木本植物遗传转化体系构建相关研究进展

doi:10.14088/j.cnki.issn0439-8114.2025.10.017

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (10): 110-118.doi: 10.14088/j.cnki.issn0439-8114.2025.10.017

木本植物遗传转化体系构建相关研究进展

梁海雪¹, 曾娟^1,2, 宋升奕¹, 雷子晨¹, 罗诗玉¹, 曹凌婧¹, 刘湘丹^1,2,3,4

1.湖南中医药大学药学院,长沙 410208;
2.湘产大宗道地药材种质资源及规范化种植重点研究室,长沙 410208;
3.湖南省普通高等学校中药现代化研究重点实验室,长沙 410208;
4.湖南省中药饮片标准化及功能工程技术研究中心,长沙 410208

收稿日期:2025-04-09 出版日期:2025-10-25 发布日期:2025-11-14
通讯作者: 刘湘丹(1981-),女,湖南涟源人,教授,博士,主要从事中药资源与品质评价的研究工作,(电话)0731-88458233(电子信箱)paeonia@hnucm.edu.cn。
作者简介:梁海雪(2003-),女,海南海口人,2021级在读本科生,中药资源与开发专业,(电话)0731-88458233(电子信箱)2652573439@qq.com。
基金资助:
国家自然科学基金项目(82373992); 湖南中医药大学本科生科研创新基金项目(2023BKS102); 湖南省中药材产业技术体系项目(HARS-11); 2020年湖南省一流专业建设点：中药资源与开发; 湖南中医药大学重点学科中药学科(校行发规字〔2023〕2号)

Research progress on the construction of woody plant genetic transformation system

LIANG Hai-xue¹, ZENG Juan^1,2, SONG Sheng-yi¹, LEI Zi-chen¹, LUO Shi-yu¹, CAO Ling-jing¹, LIU Xiang-dan^1,2,3,4

1. School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208,China;
2. Key Laboratory of Germplasm Resources and Standardized Planting of Genuine Medicinal Materials Produced in Hunan, Changsha 410208,China;
3. Hunan Provincial Key Laboratory of Modernization of Traditional Chinese Medicine, Changsha 410208,China;
4. Hunan Traditional Chinese Medicine Pieces Standardization and Functional Engineering Technology Research Center, Changsha 410208,China

Received:2025-04-09 Published:2025-10-25 Online:2025-11-14

摘要/Abstract

摘要： 介绍了木本植物的遗传转化方法,并比较了不同遗传转化方法的优缺点。阐述了木本植物遗传转化的影响因素以及不同遗传转化方法在木本植物中的应用现状。对现有的研究进行总结,指出了现阶段研究的不足之处,提出解决方法以及未来的研究方向,以期为后续木本植物遗传转化体系的构建提供参考。

关键词: 木本植物, 遗传转化体系, 农杆菌介导法, 基因枪法

Abstract: This review introduced the genetic transformation methods of woody plants and compared the advantages and disadvantages of different genetic transformation methods. The influencing factors of genetic transformation of woody plants were elaborated, and the application status of different genetic transformation methods in woody plants was introduced. The existing research was summarized, the shortcomings of the current research were pointed out, and the solutions and future research directions and development trends were proposed, in order to provide research ideas and references for the construction of subsequent genetic transformation systems of woody plants.

Key words: woody plant, genetic transformation system, agrobacterium-mediated method, gene marksmanship

中图分类号:

Q785

梁海雪, 曾娟, 宋升奕, 雷子晨, 罗诗玉, 曹凌婧, 刘湘丹. 木本植物遗传转化体系构建相关研究进展[J]. 湖北农业科学, 2025, 64(10): 110-118.

LIANG Hai-xue, ZENG Juan, SONG Sheng-yi, LEI Zi-chen, LUO Shi-yu, CAO Ling-jing, LIU Xiang-dan. Research progress on the construction of woody plant genetic transformation system[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 110-118.

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木本植物遗传转化体系构建相关研究进展

Research progress on the construction of woody plant genetic transformation system

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