阿耶波多氏芽孢杆菌对盐胁迫下绿豆的促生效果

doi:10.14088/j.cnki.issn0439-8114.2024.10.002

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (10): 8-13.doi: 10.14088/j.cnki.issn0439-8114.2024.10.002

阿耶波多氏芽孢杆菌对盐胁迫下绿豆的促生效果

刘彩婷^1,2a, 叶颖婷^1,2a, 范铭丰³, 陈晓逸^1,2b, 刘希华^1,2b

1.福建省资源环境监测与可持续经营利用重点实验室,福建三明 365004;
2.三明学院,a.海峡理工学院;b.资源与化工学院,福建三明 365004;
3.三明市土肥站,福建三明 365000

收稿日期:2024-05-20 出版日期:2024-10-25 发布日期:2024-11-05
通讯作者: 刘希华（1978-）,男,福建闽清人,教授,主要从事抗逆境植物选育,（电子信箱）xihua7808@163.com。
作者简介:刘彩婷（2002-）,女,福建福清人,在读本科生,专业方向为生物技术与生物育种,（电子信箱）1597324463@qq.com。
基金资助:
福建省自然基金面上项目（2020J01375）; 国家级大学生创新创业项目（202311311002）; 福建省大学生创新创业项目（S202311311071）

Growth-promoting effect of Bacillus aryabhattai on mung bean under salt stress

LIU Cai-ting^1,2a, YE Ying-ting^1,2a, FAN Ming-feng³, CHEN Xiao-yi^1,2b, LIU Xi-hua^1,2b

1. Fujian Provincial Key Laboratory of Resources, Environment Monitoring & Sustainable Management, Utilization, Sanming 365004, Fujian, China;
2a. Strait Institute of Technology;2b. School of Resources, Chemical Engineering, Sanming University, Sanming 365004, Fujian, China;
3. Sanming Soil, Fertilizer Station, Sanming 365000, Fujian, China

Received:2024-05-20 Published:2024-10-25 Online:2024-11-05

摘要/Abstract

摘要： 测定了从红树林分离出来的耐盐内生细菌阿耶波多氏芽孢杆菌（Bacillus aryabhattai）对植物的促生特性,研究了盐胁迫下其定殖前后对绿豆[Vigna radiata （Linn.） Wilczek]生长的影响。结果表明,阿耶波多氏芽孢杆菌对NaCl的最高耐受浓度为110 g/L,最高耐受pH为10.0,产IAA能力为（154.474±1.526）μg/mL、ACC脱氨酶活性为（0.125 6±0.051 2） U/mg、溶有机磷和无机磷能力分别为1.89±0.03和2.27±0.01;基因组DNA里扩增出appA和phoD两个基因,这是该菌具溶磷能力的分子机理。阿耶波多氏芽孢杆菌在绿豆根、茎、叶3个部位的定殖率分别为68.27 %、63.48%、50.00%,定殖组的绿豆幼苗叶绿素含量和SOD活性高于对照组,说明阿耶波多氏芽孢杆菌的定殖有助于绿豆幼苗抗氧化能力的提高,从而提高绿豆的耐盐性。

关键词: 阿耶波多氏芽孢杆菌（Bacillus aryabhattai）, 植物促生菌, 绿豆[Vigna radiata （Linn.） Wilczek], 耐盐性

Abstract: The characteristics of promoting plant growth of salt-tolerant endophytic bacterium Bacillus aryabhattai isolated from mangrove were determined, and the effects of its colonization into mung bean [Vigna radiata （Linn.） Wilczek] under salt stress were studied. The results showed that the highest NaCl tolerant concentration of B. aryabhattai was 110 g/L, the highest pH tolerant value was 10.0, the IAA production capacity was （154.474±1.526） μg/mL, the ACC deaminase activity was （0.125 6±0.051 2） U/mg, and the capacities of dissolving organic phosphorus and inorganic phosphorus were 1.89±0.03 and 2.27±0.01, respectively. Two genes, appA and phoD, were amplified in the genomic DNA, which was the molecular mechanism of the phosphorus-solubilizing ability of the bacteria. The colonization rates of B. aryabhattai in roots, stems and leaves were 68.27%, 63.48% and 50.00%, respectively. The chlorophyll content and superoxide dismutase （SOD） activity of mung bean seedlings in the colonization group were higher than those in the control group, indicating that the colonization of B. aryabhattai contributed to the improvement of the antioxidant capacity of mung bean seedlings, thus effectively increasing the salt tolerance of mung bean.

Key words: Bacillus aryabhattai, plant growth-promoting bacteria, mung bean [Vigna radiata （Linn.） Wilczek], salt tolerance

中图分类号:

刘彩婷, 叶颖婷, 范铭丰, 陈晓逸, 刘希华. 阿耶波多氏芽孢杆菌对盐胁迫下绿豆的促生效果[J]. 湖北农业科学, 2024, 63(10): 8-13.

LIU Cai-ting, YE Ying-ting, FAN Ming-feng, CHEN Xiao-yi, LIU Xi-hua. Growth-promoting effect of Bacillus aryabhattai on mung bean under salt stress[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(10): 8-13.

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阿耶波多氏芽孢杆菌对盐胁迫下绿豆的促生效果

Growth-promoting effect of Bacillus aryabhattai on mung bean under salt stress

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