒性[J].中成药,2017,39(5):1057-1059.
[10] 魏春华,程虹毓,高燕萍,等.藏药矮紫堇解热镇痛抗炎作用的研究[J].中国新药杂志,2017,26(3):337-342.
[11] 魏春华,高燕萍,罗光明,等.藏药无尾果抗炎镇痛解热作用研究[J].中药材,2016,39(12):2875-2878.
[12] 韦琳,宗伟,曾庆鸿,等.花椒抗炎镇痛网络药理学分析及实验验证研究[J].中国中药杂志,2021,46(12): 3034-3042.
[13] 荆雅峰,汤红翠,李婧,等.金叶女贞花提取物镇痛抗炎活性的筛选及评价[J].神经解剖学杂志,2021,37(1):84-88.
[14] 张亚涛,包晓玮,曾兰君,等.刺山柑果实醇提物正丁醇部位提取物抗炎镇痛效果分析[J].新疆农业科学,2021,58(1):159-167.
[15] 廖娜,黄光伟,唐红艳,等.大血藤醇提物抗炎镇痛和止血活性研究初探[J].广西植物,2021,41(7):1120-1125.
[16] 李蒙,沈佳瑜,李听弦,等.苍耳子炮制前后的抗炎、镇痛作用比较[J].中国医院药学杂志,2017,37(3):232-234.
[17] 黄显章,丁生晨,袁林,等.蜣螂水提取物的镇痛抗炎作用[J].中华中医药学刊,2016,34(9):2191-2194.
[18] 陈根元,贾琦珍,邓利,等.小花棘豆提取物抗炎镇痛作用的研究[J].中国兽医杂志,2016,52(7):108-110.
[19] 梁生林,黄芳辉,钟兴华,等.乌蔹莓抗炎镇痛有效部位的筛选[J].中草药,2016,47(4):634-639.
[20] 王丽君,邓同兴,张景亮,等.白藜芦醇抗炎镇痛作用的COX₂/PGE₂信号通路机制研究[J].中药药理与临床,2017,33(4):35-38.

民族药水河剑水提和醇提取物急性毒性试验及抗炎镇痛有效部位筛选

Acute toxicity test of water extract and alcohol extract of ethnic medicine Diplazium lanceum and screening of anti-inflammatory and analgesic effective parts

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价