一株高产纤维素酶乳酸菌的分离与鉴定

doi:10.14088/j.cnki.issn0439-8114.2025.09.025

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (9): 157-161.doi: 10.14088/j.cnki.issn0439-8114.2025.09.025

一株高产纤维素酶乳酸菌的分离与鉴定

尹杨燕, 黎春玲², 俸祥仁³, 苏亚权³, 余注光⁴, 陈忠伟¹, 龙波⁴, 黄平升⁴, 李常挺¹, 彭昊¹, 李裕华⁴, 刘玉军⁴

1.广西壮族自治区兽医研究所,南宁 530001;
2.广西大学动物科学技术学院,南宁 530004;
3.广西农业职业技术大学,南宁 530007;
4.广西壮族自治区南宁良凤江国家森林公园,南宁 530031

收稿日期:2025-06-10 出版日期:2025-09-25 发布日期:2025-10-28
通讯作者: 李裕华（1987-）,男,广西昭平人,硕士,主要从事林业技术研究和生产管理及营造林管理工作,（电子信箱）260077458@qq.com;共同刘玉军（1969-）,男,广西全州人,研究方向为森林资源培育,（电子信箱）598623826@qq.com。
作者简介:尹杨燕（1998-）,女,贵州贵阳人,在读博士研究生,研究方向为动物疫病与病原微生物防控,（电子信箱）1985717389@qq.com。
基金资助:
广西农业职业技术大学科技项目（XKJ2335）; 广西壮族自治区林业局科技项目（2024GXZCLK37); 广西重点研发计划项目（AB23075145; AB21238003; AB21220005-4; AB23075145）; 广西科学研究计划项目（24-2; 24-12; 24-10; 24-14; XKJ2325; XKJ2335）; 国家现代农业产业技术体系广西创新团队建设项目（nycytxgxcxtd-2021-09）

Isolation and identification of a cellulase-high-producing lactic acid bacterium strain

YING Yang-yan^1,2, LI Chun-ling², FENG Xiang-ren³, SU Ya-quan³, YU Zhu-guang⁴, CHEN Zhong-wei¹, LONG Bo⁴, HUANG Ping-sheng⁴, LI Chang-ting¹, PENG Hao¹, LI Yu-hua⁴, LIU Yu-jun⁴

1. Guangxi Veterinary Research Institute, Nanning 530001, China;
2. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
3. Guangxi Vocational University of Agriculture, Nanning 530007, China;
4. Guangxi Liangfengjiang National Forest Park, Nanning 530031, China

Received:2025-06-10 Published:2025-09-25 Online:2025-10-28

摘要/Abstract

摘要： 旨在通过开发高产纤维素酶菌株来提高纤维素资源的利用率、降低微生物饲料添加剂生产成本,采集健康奶牛瘤胃内容物,经分离纯化培养获得11株菌株,通过生理生化试验、PCR扩增和16S rDNA 测序确定菌株种属,选用刚果红羧甲基纤维素钠琼脂培养基进行产纤维素酶细菌菌株的初筛,并采用硝基水杨酸（DNS）法对产酶菌株进行酶活力测定。结果表明,筛选得到一株高产纤维素酶乳酸菌,经鉴定为副干酪乳杆菌,在优化培养条件下,以0.2%羧甲基纤维素钠（CMC-Na）作为底物,在40 ℃、pH 5.0条件下,纤维素酶活力可达到0.57 U/mL。

关键词: 纤维素酶, 产酶细菌, 乳酸菌, 酶活力

Abstract: The objective of this study was to enhance the utilisation of cellulose resources and reduce the production cost of microbial feed additives through the development of high-yielding cellulase strains. A total of 11 strains were isolated and cultured from the rumen contents of healthy dairy cows. The strains were identified by a combination of physiological and biochemical tests, PCR amplification and 16S rDNA sequencing. Congo red sodium carboxymethyl cellulose agar medium was selected for the initial screening of cellulase-producing bacterial strains, and the enzyme viability of the enzyme-producing strains was determined by the DNS method.Results showed that a high cellulase-producing strain of Lactobacillus paracasei was identified. In the context of optimally cultivated conditions, with 0.2% carboxymethyl cellulose sodium (CMC-Na) serving as the substrate, the cellulase activity attained 0.57 U/mL at 40 ℃ and pH 5.0.

Key words: cellulase, enzyme-producing bacteria, lactic acid bacterium, enzyme activity

中图分类号:

Q939.11⁺7

尹杨燕, 黎春玲, 俸祥仁, 苏亚权, 余注光, 陈忠伟, 龙波, 黄平升, 李常挺, 彭昊, 李裕华, 刘玉军. 一株高产纤维素酶乳酸菌的分离与鉴定[J]. 湖北农业科学, 2025, 64(9): 157-161.

YING Yang-yan, LI Chun-ling, FENG Xiang-ren, SU Ya-quan, YU Zhu-guang, CHEN Zhong-wei, LONG Bo, HUANG Ping-sheng, LI Chang-ting, PENG Hao, LI Yu-hua, LIU Yu-jun. Isolation and identification of a cellulase-high-producing lactic acid bacterium strain[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 157-161.

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一株高产纤维素酶乳酸菌的分离与鉴定

Isolation and identification of a cellulase-high-producing lactic acid bacterium strain

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