花椒精油成分分析及其对四种植物病原真菌的抗菌作用

doi:10.14088/j.cnki.issn0439-8114.2024.12.011

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (12): 62-66.doi: 10.14088/j.cnki.issn0439-8114.2024.12.011

花椒精油成分分析及其对四种植物病原真菌的抗菌作用

李承钊^1,2, 刘玉杰^1,2, 王伟峰^1,3, 孟宪华^1,3, 曹永红⁴, 苏群⁵, 王君^1,2, 杨军丽³

1.烟台中科先进材料与绿色化工产业技术研究院,山东烟台 264000;
2.烟台先进材料与绿色制造山东省实验室,山东烟台 264000;
3.中国科学院兰州化学物理研究所/中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室,兰州 730000;
4.陇南市经济林研究院花椒研究所,甘肃陇南 746000;
5.山东宝源生物科技股份有限公司,山东烟台 264000

收稿日期:2024-09-12 出版日期:2024-12-25 发布日期:2025-01-08
通讯作者: 杨军丽（1983-）,山西阳泉人,研究员,博士,主要从事天然产物化学研究,（电子信箱）yangjl@licp.cas.cn。
作者简介:李承钊（1997-）,男,河南新乡人,硕士,主要从事天然产物化学研究,（电话）19553582293（电子信箱）lichengzhao1997@163.com;王君（1991-）,山东济南人,助理研究员,博士,主要从事天然产物化学研究,（电话）17863829607（电子信箱）licpwangjun@163.com。
基金资助:
国家自然科学基金项目（32270419）; 山东省自然科学基金资助项目（ZR2021MB089; ZR2023MC040）; 烟台先进材料与绿色制造山东省实验室项目（E1R03SXM04）; 烟台开发区科技领军项目（2022RC005）; 陇南市科技计划项目（2022-S˙QKJ-03）

Zanthoxylum bungeanum essential oil composition analysis and its antifungal activity on four kinds of plant pathogenic fungi

LI Cheng-zhao^1,2, LIU Yu-jie^1,2, WANG Wei-feng^1,3, MENG Xian-hua^1,3, CAO Yong-hong⁴, SU Qun⁵, WANG Jun^1,2, YANG Jun-li³

1. Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai 264000, Shandong, China;
2. Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai,Yantai 264000, Shandong, China;
3. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences/Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou 730000, China;
4. Zanthoxylum Bungeanum Research Institute, Longnan Economic Forest Research Institute, Longnan 746000,Gansu, China;
5. Baoyuan Bio-Agri Science & Technology （Shandong） Co., Ltd., Yantai 264000,Shandong, China

Received:2024-09-12 Published:2024-12-25 Online:2025-01-08

摘要/Abstract

摘要： 采用索氏提取法制备花椒（Zanthoxylum bungeanum Maxim.）精油,利用菌丝生长速率法评价花椒精油对尖镰刀菌（Fusarium oxysporum Schltdl）、禾谷镰孢菌（Fusarium graminearum Schwabe）、盘长孢状刺盘孢（Colletotrichum gloeosporioides Penz. & Sacc.）和茄链格孢菌（Alternaria solani）菌丝生长的抑制活性,采用气相色谱-质谱联用仪（GC-MS）探究花椒精油的物质组成。结果表明,花椒精油对禾谷镰孢菌、尖镰刀菌、盘长孢状刺盘孢和茄链格孢菌均表现出一定的抗菌活性,半数效应浓度（EC₅₀）分别为1.552、2.243、6.064、7.078 mg/mL。GC-MS分析显示,花椒精油中的主要成分为萜类化合物,包括乙酸芳樟酯、芳樟醇、D-柠檬烯和桉叶油醇等。

关键词: 花椒（Zanthoxylum bungeanum Maxim.）精油, 成分分析, 植物病原真菌, 抗菌作用

Abstract: Zanthoxylum bungeanum essential oil was extracted using the Soxhlet method. The effects of Zanthoxylum bungeanum essential oil on Fusarium oxysporum, Fusarium graminearum, Colletotrichum gloeosporioides and Alternaria solani were evaluated by the mycelial growth rate method. The composition of Zanthoxylum bungeanum essential oil was analyzed using gas chromatography-mass spectrometry （GC-MS）. The results showed that Zanthoxylum bungeanum essential oil exhibited antifungal activity Fusarium graminiearum, Fusarium oxysporum, Colletotrichum gloeosporioides and Alternaria solani, with median effect concentration（EC₅₀） of 1.552, 2.243, 6.064, 7.078 mg/mL, respectively. GC-MS analysis revealed that the principal constituents of Zanthoxylum zanthoxylum essential oil were terpenoids including linalyl acetate, linalool, D-limonene and eucalyptol.

Key words: Zanthoxylum bungeanum essential oil, composition analysis, plant pathogenic fungi, antifungal activity

中图分类号:

李承钊, 刘玉杰, 王伟峰, 孟宪华, 曹永红, 苏群, 王君, 杨军丽. 花椒精油成分分析及其对四种植物病原真菌的抗菌作用[J]. 湖北农业科学, 2024, 63(12): 62-66.

LI Cheng-zhao, LIU Yu-jie, WANG Wei-feng, MENG Xian-hua, CAO Yong-hong, SU Qun, WANG Jun, YANG Jun-li. Zanthoxylum bungeanum essential oil composition analysis and its antifungal activity on four kinds of plant pathogenic fungi[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(12): 62-66.

参考文献

[1] KJER J,WRAY V,EDRADA-EBEL R,et al.Xanalteric acids I and II and related phenolic compounds from an endophytic Alternaria sp. isolated from the mangrove plant Sonneratia alba[J]. Journal of natural products,2009,72(11):2053-2057.
[2] 阙友雄,宋弦弦,许莉萍,等.植物与病原真菌互作机制研究进展[J]. 生物技术通讯,2009,20(2):282-285.
[3] DEAN R,VAN KAN J A L,PRETORIUS Z A,et al. The top 10 fungal pathogens in molecular plant pathology[J]. Mol Plant Pathol,2012,13(4):414-430.
[4] KUMAR S,SINGH R,KASHYAP P L,et al.Rapid detection and quantification of Alternaria solani in tomato[J]. Scientia horticulturae,2013,151:184-189.
[5] FOOLAD M R.Genome mapping and molecular breeding of tomato[J]. Int J Plant Genomics,2007,2007:64358.
[6] THOMMA B P.Alternaria spp.: From general saprophyte to specific parasite[J]. Mol Plant Pathol,2003,4(4):225-236.
[7] COVARELLI L,BECCARI G,PRODI A,et al.Fusarium species, chemotype characterisation and trichothecene contamination of durum and soft wheat in an area of central Italy[J]. J Sci Food Agric,2015,95(3):540-551.
[8] 苏培森,葛文扬,王宏伟,等.小麦-禾谷镰孢菌互作机制的研究进展[J]. 中国科学:生命科学,2021,51(11): 1493-1507.
[9] PRUSKY D.Pathogen quiescence in postharvest diseases[J]. Annu Rev Phytopathol,1996,34:413-434.
[10] MICHIELSE C B,REP M.Pathogen profile update: Fusarium oxysporum[J]. Mol Plant Pathol,2009,10(3):311-24.
[11] OLIVAIN C,ALABOUVETTE C.Process of tomato root colonization by a pathogenic strain of Fusarium oxysporum f. sp. lycopersici in comparison with a non-pathogenic strain[J]. New phytologist,1999,141(3):497-510.
[12] ZHANG D,BI W,KAI K,et al.Effect of chlorogenic acid on controlling kiwi fruit postharvest decay caused by Diaporthe sp.[J]. LWT--Food Sci Technol,2020,132:109805.
[13] WALIA S,SAHA S,TRIPATHI V,et al.Phytochemical biopesticides: Some recent developments[J]. Phytochemistry reviews,2017,16(5):989-1007.
[14] 张嘉祺,闵德栋,刘云国.植物精油抑菌机制及其在食用菌保鲜中的应用进展[J]. 中国果菜,2024,44(4):9-13.
[15] LI J,HUI T,WANG F,et al.Chinese red pepper (Zanthoxylum bungeanum Maxim.) leaf extract as natural antioxidants in salted silver carp(Hypophthalmichthys molitrix) in dorsal and ventral muscles during processing[J]. Food control,2015,56:9-17.
[16] 王峰,王海平.萃取方法对花椒精油的化学成份、生物活性研究[J]. 食品研究与开发,2017,38(21):65-68.
[17] 宋荣,曹亮,周佳民,等.花椒种质资源及其功能成分和生物学效应研究进展[J]. 湖南农业科学,2014 (17):23-26,29.
[18] PRIETO J A,PATIÑO O J,DELGADO W A,et al. Chemical composition, insecticidal, and antifungal activities of fruit essential oils of three Colombian Zanthoxylum species[J]. Chilean journal of agricultural research,2011,71(1):73-82.
[19] DUENAS-DEYA A,CASTANEDA-BAUTA R,MART-BAUTA R.Phytochemical study of the cuban endemic species Zanthoxylum pseudodumosum, a plant with potential antifungal activity[J]. Revista cubana de quimica,2020,32(3):406-419.
[20] GONG Y,HUANG Y,ZHOU L,et al.Chemical composition and antifungal activity of the fruit oil of Zanthoxylum bungeanum Maxim. (Rutaceae) from China[J]. Journal of essential oil research,2009,21(2):174-178.
[21] 杨滢滢,陈明,万春鹏,等.天然芳樟醇和柠檬醛对4种果蔬采后致病菌抑菌活性研究[J]. 生物灾害科学,2016,39(2):80-83.
[22] 陈红兵,高晋融,王仟莉,等.柠檬烯-6-醇新戊酸酯水乳剂的抑菌活性研究[J]. 广州化工,2021,49(7):70-71,101.
[23] FRATERNALE D,GIAMPERI L,RICCI D.Chemical composition and antifungal activity of essential oil obtained from in vitro plants of Thymus mastichina L[J]. Journal of essential oil research,2003,15(4):278-281.
[24] 李烨青,张昌朋,方楠,等.柠檬烯在农业病虫草害防控中的应用研究进展[J]. 农药学学报,2023,25(5):1004-1016.
[25] 肖瑞瑶,滕攀攀,钱玺丞,等.九叶青花椒挥发油GC-MS成分分析[J]. 云南化工,2023,50(6):78-81.

花椒精油成分分析及其对四种植物病原真菌的抗菌作用

Zanthoxylum bungeanum essential oil composition analysis and its antifungal activity on four kinds of plant pathogenic fungi

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄帅, 谭宏婧, 付尚可, 李晓慧, 王志新, 邢健, 吕囿成. 绿洲土壤盐分含量高光谱反演建模[J]. 湖北农业科学, 2024, 63(9): 196-203.
[2]	张萌, 徐建鹏, 周鹿扬, 王杰, 王状, 岳伟. 融合GF-6 WFV影像主成分分析特征的县域冬小麦种植面积提取[J]. 湖北农业科学, 2024, 63(8): 201-208.
[3]	马雯芳, 吴剑丽, 张秀丽, 蓝昌斌, 甘洁雪, 周华锋, 王剑. 壮药蛇尾草HPLC指纹图谱及抗氧化活性的谱效关系[J]. 湖北农业科学, 2024, 63(5): 84-90.
[4]	孙萌, 宁松瑞, 颜安, 杨利, 卢前成, 左筱筱, 李靖言, 范君. 生物菌肥下小麦苗期生长及土壤微生物的特征[J]. 湖北农业科学, 2024, 63(4): 24-29.
[5]	王井利, 余鹏程, 蔡福, 刘慧楠, 高天娇. 基于多时相RSEI的生态环境质量评价——以新民市为例[J]. 湖北农业科学, 2024, 63(4): 56-60.
[6]	付岩. 呼伦贝尔市乡村旅游在乡村文化振兴中的作用[J]. 湖北农业科学, 2024, 63(3): 8-13.
[7]	孟刚, 楚渠, 王瑞娴, 彭云武, 杨金宏, 刘强, 朱文东. 24个桑树品种桑叶营养和活性成分的测定与评价[J]. 湖北农业科学, 2024, 63(11): 72-78.
[8]	王丹青, 田英, 何军, 秦垦, 焦恩宁. 基于主成分分析法的不同产区及品种枸杞果实多元化质量评价[J]. 湖北农业科学, 2024, 63(10): 85-90.
[9]	吴楚鹏, 盛建东, 胡雨彤, 成志慧. 不同盐渍化棉田土壤肥力质量评价——以新疆沙雅县为例[J]. 湖北农业科学, 2023, 62(6): 33-38.
[10]	陈俊韬, 林锦耀. 基于地理探测器的耕地破碎化影响因素分析——以广州市为例[J]. 湖北农业科学, 2023, 62(5): 14-21.
[11]	邓喜明, 吕铎, 吴晓婷, 冯涛, 张美珍, 朱建强. 基于主成分分析的酒泉市农田土壤肥力评价[J]. 湖北农业科学, 2023, 62(3): 21-24.
[12]	刘纤纤, 李文莉, 梁晓莲, 周楚惠, 陈勇, 罗孔现, 刘兆祥. 广西不同产地红穿破石地上及地下部分HPLC指纹图谱研究[J]. 湖北农业科学, 2023, 62(12): 163-170,177.
[13]	丁岳炼, 王冰清, 陈杰, 万利鑫, 周建瑛, 王志云. 不同基质配比对短序润楠容器苗生长的影响[J]. 湖北农业科学, 2023, 62(11): 92-96.
[14]	王俊俊, 王玲丽. 运城市盐湖区景观格局演变及其驱动因素研究[J]. 湖北农业科学, 2022, 61(8): 81-86.
[15]	刘笑, 李林杰, 王昱涵, 王志萍. 基于多种分析模式建立壮药材火索藤的HPLC指纹图谱[J]. 湖北农业科学, 2022, 61(7): 103-107.