湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 157-159.doi: 10.14088/j.cnki.issn0439-8114.2019.23.039

• 贮藏·加工 • 上一篇    下一篇

大蒜提取物对荧光假单胞菌生物膜形成的抑制作用

綦国红, 巴云英, 杨志萍, 陈贵堂, 王海翔   

  1. 中国药科大学工学院,南京 211198
  • 收稿日期:2018-11-06 出版日期:2019-12-10 发布日期:2019-12-18
  • 作者简介:綦国红(1972-),男,内蒙古赤峰人,副教授,博士,主要从事食品微生物与生物技术研究,(电话)15951856501(电子信箱)qghbox@163.com。
  • 基金资助:
    细菌群体感应对鱼虾腐败过程的调控作用及其分子机制(30700627)

Inhibitory effect of garlic extract on the biofilm formation of Pseudomonas fluorescens

QI Guo-hong, BA Yun-ying, YANG Zhi-ping, CHEN Gui-tang, WANG Hai-xiang   

  1. School of Engineering,China Pharmaceutical University,Nanjing 211198,China
  • Received:2018-11-06 Online:2019-12-10 Published:2019-12-18

摘要: 研究大蒜提取物对荧光假单胞菌(Pseudomonas fluorescens)生物膜形成的抑制作用。用结晶紫染色法以及光学显微镜对该菌产生的生物膜进行了研究,苯酚硫酸法测定其胞外多糖的产量,采用多孔板法研究了大蒜提取物与山梨酸钾的协同作用。结果表明,大蒜提取物浓度为100、150 mg/mL时,对生物膜的抑制率分别为27.6%和38.7%;对胞外多糖抑制率分别为4.5%和12.6%;100 mg/mL的提取物与山梨酸钾具有协同作用。因此,大蒜提取物对荧光假单胞菌生物膜的形成具有抑制作用。

关键词: 荧光假单胞菌(Pseudomonas fluorescens), 生物膜, 大蒜提取物

Abstract: The inhibitory effect of garlic extract on biofilm development in Pseudomonas fluorescens was studied. The biofilm produced by the bacteria was determined using crystal violet staining and light microscope, phenol sulfuric acid method was used to test the production of extracellular polysaccharides, the synergistic effect of garlic extract with potassium sorbate was studied by porous plate method. The results showed that the inhibitory rates of garlic extract on biofilm development were 27.6% and 38.7%, the inhibitory rates on extracellular polysaccharide were 4.5% and 12.6%, respectively, at the concentration of the garlic extract 100 mg/mL and 150 mg/mL, garlic extract showed synergistic effect with potassium sorbate at the concentration of 100 mg/mL. Therefore, garlic extract inhibited the biofilm formation in P. fluorescens.

Key words: Pseudomonas fluorescens, biofilm, garlic extract

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