高絮凝活性放线菌的鉴定和培养基组分的初步优化

doi:10.14088/j.cnki.issn0439-8114.2021.24.022

湖北农业科学 ›› 2021, Vol. 60 ›› Issue (24): 94-97.doi: 10.14088/j.cnki.issn0439-8114.2021.24.022

高絮凝活性放线菌的鉴定和培养基组分的初步优化

田宇曦, 陈凌, 闵勇, 杨自文

湖北省生物农药工程研究中心,武汉 430064

收稿日期:2021-10-12 出版日期:2021-12-25 发布日期:2022-01-04
通讯作者: 杨自文（1962-）,男,研究员,主要从事生物农药科学的研究工作,（电话）027-59101999（电子信箱）ziwen.yang@nberc.com。
作者简介:田宇曦（1985-）,女,湖北汉川人,助理研究员,硕士,主要从事微生物农药相关产品的开发工作,（电话）15926331448（电子信箱）332421996@qq.com;
基金资助:
湖北省池塘绿色生态养殖技术创新行动; 湖北省中央引导地方科技发展专项（2018ZYYD015）

Screening of high-efficiency bioflocculant-producing actinobacteria and preliminary optimization of medium composition

TIAN Yu-xi, CHEN Ling, MIN Yong, YANG Zi-wen

Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China

Received:2021-10-12 Online:2021-12-25 Published:2022-01-04

摘要/Abstract

摘要： 为了开发用于农村污水治理的高絮凝活性菌株,拟对高岭土有91.9%絮凝率的放线菌ws75382进行16S rDNA鉴定,并利用单因素试验和正交试验对其培养基组分进行优化。经过16S rDNA鉴定表明,放线菌ws75382与Streptomyces cyslabdanicus相似性最高,放线菌ws75382初始培养基组分多,碳氮源组分浓度偏高,优化获得的培养基组分氮源减少了1个成分,并且碳、氮源浓度分别降低了40%和30%,优化后的培养基组分为大豆粉15 g/L、葡萄糖14 g/L、氯化钠8 g/L和碳酸钙3 g/L,在此培养基组分下,ws75382絮凝率可以达100%。

关键词: 农村污水治理, 放线菌, 絮凝, 菌株鉴定, 培养基组分

Abstract: In order to develop a strain with high flocculation activity for rural sewage treatment, actinomycete ws75382 with the flocculation rate 91.9% of kaolin was identified by 16S rDNA, and its medium composition were optimized by single factor test and orthogonal test. 16S rDNA identification showed that actinomycetes ws75382 had the highest similarity with Streptomyces cyslabdanicus. The initial culture medium of actinomycetes ws75382 had many components, and the concentration of carbon and nitrogen sources was higher. The optimized medium composition reduced the nitrogen sources by one component, and the concentrations of carbon and nitrogen sources reduced 40% and 30%, respectively. The optimized culture medium components were soybean powder 15 g/L, glucose 14 g/L, sodium chloride 8 g/L and calcium carbonate 3 g/L, and under the conditions the flocculation rate of kaolin was nealy 100%.

Key words: rural sewage treatment, actinomycetes, flocculation, strain identification, medium composition

中图分类号:

X703

田宇曦, 陈凌, 闵勇, 杨自文. 高絮凝活性放线菌的鉴定和培养基组分的初步优化[J]. 湖北农业科学, 2021, 60(24): 94-97.

TIAN Yu-xi, CHEN Ling, MIN Yong, YANG Zi-wen. Screening of high-efficiency bioflocculant-producing actinobacteria and preliminary optimization of medium composition[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(24): 94-97.

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高絮凝活性放线菌的鉴定和培养基组分的初步优化

Screening of high-efficiency bioflocculant-producing actinobacteria and preliminary optimization of medium composition

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