三角形五肽重复蛋白研究进展

doi:10.14088/j.cnki.issn0439-8114.2018.23.004

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (23): 19-23.doi: 10.14088/j.cnki.issn0439-8114.2018.23.004

三角形五肽重复蛋白研究进展

黄俊然, 黄文超

武汉大学生命科学学院/杂交水稻国家重点实验室,武汉 430070

收稿日期:2018-06-13 出版日期:2018-12-10 发布日期:2019-12-18
通讯作者: 黄文超,男,副研究员,博士,（电子信箱）wenchaoh@whu.edu.cn。
作者简介:黄俊然（1992-）,男,广西柳州人,在读硕士研究生,研究方向为植物发育遗传学,（电话）17671654305（电子信箱）JunranHuang@whu.edu.cn
基金资助:
国家自然科学基金项目（31771746）; 国家水稻产业技术体系项目（CARS-01-07）; 杂交水稻国家重点实验室（武汉大学）开放课题基金资助项目

Research Progress on the Pentatricopeptide-repeat Protein

HUANG Jun-ran, HUANG Wen-chao

State Key Laboratory of Hybrid Rice/College of Life Sciences,Wuhan University,Wuhan 430070,China

Received:2018-06-13 Online:2018-12-10 Published:2019-12-18

摘要/Abstract

摘要： 三角形五肽重复（PPR）蛋白是植物中一个庞大的蛋白家族,能够从RNA剪切、编辑、降解和翻译等多个方面影响细胞器中RNA的新陈代谢,PPR蛋白由连续的PPR基序构成,蛋白呈右手螺旋结构,具有与RNA单链结合的能力。随着研究的深入,人工设计合成PPR蛋白技术已经成熟,PPR蛋白与RNA结合的规律已较为清晰,人工PPR蛋白有望被开发成为细胞器中介导RNA调控的新型生物技术。

关键词: 三角形五肽重复蛋白, RNA单链结合, 细胞器RNA调控

Abstract: Pentatricopeptide-repeat,PPR protein is a huge protein family in plants. They can affect the metabolism of RNA in organelles,such as RNA splicing,editing,degradation and translation. PPR protein is composed of continuous PPR motifs. The protein shows a right hand spiral structure and has the ability to bind to the single strand of RNA. With the development of research,artificial design of PPR protein has matured. The rules of interaction between PPR protein and RNA are clearer. It is expected to be developed into a new biological technology for the regulation of RNA in organelles.

Key words: pentatricopeptide-repeat protein, RNA single strand binding, organelle RNA regulation

中图分类号:

黄俊然, 黄文超. 三角形五肽重复蛋白研究进展[J]. 湖北农业科学, 2018, 57(23): 19-23.

HUANG Jun-ran, HUANG Wen-chao. Research Progress on the Pentatricopeptide-repeat Protein[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(23): 19-23.

参考文献

[1] SCHMITZ-LINNEWEBER C,SMALL I.Pentatricopeptide repeat proteins:a socket set for organelle gene expression[J].Trends in Plant Science,2008,13(12):663-700.
[2] NAKAMURA T,YAGI Y,KOBAYASHI K.Mechanistic insight into pentatricopeptide repeat proteins as sequence-specific RNA-binding proteins for organellar RNAs in plants[J].Plant & Cell Physiology,2012,53(7):1171-1179.
[3] BARKAN A,ROJAS M,FUJII S,et al.A combinatorial amino acid code for RNA recognition by pentatricopeptide repeat proteins[J]. PLoS Genetics,2012,8(8):e1002910.
[4] LURIN C,ANDRES C,AUBOURG S,et al.Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis[J].The Plant Cell, 2004,16(8):2089-2103.
[5] BAN T,KE J,CHEN R,et al.Structure of a PLS-class pentatricopeptide repeat protein provides insights into mechanism of RNA recognition[J].The Journal of Biological Chemistry,2013, 288(44):31540-31548.
[6] YIN P,LI Q,YAN C,et al.Structural basis for the modular recognition of single-stranded RNA by PPR proeins[J].Nature,2013,504(7478):168.
[7] HOWARD MJ,LIM W H,Fierke CA,et al.Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5′ processing[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(40):16149-16154.
[8] CHUJO T,OHIRA T,SAKAGUCHI Y,et al.LRPPRC/SLIRP suppresses PNPase-mediated mRNA decay and promotes polyadenylation in human mitochondria[J].Nucleic Acids Research,2012,40(16):8033-8047.
[9] SPAHR H,ROZANSKA A,LI X,et al.SLIRP stabilizes LRPPRC via an RRM-PPR protein interface[J].Nucleic Acids Research,2016,44(14):6868-6882.
[10] SIIRA S J,SPAHR H,SHEARWOOD A J,et al.LRPPRC-mediated folding of the mitochondrial transcriptome[J].Nature Communications,2017,8(1):1532.
[11] PFALZ J,BAYRAKTAR OA,PRIKRYL J,et al.Site-specific binding of a PPR protein defines and stabilizes 5' and 3' mRNA termini in chloroplasts[J].The EMBO Journal,2009, 28(14):2042-2052.
[12] BREHME N,BAYER-CSASZAR E,GLASS F,et al.The DYW subgroup PPR protein MEF35 Targets RNA editing sites in the mitochondrial rpl16,nad4 and cob mRNAs in Arabidopsis thaliana[J].PloS One,2015,10(10):e0140680.
[13] ICHINOSE M,SUGITA C,YAGI Y,et al.Two DYW subclass PPR proteins are involved in RNA editing of ccmFc and atp9 transcripts in the moss Physcomitrella patens: first complete set of PPR editing factors in plant mitochondria[J].Plant & Cell Physiology,2013,54(11):1907-1916.
[14] KHROUCHTCHOVA A,MONDE R A,BARKAN A.A short PPR protein required for the splicing of specific group II introns in angiosperm chloroplasts[J].Rna,2012,18(6):1197-1209.
[15] DE LONGEVIALLE AF,HENDRICKSON L,TAYLOR N L,et al.The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana[J].The Plant Journal:for Cell and Molecular Biology,2008,56(1):157-168.
[16] SHEN C,ZHANG D,GUAN Z,et al.Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins[J].Nature Communications,2016,7:11285.
[17] COQUILLE S,FILIPOVSKA A,CHIA T,et al.An artificial PPR scaffold for programmable RNA recognition[J].Nature Communications,2014,5:5729.
[18] MIRANDA RG,MCDERMOTT JJ,BARKAN A.RNA-binding specificity landscapes of designer pentatricopeptide repeat proteins elucidate principles of PPR-RNA interactions[J].Nucleic Acids Research,2017,46(5):2613-2623.
[19] CHEN T H,TANIMOTO A,SHKRIABAI N,et al.Use of chemical modification and mass spectrometry to identify substrate-contacting sites in proteinaceous RNase P,a tRNA processing enzyme[J].Nucleic Acids Research,2016,44(11):5344-5355.
[20] LIU S,MELONEK J,BOYKIN L M,et al.PPR-SMRs:Ancient proteins with enigmatic functions[J].RNA Biology,2013,10(9):1501-1510.
[21] PRIKRYL J,ROJAS M,SCHUSTER G,et al.Mechanism of RNA stabilization and translational activation by a pentatricopeptide repeat protein[J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(1):415-420.
[22] HU J,WANG K,HUANG W,et al.The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162[J].The Plant Cell,2012,24(1):109-122.
[23] QIN X,HUANG Q,XIAO H,et al.The rice DUF1620-containing and WD40-like repeat protein is required for the assembly of the restoration of fertility complex[J].The New Phytologist,2016,210(3):934-945.
[24] HUANG W,YU C,HU J,et al.Pentatricopeptide-repeat family protein RF6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(48):14984-14989.

三角形五肽重复蛋白研究进展

Research Progress on the Pentatricopeptide-repeat Protein

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价