玉米秸秆降解复合菌系的筛选及发酵产短链脂肪酸

doi:10.14088/j.cnki.issn0439-8114.2024.02.010

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (2): 55-58.doi: 10.14088/j.cnki.issn0439-8114.2024.02.010

玉米秸秆降解复合菌系的筛选及发酵产短链脂肪酸

汪文君, 刘国庆, 王志花

合肥工业大学食品与生物工程学院,合肥 230601

收稿日期:2022-11-22 出版日期:2024-02-25 发布日期:2024-03-14
通讯作者: 刘国庆（1963-）,男,教授,主要从事生物质资源高值化利用的研究,（电子信箱）13675512000@163.com。
作者简介:汪文君（1998-）,女,安徽黄山人,在读硕士研究生,研究方向为食品质量与安全,（电话）15256595484（电子信箱）980370781@qq.com。
基金资助:
安徽省省级重点基金项目（JZ2022QSKJ0010）

Screening of maize stover degradation complex strains and fermentation to produce short-chain fatty acids

WANG Wen-jun, LIU Guo-qing, WANG Zhi-hua

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601,China

Received:2022-11-22 Online:2024-02-25 Published:2024-03-14

摘要/Abstract

摘要： 利用筛选得到的复合菌系来发酵玉米秸秆,研究复合菌系对降解秸秆产短链脂肪酸的影响,并对微生物菌群结构组成进行解析。结果表明,复合菌系在前4 d的降解速度最快,秸秆、纤维素和木质素的降解率分别达30.35%、27.07%和14.71%,在12 d时,其降解率分别达40.02%、32.25%和20.93%。复合菌系的代谢产物以异丁酸和乙酸为主,其含量分别占总短链脂肪酸的36.46%和33.15%。由高通量测序可知,复合菌系中的优势菌属主要由Clostridium sensu stricto、Cellulosilyticum、Clostridium XIVa、Enterobacteriaceae和Peptostreptococcaceae等组成。

关键词: 玉米秸秆, 复合菌, 筛选, 降解, 短链脂肪酸

Abstract: The compound strains obtained by screening were used to ferment corn straw, the effects of the compound strains on degrading straw to produce short-chain fatty acids were studied, and the structural composition of the microbial consortium was analyzed. The results showed that the degradation rate of the compound strain was the fastest in the first 4 days. The degradation rates of straw, cellulose and lignin reached 30.35%, 27.07% and 14.71%, respectively. At 12 days, the degradation rates reached 40.02%, 32.25% and 20.93%, respectively. The metabolites of the compound strain were isobutyric acid and acetic acid, which accounted for 36.46% and 33.15% of the total short chain fatty acids, respectively. According to high-throughput sequencing, the dominant bacteria genera in the compound strains were mainly composed of Clostridium sensu stricto, Cellulosilyticum, Clostridium XIVa, Enterobacteriaceae and Peptostreptococcaceae.

Key words: corn straw, compound bacteria, screening, degradation, short chain fatty acids

中图分类号:

Q939.9

汪文君, 刘国庆, 王志花. 玉米秸秆降解复合菌系的筛选及发酵产短链脂肪酸[J]. 湖北农业科学, 2024, 63(2): 55-58.

WANG Wen-jun, LIU Guo-qing, WANG Zhi-hua. Screening of maize stover degradation complex strains and fermentation to produce short-chain fatty acids[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(2): 55-58.

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玉米秸秆降解复合菌系的筛选及发酵产短链脂肪酸

Screening of maize stover degradation complex strains and fermentation to produce short-chain fatty acids

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