工程大肠杆菌高效转化外源脂肪酸生成羟基脂肪酸的研究

doi:10.14088/j.cnki.issn0439-8114.2020.01.035

湖北农业科学 ›› 2020, Vol. 59 ›› Issue (1): 152-155.doi: 10.14088/j.cnki.issn0439-8114.2020.01.035

工程大肠杆菌高效转化外源脂肪酸生成羟基脂肪酸的研究

王相伟

山东大学（威海）海洋学院,山东威海 264209

收稿日期:2019-01-04 出版日期:2020-01-10 发布日期:2020-01-10
作者简介:王相伟（1980-）,男,山东临沂人,实验师,博士,主要从事基因工程研究,（电话）15069448385（电子信箱）xiangweiwang2002@sdu.edu.cn
基金资助:
威海市科技发展计划项目（1070413421423）

Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli

WANG Xiang-wei

Marine College of Shandong University （Weihai）,Weihai 264209,Shandong,China

Received:2019-01-04 Online:2020-01-10 Published:2020-01-10

摘要/Abstract

摘要： 细胞色素P450单加氧酶（P450BM3）能特异性催化脂肪酸ω碳位羟基化反应生成羟基脂肪酸,在前期构建携带单加氧酶基因P450BM3工程大肠杆菌（Escherichia coli）转化脂肪酸的基础上,为解决外源脂肪酸在羟基化过程中的消耗和转运问题,构建了脂酰基辅酶A合成酶基因（fadD）缺失和外膜蛋白编码基因（fadL）过表达的工程菌株。摇瓶条件下羟基十二酸产量达到748.8 mg/L,转化率为93.6%。

关键词: 脂酰辅酶A合成酶, 外膜蛋白编码基因, 羟基脂肪酸, 重组大肠杆菌（Escherichia coli）

Abstract: Cytochrome P450BM3 can hydroxylates fatty acids into hydroxyfatty acids（HFAs） at the ω-positions with high entioselectivity. Based on the construction of Escherichia coli carrying P450BM3 gene to transform fatty acids. As an effort to solve inhibit fatty acid β-oxidation and increase outer membrane fatty acids transporter, the lipoacyl-coa synthase gene（fadD） deletion and outer membrane proteins encode gene（fadL） overexpression strains were built. Under shake-flask conditions, this recombinant strains produced 748.8 mg/L hydroxy dodecanoic acid, meanwhile the conversion rate was 93.6%.

Key words: lipoacyl-coa synthase gene（fadD）, outer membrane proteins encode gene（fadL）, hydroxy fatty acid, recombinant Escherichia coli

中图分类号:

TS206.4

王相伟. 工程大肠杆菌高效转化外源脂肪酸生成羟基脂肪酸的研究[J]. 湖北农业科学, 2020, 59(1): 152-155.

WANG Xiang-wei. Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 152-155.

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工程大肠杆菌高效转化外源脂肪酸生成羟基脂肪酸的研究

Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli

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