基于醇溶蛋白的木兰科植物鉴定技术研究

doi:10.14088/j.cnki.issn0439-8114.2020.02.020

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (2): 98-101.doi: 10.14088/j.cnki.issn0439-8114.2020.02.020

基于醇溶蛋白的木兰科植物鉴定技术研究

叶珊珊^a, 王楠^a, 高艳芳^b, 唐密^a, 刘梦龄^a, 肖强^a

a. 湖北民族大学,生物资源保护与利用湖北省重点实验室,湖北恩施 445000;
b. 湖北民族大学,林学园艺学院,湖北恩施 445000

收稿日期:2019-04-20 发布日期:2020-04-24
通讯作者: 肖强,男,教授,主要从事植物生物化学与分子生物学研究,（电子信箱）275975926@qq.com。
作者简介:叶珊珊（1997-）,女,广西桂林人,在读本科生,研究方向为植物生物化学与分子生物学,（电话）16677160687（电子信箱）1271271042@qq.com
基金资助:
国家自然科学基金项目（31260057; 31460203）; 湖北省大学生创新创业训练计划项目（201710517038; 201810517051）

Study on the identification of Magnoliaceae plants based on the alcohol-soluble protein

YE Shan-shan^a, WANG Nan^a, GAO Yan-fang^b, TANG Mi^a, LIU Meng-ling^a, XIAO Qiang^a

a. Hubei key Laboratory of Biological Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,Hubei,China;
b. School of Forestry and Horticulture,Hubei Minzu University,Enshi 445000,Hubei,China

Received:2019-04-20 Published:2020-04-24

摘要/Abstract

摘要： 木兰科（Magnoliaceae）植物分布范围广且属种多样,属种之间形态结构差异存在许多过渡类型,在属与种之间的鉴定上存在很多争议。目前,对于木兰科植物种子分类和鉴定研究中,通常以植物学形状特征作为鉴定方法。为了探索对木兰科植物种子进行鉴别的有效途径,采用酸性聚丙烯酰胺凝胶电泳对10种木兰科植物种子醇溶蛋白进行分析。结果发现,10种木兰科植物共分离出61条谱带,条带数大多在6~7,最多为9条,最少的6条。利用Quantityone软件对电泳谱带进行分析,发现不同种之间谱带都存在差异性,可以通过种子醇溶蛋白谱带进行木兰科植物物种鉴别。

关键词: 木兰科（Magnoliaceae）, 醇溶蛋白, 聚丙烯酰胺凝胶电泳

Abstract: The Magnoliaceae plants are widely distributed and varied in genus and species. There are many controversies in the identification of genus and species because many transitional types were found among different genus and species. At present, botanical shape characteristics are usually used as identification methods for the classification and identification of magnoliaceae plants. In order to further identify the magnolia plants, the acid polyacrylamide gel electrophoresis was used to analyze the gliadin of magnolia plants. The results showed that 61 bands were separated from 10 species of magnolia,and most of the bands were between 6 and 7, with a maximum of 9, and the lowest 6. Using Quantityone software to analyze electrophoresis bands, it is found that there are differences between different bands, and species identification can be carried out through spectral bands.

Key words: Magnoliaceae, gliadin, polyacrylamide gel electrophoresis

中图分类号:

叶珊珊, 王楠, 高艳芳, 唐密, 刘梦龄, 肖强. 基于醇溶蛋白的木兰科植物鉴定技术研究[J]. 湖北农业科学, 2020, 59(2): 98-101.

YE Shan-shan, WANG Nan, GAO Yan-fang, TANG Mi, LIU Meng-ling, XIAO Qiang. Study on the identification of Magnoliaceae plants based on the alcohol-soluble protein[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(2): 98-101.

参考文献

[1] 夏国华,黄坚钦,汤庚国,等.木兰科常用绿化树种种子形态鉴别[J].南京林业大学学报(自然科学版),2005,29(5):97-100.
[2] 张冰,黄云晖,苏应娟,等.木兰科种子内种皮合点区形态观察[J].生态科学,1996(1):34-38.
[3] 宋明杰. 木兰科植物的繁殖技术[J].农业网络信息,2011(7):125-127.
[4] 马小英,焦根林.木兰科植物种子繁殖研究概况[J].种子,2009,28(10):54-57,63.
[5] 徐凤霞,吴七根.木兰科种子内种皮合点区形态及其系统学意义[J].植物分类学报,2002,40(3):260-270.
[6] YANG Y B,GUAN Y A,QIN L,et al.Studies on extraction contrition and acid polyacrylamide gel electrophoresis method (A-PAGE) of prolamin in foxtail millet seed[J].Seed,2010, 29(1):8-11.
[7] NAŁECZ D,SZERSZUNOWICZ I,DZIUBA M,et al. 2-DE Separation and identification of oat (Avena sativa L.) proteins and their prolamin fractions[J].Methods Mol Biol,2017,1536:235-251.
[8] NAŁECZ D,DZIUBA J,MINKIEWICZ P,et al. Identification of oat (Avena sativa) and buckwheat (Fagopyrum esculentum) proteins and their prolamin fractions using two-dimensional polyacrylamide gel electrophoresis[J].European food research & technology,2009,230(1):71-78.
[9] MONTEIRO P V,VIRUPAKSHA T K,RAO D R.Proteins of Italian millet:Amino acid composition,solubility fraction and electrophoresis of protein fractions[J].Journal of the science of food & agriculture,2010,33(11):1072-1079.
[10] MONTEIRO P V,SUDHARSHANA L,RAMACHANDRA G.Japanese barnyard millet(Echinochloa frumentacea):Protein content,quality and SDS-PAGE of protein fractions[J].Journal of the science of food & agriculture,2010,43(1):17-25.
[11] 李毳,柴宝峰,梁爱华,等.油松种子醇溶蛋白提取剂比较[J].应用与环境生物学报,2006,12(1):125-127.
[12] 王伟,印丽萍,刘许佳,等.种子醇溶蛋白提取及检测条件探索[J].西北植物学报,2007,27(1):21-27.
[13] BUSHUK W,ZILLMAN R.Wheat cultivar identification by gliadin electrophoregrams. I. apparatus,method and nomenclature[J].Can J Plant Sci,1978,58(2):505-515.
[14] 孙克江,张甜,曲梅丽,等.聚丙烯酰胺凝胶电泳法快速检测植物源性食品中的蛋白成分[J].食品安全质量检测学报,2017, 8(3):895-899.
[15] 颜廷进,李群,戴双,等.小麦醇溶蛋白和麦谷蛋白在品种鉴定中的应用研究[J].山东农业科学,2015,47(3):105-107,117.
[16] KIM W T,OKITA T W.Nucleotide and primary sequence of a major rice prolamine.[J].FEBS letters,1988,231(2):308-310.
[17] 颜启传,黄亚军,徐湲.我国适用的小麦和大麦种子醇溶蛋白聚丙烯酰胺凝胶电泳鉴定品种的标准程序[J].种子,1989(6):55-57.
[18] 李剑,靳晓东,杨秋生,等.7种木兰科常绿植物亲缘关系的ISSR和AFLP分析[J].河南农业大学学报,2013,47(3):289-295.
[19] 薛晓明,南程慧,杨荣治,等.木兰科7种植物花粉的扫描电镜观察[J].安徽农业大学学报,2017,44(6):1098-1103.
[20] 周志娟,梅承芳,邓登.大麦中的醇溶蛋白质[J].啤酒科技,2005(4):62-63.
[21] 邵锦震,邱昌恩,丁毅.不同提取剂对麦醇溶蛋白提取效果的电泳比较[J].植物科学学报,2003,21(3):262-266.
[22] 王伟,印丽萍,陈沁.高粱种子醇溶蛋白的提取条件[J].上海大学学报(自然科学版),2007,13(6):746-750.

基于醇溶蛋白的木兰科植物鉴定技术研究

Study on the identification of Magnoliaceae plants based on the alcohol-soluble protein

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