荞麦种间杂交群体基因定位解析

doi:10.14088/j.cnki.issn0439-8114.2025.05.026

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (5): 167-172.doi: 10.14088/j.cnki.issn0439-8114.2025.05.026

荞麦种间杂交群体基因定位解析

尹桂芳¹, 卢文洁¹, 隆文杰¹, 孙道旺¹, 黄春燕², 翟冰欣², 王莉花¹

1.云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,昆明 650205;
2.云南大学资源植物研究院,昆明 650500

收稿日期:2024-05-09 出版日期:2025-05-25 发布日期:2025-06-11
作者简介:尹桂芳（1981-）,女,云南宣威人,副研究员,硕士,主要从事荞麦生物技术育种研究,（电子信箱）1434651675@qq.com 共同第一作者,卢文洁（1976-）,女,云南建水人,副研究员,硕士,主要从事植物保护研究,（电子信箱）376495440@qq.com
基金资助:
云南省重大科技专项与重点研发计划项目(202202AE090020); 财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-07-C-2); 云南省农业基础研究联合专项（202101BD070001-122; 202301BD070001-039）

Genetic mapping analysis of interspecific hybrid population in buckwheat

YIN Gui-fang¹, LU Wen-jie¹, LONG Wen-jie¹, SUN Dao-wang¹, HUANG Chun-yan², ZHAI Bing-xin², WANG Li-hua¹

1. Biotechnology and Genetic Germplasm Resources Research Institute/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;
2. Institute of Resource Plants, Yunnan University, Kunming 650500, China

Received:2024-05-09 Published:2025-05-25 Online:2025-06-11

摘要/Abstract

摘要： 利用荞麦（Fagopyrum esculentum Moench.）栽培品种云荞1号（YQ1）与抗倒伏、抗病的地方野生种荞麦YZ56杂交构建F₂群体,基于农艺性状调查数据筛选出株高、单株粒重及叶枯病抗性等性状的极端表型个体,并采用CTAB法从荞麦叶片中提取DNA,构建极端表型DNA混池。结果表明,F₂群体的株高和单株粒重呈正态分布,且叶枯病发生率较低的个体在分布中占比较高。F₂群体的单株粒重平均值为17.8 g,高于亲本YZ56（13.1 g）和YQ1（16.7 g）,表现出明显的杂种优势,合理利用远缘种质资源进行杂交可以提高后代的产量。Pearson相关分析表明,株高、单株粒重和叶枯病率之间存在极显著相关性。通过混池测序结合BSA分析,在95%置信区间内成功定位到与株高相关的10个QTL区域、与单株粒重相关的7个候选QTL区域,以及与叶枯病抗性相关的6个候选QTL区域。

关键词: 荞麦（Fagopyrum esculentum Moench.）, 种间杂交群体, 基因组测序, 基因定位

Abstract: An F₂ population was constructed by crossing the cultivated buckwheat（Fagopyrum esculentum Moench.） variety Yunqiao 1 (YQ1) with the lodging-resistant and disease-resistant wild buckwheat YZ56,extreme phenotypic individuals for traits including plant height, grain weight per plant, and leaf blight resistance were screened based on agronomic trait investigation data,and DNA was extracted from buckwheat leaves using the CTAB method to construct extreme phenotypic DNA pools. The results demonstrated that plant height and grain weight per plant in the F₂ population showed normal distributions,with individuals exhibiting lower leaf blight incidence occupying a higher proportion in the distribution. The average grain weight per plant of the F2 population was 17.8 g, higher than that of the parental lines YZ56 (13.1 g) and YQ1 (16.7 g),indicating significant heterosis. Rational utilization of distant germplasm resources for hybridization could improve progeny yield.Pearson correlation analysis revealed highly significant correlations among plant height, grain weight per plant, and leaf blight incidence rate. Pooled sequencing combined with bulked segregant analysis (BSA) successfully identified 10 QTL regions associated with plant height at a 95% confidence interval,7 candidate QTL regions related to grain weight per plant,and 6 candidate QTL regions linked to leaf blight resistance.

Key words: buckwheat（Fagopyrum esculentum Moench.）, interspecific hybrid population, genome sequencing, gene mapping

中图分类号:

S334

尹桂芳, 卢文洁, 隆文杰, 孙道旺, 黄春燕, 翟冰欣, 王莉花. 荞麦种间杂交群体基因定位解析[J]. 湖北农业科学, 2025, 64(5): 167-172.

YIN Gui-fang, LU Wen-jie, LONG Wen-jie, SUN Dao-wang, HUANG Chun-yan, ZHAI Bing-xin, WANG Li-hua. Genetic mapping analysis of interspecific hybrid population in buckwheat[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 167-172.

参考文献

[1] 唐宇,邵继荣,周美亮.中国荞麦属植物分类学的修订[J].植物遗传资源学报,2019,20(3):646-653.
[2] 范昱,丁梦琦,张凯旋,等.荞麦种质资源概况[J].植物遗传资源学报,2019,20(4):813-828.
[3] 范昱,丁梦琦,张凯旋,等.中国野生荞麦种质资源概况与利用进展[J].植物遗传资源学报,2020,21(6):1395-1406.
[4] 内蒙古农业科学院,中国农业科学院作物品种资源研究所.中国荞麦品种资源目录(第一辑)[M].北京:中国农业出版社,1986.
[5] 中国农业科学院作物品种资源研究所.中国荞麦品种资源目录(第二辑)[M].北京:中国农业出版社,1996.
[6] 赵建栋,李秀莲,陈稳良,等. 我国荞麦育种成就、问题及对策[J].种子,2017,36(4):67-71.
[7] 王莉花,王艳青,卢文洁,等.云荞1号的选育及高产栽培技术[J].山西农业科学,2012,40(8):829-832.
[8] TAKESHIMA R, OGISO-TANAKA E, YASUI Y, et al.Targeted amplicon sequencing + next-generation sequencing-based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)[J].BMC plant biology,2021, 21(1):18.
[9] DUAN Y, YIN G, HE R,et al. Identification of candidate genes for easily-shelled traits in Tartary buckwheat based on BSA-Seq and RNA-Seq methods[J].Euphytica, 2022,218, 91:42.
[10] 汪灿,阮仁武,袁晓辉,等.荞麦茎秆解剖结构和木质素代谢及其与抗倒性的关系[J].作物学报,2014,40(10):1846-1856.
[11] 汪灿,阮仁武,袁晓辉,等.不同荞麦品种抗倒伏能力与根系及茎秆性状的关系[J].西南大学学报(自然科学版),2015,37(1): 65-71.
[12] 李英双,胡丹,聂蛟,等.甜荞株高和茎粗的遗传分析[J].作物学报,2018,44(8):1185-1195.
[13] 胡丹. 甜荞茎秆重心高度和抗折力的遗传分析[J].作物杂志, 2022 (4): 83-89.
[14] SHEN Q, PENG X X, HE F,et al.First report of Nigrospora osmanthi causing leaf spot on tartary buckwheat in China[J].Plant disease, 2021, 105: 1227.
[15] 卢文洁,罗志明,李春花,等.云南省荞麦叶枯病病原菌鉴定及其生物学特性[J].植物保护学报,2019,46(5):1065-1072.
[16] 李月,石桃雄,黄凯丰,等.苦荞生态因子及农艺性状与产量的相关分析[J].西南农业学报,2013,26(1):35-41.
[17] 陈庆富,陈其饺,石桃雄,等. 苦荞厚果壳性状的遗传及其与产量因素的相关性研究[J].作物杂志,2015 (2):27-31.
[18] 陈稳良, 李秀莲, 史兴海, 等.甜荞产量性状综合评价及AMMI分析[J].核农学报, 2023, 37(1): 60-68
[19] ZHANG L, LI X, MA B, et al.The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance[J].Molecular plant,2017, 10(9):1224-1237.
[20] HE Q, MA D, LI W, et al.High-quality Fagopyrum esculentum genome provides insights into the flavonoid accumulation among different tissues and self-incompatibility[J].Journal of integrative plant biology,2023,6: 1423-1441.

荞麦种间杂交群体基因定位解析

Genetic mapping analysis of interspecific hybrid population in buckwheat

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