两个不同品种肉兔TLR4基因多态性研究

doi:10.14088/j.cnki.issn0439-8114.2018.11.025

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (11): 100-102.doi: 10.14088/j.cnki.issn0439-8114.2018.11.025

两个不同品种肉兔TLR4基因多态性研究

张翔宇¹, 李金良¹, 李旭廷¹, 李思聪¹, 张敏¹, 王吴燕², 杨锐¹

1.四川省畜牧科学研究院/动物遗传育种四川省重点实验室,成都 610066;
2.成都鼎鑫兔业发展有限责任公司,成都 610100

收稿日期:2018-02-23 出版日期:2018-06-10 发布日期:2019-12-19
通讯作者: 杨锐,助理研究员,主要从事肉兔的饲养管理研究,（电子信箱）236669091@qq.com。
作者简介:张翔宇（1981-）,男,四川西昌人,副研究员,博士,主要从事肉兔的遗传育种与饲养管理研究,（电话）15198130565（电子信箱）zhangxiangyu757@163.com
基金资助:
四川省畜禽育种攻关项目（2016NYZ0046）

Single Nucleotide Polymorphisms of Toll-Like Receptor 4 Gene in the Two Breeds Rabbit

ZHANG Xiang-yu¹, LI Jin-liang¹, LI Xu-ting¹, LI Si-chong¹, ZHANG Min¹, WANG Wu-yan², YANG Rui¹

1.Sichuan Key Laboratory of Animal Heredity and Breeding,Sichuan Animal Science Academy,Chengdu 610066,China;
2.Ding Xin Rabbit Breeding Co.,Ltd.,Chengdu 610100,China

Received:2018-02-23 Online:2018-06-10 Published:2019-12-19

摘要/Abstract

摘要： TLR4能识别内毒素脂多糖（LPS）,在介导革兰氏阴性菌及其LPS的宿主反应中发挥重要作用,是研究肉兔抗病力的重要候选基因。为了研究TLR4在不同肉兔品种的突变情况,设计引物对2个不同肉兔品种的12个个体的TLR4部分编码区进行序列扩增,并比较其序列。结果表明,共检测到3个SNP位点,均为非同义突变。2个非同义位点（G628T和C653A）位于预测蛋白的亮氨酸富集区（LRR）区域。LRR区域发生的非同义突变,尤其是亮氨酸的改变和氨基酸极性的改变可能会影响TLR4对病原的识别,这些SNP位点将有助于肉兔抗病分子标记的研究。

关键词: 肉兔, TLR4基因, 单核苷酸突变, 非同义突变, 抗病力

Abstract: TLR4 could detect endotoxin by identifying lipopolysaccharide （LPS） and play a key role in host defense mediated by Gram-negative bacteria（LPS）,therefore, TLR4 is a significant candidate gene for rabbit diseases resistance research. To identify nucleotide polymorphisms between two different breeds we designed primer pairs that amplified fragments of coding sequence（CDS） of 12 individuals, and compared the sequences. Comparative sequence analysis indicated that 3 SNPs were identified, all were non-synonymous SNPs. Two nonsynonymous SNPs（G628T and A653C） were located in the LRR domains of the predicted protein. The nonsynonymous SNPs in the LRR domains, especially related to leucine residues, may dramatically alter the ability to identified extracellular pathogens. The identification of these polymorphisms reported here increases the resource of genetic markers useful for association analyses with disease resistance and provides an important theoretical basis for disease resistant breeding of meat rabbits.

Key words: meat rabbits, TLR4 gene, SNP, nonsynonymous SNPs, disease resistance

中图分类号:

S829.1

张翔宇, 李金良, 李旭廷, 李思聪, 张敏, 王吴燕, 杨锐. 两个不同品种肉兔TLR4基因多态性研究[J]. 湖北农业科学, 2018, 57(11): 100-102.

ZHANG Xiang-yu, LI Jin-liang, LI Xu-ting, LI Si-chong, ZHANG Min, WANG Wu-yan, YANG Rui. Single Nucleotide Polymorphisms of Toll-Like Receptor 4 Gene in the Two Breeds Rabbit[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(11): 100-102.

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两个不同品种肉兔TLR4基因多态性研究

Single Nucleotide Polymorphisms of Toll-Like Receptor 4 Gene in the Two Breeds Rabbit

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