关键词: 半夏(Pinellia ternata), 大豆花叶病毒, 外壳蛋白, 原核表达, 多克隆抗体

Abstract: To establish a rapid detection method for soybean mosaic virus (SMV), the primary pathogen causing mosaic disease in Pinellia ternata, the coat protein gene of SMV was amplified by reverse transcription-polymerase chain reaction (RT-PCR), and its prokaryotic expression vector was constructed. The purified fusion-expressed protein was used as an antigen to immunize rabbits for the preparation of polyclonal antibodies. The results showed that the prepared antiserum exhibited a titer of 1∶102,400 as determined by enzyme-linked immunosorbent assay (ELISA), a Western blot detection limit of 300 ng for the antigen, and demonstrated strong specificity.

Key words: Pinellia ternata, soybean mosaic virus, coat protein, prokaryotic expression, polyclonal antibody