舍饲条件下藏仔猪腹泻源大肠杆菌毒力和粘附因子基因检测与耐药性分析

doi:10.14088/j.cnki.issn0439-8114.2024.06.025

湖北农业科学 ›› 2024, Vol. 63 ›› Issue (6): 156-160.doi: 10.14088/j.cnki.issn0439-8114.2024.06.025

舍饲条件下藏仔猪腹泻源大肠杆菌毒力和粘附因子基因检测与耐药性分析

李龙¹, 谭占坤², 李文凤¹, 刘锁珠²

1.杨凌职业技术学院动物工程学院,陕西杨凌 712100;
2.西藏农牧学院动物科学学院,西藏林芝 860000

收稿日期:2022-10-16 出版日期:2024-06-25 发布日期:2024-06-26
通讯作者: 刘锁珠（1969-）,男,甘肃武威人,教授,硕士,主要从事藏猪养殖与繁育研究,（电话）13628949601（电子信箱）20733987@qq.com。
作者简介:李龙（1981-）,男,陕西杨凌人,教授,博士,主要从事动物营养与饲料科学研究,（电话）18991854089（电子信箱）lilong1101@126.com。
基金资助:
西藏自治区重点研发计划项目（XZ202001ZY0039N）; 国家自然科学基金项目（31760673）; 杨凌职业技术学院自然科学研究基金项目（A2019066）

Detection and drug resistance analysis of virulence and adhesion factor genes of Escherichia coli from Tibetan piglets with diarrhea under house feeding conditions

LI Long¹, TAN Zhan-kun², LI Wen-feng¹, LIU Suo-zhu²

1. College of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, Shaanxi, China;
2. College of Animal Science, Xizang Agricultural and Animal Husbandry University, Linzhi 860000, Tibet, China

Received:2022-10-16 Published:2024-06-25 Online:2024-06-26

摘要/Abstract

摘要： 为有效预防和治疗藏仔猪腹泻,采用PCR和Kirby-Bauer纸片扩散法对西藏林芝市舍饲藏仔猪分离的102株腹泻源大肠杆菌（Escherichia coli）毒力基因、粘附因子基因和耐药性进行检测。大肠杆菌毒力基因和菌毛粘附因子基因检测结果表明,检出率最高的菌毛粘附因子基因是F18（45.10%）,其次是F4（15.69%）,所有菌株均未检出F41基因;102株菌株中有37株菌株未检出菌毛粘附因子基因。毒力基因中检出率最高的是Stx2e基因（25.49%）,其次为STb：EAST-1基因（21.57%）和STa：STb基因（17.65%）。大肠杆菌菌毛粘附因子基因和非菌毛粘附因子基因检测结果表明,在检出AIDA-1基因的菌株中菌毛粘附因子基因（F18、F4）的菌株占比为44.73%,在检出paa基因的菌株中菌毛粘附因子基因（F18、F4）的菌株占比为54.17%;在检出eae基因的菌株中没有检测到F4基因。大肠杆菌对氯霉素、四环素、氨苄西林和链霉素表现出高的耐药性,对3种及以上抗生素产生耐药性的大肠杆菌菌株数占总菌株数的86.27%。

关键词: 藏仔猪, 腹泻源, 大肠杆菌（Escherichia coli）, 毒力基因, 耐药性, 菌毛粘附因子基因, 非菌毛粘附因子基因

Abstract: To effectively prevent and treat diarrhea in Tibetan piglets, PCR and Kirby Bauer paper diffusion methods were used,the virulence genes, adhesion factor genes and drug resistance of 102 strains of Escherichia coli from Tibetan piglets with diarrhea in Linzhi, Xizang were detected. The detection results of virulence genes and pili adhesion factor genes in Escherichia coli showed that the pili adhesion factor gene with the highest detection rate was F18 （45.10%）, followed by F4 （15.69%）, and no F41 gene was detected in all strains; among the 102 strains, the pili adhesion factor gene was not detected in 37 strains. The gene with the highest detection rate among virulence genes was Stx2e （25.49%）, followed by the STb：EAST-1 gene （21.57%） and the STa：STb gene （17.65%）. The detection results of Escherichia coli pili adhesion factor genes and non pili adhesion factor genes showed that 44.73% of the strains containing AIDA-1 gene had pili adhesion factor genes （F18, F4）, and 54.17% of the strains containing paa gene had pili adhesion factor genes （F18, F4）;no F4 gene was detected in the strains that the eae gene was detected. Escherichia coli exhibited high drug resistance to chloramphenicol, tetracycline, ampicillin, and streptomycin, with 86.27% of the total bacterial strains exhibiting resistance to three or more antibiotics.

Key words: Tibetan piglets, diarrhea source, Escherichia coli, virulence genes, drug resistance, pili adhesion factor genes, non pili adhesion factor genes

中图分类号:

S828

李龙, 谭占坤, 李文凤, 刘锁珠. 舍饲条件下藏仔猪腹泻源大肠杆菌毒力和粘附因子基因检测与耐药性分析[J]. 湖北农业科学, 2024, 63(6): 156-160.

LI Long, TAN Zhan-kun, LI Wen-feng, LIU Suo-zhu. Detection and drug resistance analysis of virulence and adhesion factor genes of Escherichia coli from Tibetan piglets with diarrhea under house feeding conditions[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(6): 156-160.

参考文献

[1] VIDAL A, AGUIRRE L, SEMINATI C, et al.Antimicrobial resistance profiles and characterization of Escherichia coli strains from cases of neonatal diarrhea in Spanish pig farms[J]. Veterinary sciences, 2020, 7(2):48.
[2] DO K H, BYUN J W, LEE W K.Virulence genes and antimicrobial resistance of pathogenic Escherichia coli isolated from diarrheic weaned piglets in Korea[J]. Journal of animal science and technology, 2020, 62(4):543-552.
[3] DO K H, BYUN J W, LEE W K.Prevalence of O-serogroups, virulence genes, and F18 antigenic variants in Escherichia coli isolated from weaned piglets with diarrhea in Korea during 2008—2016[J]. Journal of veterinary science, 2019, 20(1):43-50.
[4] 刘鑫宇,张辉,李坤,等.西藏林芝地区仔猪白痢血清型及毒力基因检测[J].中国兽医学报,2017,37(9):1670-1675.
[5] 王刚,索朗斯珠,强巴央宗.西藏藏猪源大肠杆菌毒力基因检测与分型[J].甘肃畜牧兽医,2017,47(6):75-78.
[6] BARANZONI G M, FRATAMICO P M, GANGIREDLA J, et al.Characterization shiga toxin subtypes and virulence genes in porcine shiga toxin-producing Escherichia coli[J]. Frontiers in microbiology, 2016(10):574.
[7] CHENG D, SUN H, XU J, et al.Prevalence of fimbial colonization factors F18ab and F18ac in Escherichia coli isolates from weaned piglets with edema and/or diarrhea in China[J]. Veterinary microbiology, 2005, 110(1-2):35-39.
[8] M100-S24,Performance standards for antimicrobial susceptibility testing; Twenty-fourth informational supplement[S].
[9] European committee on antimicrobial susceptibility testing. Breakpoint tables for 11 interpretation of MICs and zone diameters[S].
[10] MAGIORAKOS A P, SRINIVASAN A, CAREY R B, et al.Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance[J]. Clinical microbiology and infection, 2012, 18(3):268-281.
[11] DE LAGARDE M, VANIER G, DESMARAIS G, et al.A new multidrug-resistant enterotoxigenic Escherichia coli pulsed-field gel electrophoresis cluster associated with enrofloxacin non-susceptibility in diseased pigs[J]. Journal of applied microbiology, 2021, 130(3):707-721.
[12] 毕斓婷,冀亚路,袭恒豫,等.仔猪致泻性大肠杆菌毒力因子多重PCR检测方法的建立与应用[J].中国兽医学报, 2022, 42(2):1-6.
[13] 张敏,李旭廷,王斌,等.四川地区猪源致病性大肠杆菌的分离鉴定与耐药性检测[J].四川畜牧兽医,2018,45(10):28-29,32.
[14] NGELEKA M,PRITCHARD J,APPLEYARD G,et al.Isolation and association of Escherichia coli AIDA-I/STb, rather than EAST-1 pathotype, with diarrhea in piglets and antibiotic sensitivity of isolates[J]. Journal of veterinary diagnostic investigation, 2003, 15(3):242-252.
[15] NIEWERTH U, FREY A, VOSS T, et al.The AIDA autotransporter system is associated with F18 and stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea[J]. Clinical and diagnostic laboratory immunology, 2001, 8(1):143-149.
[16] ZHANG W, ZHAO M, RUESCH L, et al.Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US[J]. Veterinary microbiology, 2007, 123(1-3):145-152.
[17] LECLERC S, BOERLIN P, GYLESYLES C, et al.Paa, originally identified in attaching and effacing Escherichia coli, is also associated with enterotoxigenic E. coli[J]. Research in microbiology, 2007, 158(1):97-104.
[18] 杨海峰,陈晓兰,吴萌,等.仔猪腹泻源大肠杆菌耐药性调查及耐药基因检测[J].畜牧与兽医,2019,51(5): 66-69.
[19] 于吉锋,康润敏,谢晶,等.仔猪腹泻性大肠杆菌多黏菌素耐药基因mcr-1筛查及其多重耐药基因检测[J].黑龙江畜牧兽医,2020(11):86-91.

舍饲条件下藏仔猪腹泻源大肠杆菌毒力和粘附因子基因检测与耐药性分析

Detection and drug resistance analysis of virulence and adhesion factor genes of Escherichia coli from Tibetan piglets with diarrhea under house feeding conditions

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价

[1]	尹家银, 喻娇, 唐青海, 滕威, 阳坤, 曹馨, 徐晴, 刘博, 李泽. 一株仔猪产肠毒素大肠杆菌分离鉴定、耐药性分析及其卵黄抗体的制备[J]. 湖北农业科学, 2023, 62(4): 127-134.
[2]	王宁宁, 李晓月, 刘泽文, 刘威, 高婷, 周丹娜, 杨克礼, 郭锐, 梁婉, 陈文杰, 贝为成, 田永祥, 袁芳艳. 一株多重耐药产肠毒素大肠杆菌的分离鉴定及生物学特性的研究[J]. 湖北农业科学, 2021, 60(24): 160-163.
[3]	蔡松, 杨东成, 唐梅, 熊圆圆, 王金华, 王永泽. 一种快速脱盐制备大肠杆菌电转受态细胞的方法[J]. 湖北农业科学, 2020, 59(17): 170-174.
[4]	卢苑青, 张腾飞, 邵华斌, 曾驰. 鸡舍空气中葡萄球菌的分离鉴定及耐药性分析[J]. 湖北农业科学, 2020, 59(16): 118-121.
[5]	冯世文, 李军, 潘艳, 贺会利, 彭昊, 钟舒红, 胡帅, 杨威. 广西不同养殖模式猪源大肠杆菌耐药表型差异性分析[J]. 湖北农业科学, 2020, 59(1): 114-118.
[6]	王相伟. 工程大肠杆菌高效转化外源脂肪酸生成羟基脂肪酸的研究[J]. 湖北农业科学, 2020, 59(1): 152-155.
[7]	卢琴, 罗青平, 张腾飞, 罗玲, 汪宏才, 王红琳, 温国元, 张蓉蓉, 张文婷, 邵华斌. 生物膜、毒力基因和耐药基因对APEC的致病性作[J]. 湖北农业科学, 2019, 58(23): 132-135.
[8]	邵志勇, 陈夏冰, 杨文海, 何斌, 吴利军, 聂文豪, 金尔光, 陈洁, 李佳琳, 张翠丽. 武汉地区禽大肠杆菌的分离、鉴定与耐药模式探讨[J]. 湖北农业科学, 2019, 58(22): 141-144.
[9]	吴利军, 李佳琳, 陈夏冰, 万平民, 邵志勇, 何斌, 杨文海, 陈洁, 金尔光, 潘中宝, 童伟文, 周源, 刘武. 奶牛乳房炎主要病原菌的分离鉴定及耐药性研究[J]. 湖北农业科学, 2019, 58(21): 145-149.
[10]	吴利军, 周丹娜, 邵志勇, 金尔光, 姚艳丰, 王富国, 陈夏冰, 何斌, 杨文海, 刘武, 童伟文, 夏瑜, 陈洁. 仔猪腹泻大肠杆菌分离鉴定及血清型与耐药性分析[J]. 湖北农业科学, 2018, 57(6): 94-97.
[11]	向敏, 胡修忠, 程蕾, 余婕, 周源, 刘晓华, 卢顺, 高其双, 夏瑜, 陶弼菲, 王定发. 湖北省奶牛乳房炎的病原学分析[J]. 湖北农业科学, 2018, 57(12): 85-88.
[12]	邵莉萍, 李宏胜, 妥鑫, 张继瑜. 三种抗菌药物对羊源大肠杆菌抗菌活性的研究[J]. 湖北农业科学, 2018, 57(1): 86-88.