关键词: 潮间带, 沉积物, 细菌多样性, 连云港市

Abstract: Based on the 16S rRNA gene, high-throughput sequencing technology was used to analyze bacterial diversity and community structure in coastal intertidal zone sediments of Lianyungang City, Jiangsu Province. Three sampling points were set up in three typical coastal intertidal zones of Lianyungang City, and nine sediment samples were collected. Bacteria were enriched and cultured, and total DNA was extracted. IIIumina Miseq sequencing was conducted and sequencing results were analyzed. The results showed that the bacteria in the three sampling points were mainly composed of Proteus, Bacteroides, Firmicutes, Actinomycetes, Acidobacteria, Fusobacteria, Verruca, Bacillus, Planctomycetes, and Chloroflexi. Among them, Proteus was the dominant bacteria group, accounting for 99.4% of the total bacteria group. The diversity and richness of bacterial communities were as follows： Xugou sampling point>Xishu sampling point>Dongxilian Island sampling point.The principal component analysis of bacterial community structure and the sample cluster analysis of UniFrac distance were consistent with the environmental and ecological differences of the sample collection points. The different types of sediment and physical and chemical factors in the intertidal zone affected the bacterial diversity and community composition.

Key words: intertidal zone, sediments, bacterial diversity, Lianyungang City