外源褪黑素对干旱胁迫下金花菜种子萌发和试管苗生长发育的影响

doi:10.14088/j.cnki.issn0439-8114.2025.09.024

摘要/Abstract

摘要： 为探究外源褪黑素对金花菜（Medicago polymorpha L.）抗旱性的影响,以上海金花菜种子为材料,利用植物组织培养技术,设置5个聚乙二醇-6000（PEG-6000）浓度进行种子萌发和试管苗生长试验,筛选出PEG-6000的阈值浓度;在PEG-6000阈值浓度模拟干旱胁迫下,设置6个褪黑素（MT）浓度,探究外源褪黑素对干旱胁迫下金花菜种子萌发和试管苗生长发育的影响。结果表明,PEG-6000模拟干旱胁迫可显著抑制金花菜种子萌发及其幼苗的生长发育。在5% PEG-6000模拟干旱胁迫下,0.01%~0.05% MT处理可以促进金花菜种子的萌发及其幼苗的生长发育。外施0.01%~0.05% MT可以显著增加金花菜幼苗的CAT活性、SOD活性、POD活性、可溶性糖含量、可溶性蛋白含量和脯氨酸含量,显著上调WRKY转录因子、蛋白TIFY 8、转录因子MYB13、NAC转录因子47、ABC转运A家族成员7、晚期胚胎发生丰富蛋白2和乙烯反应转录因子ERF110等基因的表达,显著降低MDA含量。因此,适宜浓度的MT处理能有效缓解干旱胁迫对金花菜种子萌发和幼苗造成的损伤,显著增强金花菜幼苗的抗旱性。

关键词: 金花菜（Medicago polymorpha L.）, 干旱胁迫, 褪黑素, 种子萌发, 生长发育

Abstract: To investigate the effect of exogenous melatonin on the drought resistance of Medicago polymorpha L., this experiment used Medicago polymorpha L. seeds from Shanghai as materials and utilized plant tissue culture technology to set five concentrations of polyethylene glycol-6000 (PEG-6000) for seed germination and plantlet growth experiments, and screened the threshold concentration of PEG-6000; under simulated drought stress at a threshold concentration of PEG-6000, six concentrations of melatonin (MT) were set to investigate the effects of exogenous melatonin on seed germination and plantlet growth and development of Medicago polymorpha L. under drought stress. The results showed that PEG-6000 simulation of drought stress significantly inhibited seed germination and plantlet growth and development of Medicago polymorpha L.. Under 5% PEG-6000 simulated drought stress, 0.01%-0.05% MT treatment could promote the seed germination and plantlet growth and development of Medicago polymorpha L.. External application of 0.01%-0.05% MT could significantly increase CAT activity, SOD activity, POD activity, soluble sugar content, soluble protein content, and proline content in Medicago polymorpha L. seedlings, also significantly upregulated the expression of WRKY transcription factor, protein TIFY 8, transcription factor MYB13, NAC transcription factor 47, ABC transporter A family member 7, late embryogenesis rich protein 2, and ethylene responsive transcription factor ERF110, while significantly reducing their MDA content. Therefore, MT treatment at an appropriate concentration could effectively alleviate the damage caused by drought stress to the seed germination and seedlings of Medicago polymorpha L., and significantly enhance the drought resistance of Medicago polymorpha L. seedlings.

Key words: Medicago polymorpha L., drought stress, melatonin, seed germination, plantlet growth and development

中图分类号:

S541.9

夏瑾华, 袁昕. 外源褪黑素对干旱胁迫下金花菜种子萌发和试管苗生长发育的影响[J]. 湖北农业科学, 2025, 64(9): 148-156.

XIA Jin-hua, YUAN Xin. Effect of exogenous melatonin on seed germination and plantlet growth and development of Medicago polymorpha L. under drought stress[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 148-156.

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