岩黄连蜡质代谢物的GC-MS分析

doi:10.14088/j.cnki.issn0439-8114.2026.02.026

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 176-182.doi: 10.14088/j.cnki.issn0439-8114.2026.02.026

岩黄连蜡质代谢物的GC-MS分析

张奕萱^1,2, 黄彦棋^1,2, 雷明¹, 韦莹¹, 肖冬², 李翠¹

1.广西壮族自治区药用植物园/国家中医药传承创新中心/广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023;
2.广西大学农学院/广西农业环境与农产品安全重点实验室/植物科学国家级实验教学示范中心,南宁 530004

收稿日期:2025-09-28 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 李翠（1982-）,女,黑龙江依安人,主任技师,硕士,主要从事药用植物资源保育研究,（电话）13557611106（电子信箱）licuicui941@163.com。
作者简介:张奕萱（1999-）,女,广西博白人,硕士,主要从事药用植物资源保育研究,（电话）17891197621（电子信箱）468945225@qq.com。
基金资助:
中央引导地方科技发展资金项目（桂科ZY24212031）; 广西重点研发计划（桂科AB23026092）; 广西自然科学基金面上项目（2025GXNSFAA069814; 2025GXNSFBA069023; 2023GXNSFAA026509）; 国家自然科学基金项目（32460105）; 广西岐黄学者培养项目（GXQH202402）; 广西壮族自治区中医药管理局自筹经费科研课题（GXZYA20250004）; 广西药用资源保护与遗传改良重点实验室项目（KL2025ZZ02; KL2023KF04）

GC-MS analysis of the waxy metabolites of Corydalis saxicola

ZHANG Yi-xuan^1,2, HUANG Yan-qi^1,2, LEI Ming¹, WEI Ying¹, XIAO Dong², LI Cui¹

1. Guangxi Botanical Garden of Medicinal Plants/National TCM Inheritance and Innovation Center/ Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Zhuang Autonomous Region Engineering Research Center for the Intelligent Creation of Traditional Chinese Medicine Resources, Nanning 530023, China;
2. College of Agriculture, Guangxi University/Guangxi Key Laboratory of Agricultural Environment and Agricultural Product Safety/National Experimental Teaching Demonstration Center of Plant Science, Nanning 530004, China

Received:2025-09-28 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 以岩黄连（Corydalis saxicola）叶片为研究对象,采用气相色谱-质谱联用（GC-MS）技术对岩黄连蜡质代谢物进行分析和鉴定。结果表明,5个产区的岩黄连蜡质代谢物组成存在显著差异。共鉴定到包括长链烷烃（如正二十九烷、正五十四烷）、酮类、甾醇（如γ-谷甾醇）以及生物碱类（如四氢小檗碱）在内的多种关键差异代谢物。通路富集分析显示,差异代谢物显著富集于“异喹啉类生物碱生物合成”及“各类次级代谢物的生物合成”等代谢途径。此外,蜡质中还发现了鬼臼毒素等具有生物活性的防御性化合物。研究结果为岩黄连质量评价和相关产品研发提供了科学依据。

关键词: 岩黄连（Corydalis saxicola）, 差异代谢物, 蜡质, 气相色谱-质谱联用（GC-MS）

Abstract: Taking the leaves of Corydalis saxicola as the research object, the waxy metabolites of Corydalis saxicola were analyzed and identified by gas chromatography-mass spectrography（GC-MS）. The results showed that there were significant differences in the composition of waxy metabolites of Corydalis saxicola in the five producing areas. A total of several key differential metabolites were identified, including long-chain alkanes (such as n-29-alkane and n-54-alkane), ketones, sterols (such as γ-oryzanol), and alkaloids (such as tetrahydroberberine). Pathway enrichment analysis revealed that the differential metabolites were significantly enriched in metabolic pathways such as "isoquinoline alkaloid biosynthesis" and "biosynthesis of various secondary metabolites". In addition, bioactive defensive compounds such as podophyllotoxin were also found in the wax. The results provided a scientific basis for quality evaluation and related product development of Corydalis saxicola.

Key words: Corydalis saxicola, differential metabolites, waxy substance, gas chromatography-mass spectrography（GC-MS）

中图分类号:

O657.63

张奕萱, 黄彦棋, 雷明, 韦莹, 肖冬, 李翠. 岩黄连蜡质代谢物的GC-MS分析[J]. 湖北农业科学, 2026, 65(2): 176-182.

ZHANG Yi-xuan, HUANG Yan-qi, LEI Ming, WEI Ying, XIAO Dong, LI Cui. GC-MS analysis of the waxy metabolites of Corydalis saxicola[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 176-182.

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岩黄连蜡质代谢物的GC-MS分析

GC-MS analysis of the waxy metabolites of Corydalis saxicola

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