基于代谢组学分析根瘤菌调节因子nolR对大豆根系代谢的影响

doi:10.14088/j.cnki.issn0439-8114.2025.08.033

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (8): 219-225.doi: 10.14088/j.cnki.issn0439-8114.2025.08.033

基于代谢组学分析根瘤菌调节因子nolR对大豆根系代谢的影响

毕宇^a, 朱加楠^a, 甄涛^a, 田爽^a, 吴皓琼^a, 于德水^a,b, 高冬元^c

黑龙江省科学院,a.微生物研究所; b.机关党委办公室; c.石油化学研究院,哈尔滨 150040

收稿日期:2024-11-20 出版日期:2025-08-25 发布日期:2025-09-12
通讯作者: 于德水（1969-）,男,吉林磐石人,研究员,硕士,主要从事植物与微生物互作研究,（电话）18646077835（电子信箱）yds_2381@126.com;高冬元（1969-）,女,黑龙江哈尔滨人,副研究员,主要从事科技管理研究,（电话）13091879618（电子信箱）1322539742@qq.com。
作者简介:毕宇（2000-）,女,黑龙江黑河人,在读硕士研究生,研究方向为植物与微生物互作,（电话）18724331889（电子信箱）1272084748@qq.com;
基金资助:
黑龙江省属科研院所科研业务费项目（2023SSKY004-04）

Metabolomics analysis of the effects of the rhizobial regulator nolR on root metabolism in soybean

BI Yu^a, ZHU Jia-nan^a, ZHEN Tao^a, TIAN Shuang^a, WU Hao-qiong^a, YU De-shui^a,b, GAO Dong-yuan^c

a.Institute of Microbiology; b.Office of the Party Committee; c.Institute of Petrochemistry, Heilongjiang Academy of Sciences, Harbin 150040, China

Received:2024-11-20 Published:2025-08-25 Online:2025-09-12

摘要/Abstract

摘要： 采用代谢组学技术分析大豆[Glycine max (L.) Merr.]黑科60接种费氏中华根瘤菌（Sinorhizobium fredii）HH103（以下简称根瘤菌HH103）和突变体HH103ΔnolR后根系代谢物差异。结果表明,在根瘤菌HH103处理与突变体HH103ΔnolR处理样本间共鉴定到9 907个差异代谢物,其中,4 202个代谢物含量上调,5 705个代谢物含量下调。差异代谢物中脂质和类脂分子（Lipids and lipid-like molecules）、苯丙类和聚酮类（Phenylpropanoids and polyketides）的占比较高。接种根瘤菌后差异代谢物含量上调幅度最大的为锦葵色素苷（Malvin）,下调幅度最大的是酸浆苦味素F（Physalin_F）。差异代谢物显著富集在半乳糖代谢（Galactose metabolism）、赖氨酸生物合成（Lysine biosynthesis）、卟啉代谢（Porphyrin metabolism）、异喹啉生物碱的生物合成（Isoquinoline alkaloid biosynthesis）、丙酸盐代谢（Propanoate metabolism）等通路。在差异代谢物中,类黄酮化合物的含量差异比较显著,nolR基因的缺失促进了大豆中类黄酮化合物的代谢。

关键词: 大豆[Glycine max (L.) Merr.], 根瘤菌, 调节因子nolR, 代谢组学

Abstract: Metabolomics technology was employed to analyze differences in root metabolites of soybean[Glycine max (L.) Merr.] cultivar Heike 60 after inoculation with Sinorhizobium fredii HH103(hereinafter referred to as rhizobium HH103) and its mutant HH103ΔnolR. The results showed that a total of 9 907 differential metabolites were identified between samples from the rhizobium HH103 treatment and the mutant HH103ΔnolR treatment, among which the content of 4 202 metabolites was upregulated and that of 5 705 metabolites was downregulated. Lipids and lipid-like molecules, as well as phenylpropanoids and polyketides, accounted for a relatively high proportion among the differential metabolites. Malvin exhibited the largest increase in differential metabolite content after inoculation with the rhizobium, while Physalin_F showed the largest decrease. The differential metabolites were significantly enriched in pathways such as galactose metabolism, lysine biosynthesis, porphyrin metabolism, isoquinoline alkaloid biosynthesis, and propanoate metabolism. Among the differential metabolites, the content differences in flavonoid compounds were relatively significant, and the deletion of the nolR gene promoted the metabolism of flavonoid compounds in soybean.

Key words: soybean[Glycine max (L.) Merr.], rhizobium, regulator nolR, metabolomics

中图分类号:

S565

毕宇, 朱加楠, 甄涛, 田爽, 吴皓琼, 于德水, 高冬元. 基于代谢组学分析根瘤菌调节因子nolR对大豆根系代谢的影响[J]. 湖北农业科学, 2025, 64(8): 219-225.

BI Yu, ZHU Jia-nan, ZHEN Tao, TIAN Shuang, WU Hao-qiong, YU De-shui, GAO Dong-yuan. Metabolomics analysis of the effects of the rhizobial regulator nolR on root metabolism in soybean[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(8): 219-225.

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基于代谢组学分析根瘤菌调节因子nolR对大豆根系代谢的影响

Metabolomics analysis of the effects of the rhizobial regulator nolR on root metabolism in soybean

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