菊迪基氏菌生防菌解淀粉芽孢杆菌D2WM抑菌物质防治效果评价及药剂筛选

doi:10.14088/j.cnki.issn0439-8114.2020.02.018

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (2): 87-92.doi: 10.14088/j.cnki.issn0439-8114.2020.02.018

菊迪基氏菌生防菌解淀粉芽孢杆菌D2WM抑菌物质防治效果评价及药剂筛选

陈嘉敏^1,2, 刘惠英^1,2, 张向辉¹, 邓婷婷¹, 魏蜜¹

1. 湖北工程学院特色果蔬质量安全控制湖北省重点实验室/生命科学技术学院,湖北孝感 432000;
2. 湖北大学生命科学学院,武汉 430062

收稿日期:2019-03-28 发布日期:2020-04-24
通讯作者: 魏蜜（1987-）,女,湖北孝感人,副教授,博士,主要从事微生物学和植物病害生物防治,（电子信箱）weimi555@163.com。
作者简介:陈嘉敏（1995-）,女,湖北孝感人,在读硕士研究生，研究方向为作物学,（电话）13733427825（电子信箱）1002359952@qq.com
基金资助:
国家自然科学基金资助项目（31600055）；中国博士后项目（2018M642815）

Evaluation of the control effect of biocontrol bacteria Bacillus amyloliquefaciens D2WM on Dickeya chrysanthemi and its fungicides screening

CHEN Jia-min^1,2, LIU Hui-ying^1,2, ZHANG Xiang-hui¹, DENG Ting-ting¹, WEI Mi¹

1. Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables/College of Life Science and Technology,Hubei Engineering University,Xiaogan 432000,Hubei,China;
2. College of Life Science,Hubei University,Wuhan 430062,China

Received:2019-03-28 Published:2020-04-24

摘要/Abstract

摘要： 采用单因素试验方法优化生防菌解淀粉芽孢杆菌（Bacillus amyloliquefaciens）D2WM抑菌物质的提取效率,并对抑菌物质粗提取的储藏特性展开研究,同时利用市售药剂对菊迪基氏菌（Dickeya chrysanthemi）进行室内药剂筛选,对比生防菌剂与市售药剂的防治效果。结果表明,通过单因素优化提取生防菌解淀粉芽孢杆菌D2WM中的抑菌物质,发现最佳提取溶剂为正丁醇,有机溶剂与发酵液体积比为1∶7、提取时间为0.5 h;抑菌物质具有良好的热稳定性和紫外光照稳定性,在处理温度达100 ℃,紫外光照射12 h时,抑菌效果均没有显著性下降,抑菌圈仍有23 mm;当抑菌物质储存180 d后,抑菌圈大小仍有16.33 mm。生防菌解淀粉芽孢杆菌D2WM粗提物对黑腐果胶杆菌、胡萝卜软腐果胶杆菌、菊迪基氏菌、丁香假单胞菌和黄单胞菌等植物病原菌均有良好的抑菌效果。测定的19种药剂中对菊迪基氏菌有良好抑制作用的仅有新田秀才6号、中生菌素、噻霉酮和氯溴异氰尿酸4种;其中,以生防菌解淀粉芽孢杆菌D2WM粗提物作为对照药剂,发现其抑菌效果高于噻霉酮和氯溴异氰尿酸。

关键词: 解淀粉芽孢杆菌（Bacillus amyloliquefaciens）D2WM, 抑菌物质, 抑菌效果, 药剂筛选

Abstract: Single factor test was used to optimize the extraction efficiency of Bacillus amyloliquefaciens D2WM antimicrobial substance, and the storage characteristics of crude extraction of antimicrobial substance were studied. The stability of crude extracts and the fungicides screening on Dickeya chrysanthemi were studied. The results of biocontrol agents and commercial agents were compared. The results indicated that the optimum extraction solvent was n-butyl alcohol, the volume ratio of organic solvent to fermentation broth was 1∶7, and the extracting time was 0.5 h. In addition, the antimicrobial substances showed excellent stability towards temperature and ultraviolet. The antimicrobial activity was not influenced when exposed at 100 ℃, or after treatment of ultraviolet irradiation for 12 hours, the size of the antimicrobial circle was still 23 mm. When the antimicrobial substances were stored for 180 days, the size of the antimicrobial circle was still 16.33 mm. The crude extracts of biocontrol bacteria Bacillus amyloliquefaciens D2WM had good bacteriostatic effects on plant pathogens such as Pectobacterium atrosepticum, Pectobacterium carotovorum, Dickeya chrysanthemi, Pseudomonas syringae and Xanthomonas arboricola. Only New-tian Xiucai No. 6, Zhongshengmycin, benziothiazolinone and chloroisobromine cyanuric acid had good inhibitory effects on Dickeya chrysanthemi among the 19 tested medicines. It was found that the bacteriostatic effect of crude extract of biocontrol bacteria Bacillus amyloliquefaciens D2WM was higher than benziothiazolinone and chloroisobromine cyanuric acid.

Key words: Bacillus amyloliquefaciens D2WM, antibacterial substances, antagonism effect, fungicides screening

中图分类号:

S476

陈嘉敏, 刘惠英, 张向辉, 邓婷婷, 魏蜜. 菊迪基氏菌生防菌解淀粉芽孢杆菌D2WM抑菌物质防治效果评价及药剂筛选[J]. 湖北农业科学, 2020, 59(2): 87-92.

CHEN Jia-min, LIU Hui-ying, ZHANG Xiang-hui, DENG Ting-ting, WEI Mi. Evaluation of the control effect of biocontrol bacteria Bacillus amyloliquefaciens D2WM on Dickeya chrysanthemi and its fungicides screening[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(2): 87-92.

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菊迪基氏菌生防菌解淀粉芽孢杆菌D2WM抑菌物质防治效果评价及药剂筛选

Evaluation of the control effect of biocontrol bacteria Bacillus amyloliquefaciens D2WM on Dickeya chrysanthemi and its fungicides screening

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