基于转录组的睡莲EST-SSR遗传多样性分析

doi:10.14088/j.cnki.issn0439-8114.2026.05.021

摘要/Abstract

摘要： 基于转录组开发睡莲(Nymphaea L.)EST-SSR标记,并进行遗传多样性分析,为睡莲种质资源鉴定、遗传多样性评估及分子标记辅助育种提供可靠工具。试验基于泰王睡莲(Nymphaea ‘King of Siam')花器官转录组数据拼接获得362 982条Unigene,从中开发出29对多态性EST-SSR引物,并结合9条ISSR引物对77份睡莲种质进行遗传多样性分析。结果表明,EST-SSR标记表现出较高的多态性,平均等位基因数(Na)为4.793 1,平均期望杂合度(He)为0.648 4。聚类分析表明,77份睡莲种质可划分为耐寒与热带两大类群,揭示出二者之间存在显著的遗传分化。ISSR标记分析结果与EST-SSR基本一致,但EST-SSR在多态性检测效率和分辨率方面更具优势。BLAST比对结果显示,20个SSR位点与开花调控、抗逆反应及重金属富集等相关功能基因显著关联。77份睡莲种质表现出丰富的遗传多样性,EST-SSR及ISSR可有效鉴定其耐寒与热带类群间的遗传分化,并表明EST-SSR标记的技术优势,可用于睡莲遗传多样性研究。

关键词: 睡莲(Nymphaea L.), EST-SSR标记, ISSR标记, 遗传多样性分析, 功能基因

Abstract: The development of expressed sequence tag-simple sequence repeat (EST-SSR) markers based on transcriptome data and subsequent genetic diversity analysis provided reliable tools for germplasm identification, genetic diversity evaluation, and marker-assisted breeding in water lily (Nymphaea L.). In this study, 362 982 unigenes were assembled from the floral organ transcriptome data of Nymphaea ‘King of Siam'. From these, 29 pairs of polymorphic EST-SSR primers were developed, and 9 inter-simple sequence repeat (ISSR) primers were utilized to analyze the genetic diversity of 77 water lily accessions. The results indicated that the EST-SSR markers exhibited a high level of polymorphism, with an average number of alleles (Na) of 4.793 1 and an average expected heterozygosity (He) of 0.648 4. Cluster analysis demonstrated that the 77 water lily accessions could be clearly classified into two major groups: hardy and tropical, revealing significant genetic differentiation between them. The analytical results derived from the ISSR markers were highly consistent with those from the EST-SSRs; however, the EST-SSR markers demonstrated superior advantages in terms of polymorphism detection efficiency and resolution. Furthermore, BLAST alignment revealed that 20 SSR loci were significantly associated with functional genes involved in flowering regulation, stress response, and heavy metal accumulation. In conclusion, the 77 water lily accessions exhibited rich genetic diversity. Both EST-SSR and ISSR markers effectively identified the genetic differentiation between the hardy and tropical groups, highlighting the technical superiority of EST-SSR markers as a robust tool for genetic diversity research in water lilies.

Key words: water lily(Nymphaea L.), EST-SSR marker, ISSR marker, genetic diversity, functional gene

中图分类号:

胡一航, 单松望, 陈龙清, 谯正林. 基于转录组的睡莲EST-SSR遗传多样性分析[J]. 湖北农业科学, 2026, 65(5): 130-141.

HU Yi-hang, SHAN Song-wang, CHEN Long-qing, QIAO Zheng-lin. Genetic diversity analysis of water lily based on transcriptome-derived EST-SSR markers[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(5): 130-141.

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