湖北农业科学 ›› 2023, Vol. 62 ›› Issue (8): 95-101.doi: 10.14088/j.cnki.issn0439-8114.2023.08.015

• 园艺·特产 • 上一篇    下一篇

褪黑素预处理对草莓苗耐旱性的影响

缑一杰, 杨振华   

  1. 杨凌职业技术学院草莓工程技术研究中心,陕西 杨凌 712100
  • 收稿日期:2023-02-13 出版日期:2023-08-25 发布日期:2023-09-22
  • 通讯作者: 杨振华,(电话)18092186348(电子信箱)kushi523@163.com。
  • 作者简介:缑一杰(1995-),女,陕西延安人,助教,硕士,主要从事园艺学教学和草莓育种研究工作,(电话)18829355017(电子信箱)gouyijie1025@163.com
  • 基金资助:
    陕西省重点研发项目(2023-YBNY-043); 杨凌职业技术学院院内科学研究项目(ZK21-72); 陕西省科技厅一般项目“蔬菜温室大棚智慧化种植管控技术研发与应用”(2022NY-217)

Effects of melatonin pretreatment on drought resistance of strawberry seedlings

GOU Yi-jie, YANG Zhen-hua   

  1. Strawberry Engineering Technology Research Center, Yangling Vocational & Technical College, Yangling 712100, Shaanxi, China
  • Received:2023-02-13 Online:2023-08-25 Published:2023-09-22

摘要: 为研究外源褪黑素预处理对草莓苗耐旱性的影响,以草莓苗为试验材料,用不同浓度褪黑素对草莓苗“喷施+灌根”预培养后再进行干旱处理。结果表明,干旱严重抑制草莓生长,中低浓度(100~200 μmol/L)褪黑素预处理能够有效缓解干旱对草莓苗造成的生长胁迫,增强超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,抑制过氧化氢(H2O2)和丙二醛(MDA)的产生。同时,干旱胁迫使草莓苗逆境相关基因的表达量升高,外源褪黑素预处理能进一步提高其表达水平,并显著影响褪黑素合成途径关键酶基因的表达,以提高草莓苗的耐旱性。

关键词: 草莓苗, 褪黑素, 干旱胁迫, 抗氧化, 逆境基因表达

Abstract: In order to study the effects of melatonin pretreatment on drought resistance of strawberry seedlings, strawberry seedlings were treated with different concentrations of melatonin in “spray+root irrigation” pre-culture before drought treatment. The results showed that drought severely inhibited strawberry growth. Medium and low concentration(100~200 μmol/L) melatonin pretreatment could effectively alleviate the growth stress caused by drought on strawberry seedlings, enhance the activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT), and inhibit the production of hydrogen peroxide (H2O2) and malondialdehyde(MDA). Meanwhile, drought stress increased the expression levels of stress-related genes in strawberry seedlings, and exogenous melatonin pretreatment could further enhance the expression levels and significantly affect the expression of key enzyme genes of the melatonin synthesis pathway, so as to improve the drought resistance of the strawberry.

Key words: strawberry seedling, melatonin, drought stress, antioxidant, stress gene expression

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