大豆花叶病毒武汉分离物全基因测序及系统进化分析

doi:10.14088/j.cnki.issn0439-8114.2025.09.036

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (9): 238-245.doi: 10.14088/j.cnki.issn0439-8114.2025.09.036

大豆花叶病毒武汉分离物全基因测序及系统进化分析

邓克林^1,3, 卢自华^1,3, 李艳娇¹, 杨远霄¹, 黎超¹, 陈水莲¹, 杨红丽¹, 单志慧^1,2, 郝青南^1,2, 陈海峰^1,2

1.中国农业科学院油料作物研究所,武汉 430062;
2.农业农村部油料作物生物学与遗传改良重点实验室,武汉 430062;
3.中国农业科学院研究生院,北京 100081

收稿日期:2025-01-23 出版日期:2025-09-25 发布日期:2025-10-28
通讯作者: 郝青南（1982-）,女（满族）,河北承德人,副研究员,博士,主要从事大豆遗传育种研究,（电话）18602711993（电子信箱）haoqingnan@caas.cn;单志慧（1964-）,女,河南郑州人,研究员,博士,主要从事大豆抗病研究,（电话）15392906693（电子信箱）shanzhihui@caas.cn。
作者简介:邓克林（1998-）,男,山东潍坊人,在读博士研究生,研究方向为大豆遗传育种,（电话）18369696664（电子信箱）18369696664@163.com;共同第一作者,卢自华（2001-）,女,辽宁大连人,在读硕士研究生,研究方向为大豆遗传育种,（电话）13604092003（电子信箱）13604092003@163.com。
基金资助:
国家重点研发计划项目（2022YFF1001504); 国家重大科技项目（2023ZD04037）

Complete genome sequencing and phylogenetic analysis of the Wuhan isolate of soybean mosaic virus

DENG Ke-lin^1,3, LU Zi-hua^1,3, LI Yan-jiao¹, YANG Yuan-xiao¹, LI Chao¹, CHEN Shui-lian¹, YANG Hong-li¹, SHAN Zhi-hui^1,2, HAO Qing-nan^1,2, CHEN Hai-feng^1,2

1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China;
2. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China;
3. Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2025-01-23 Published:2025-09-25 Online:2025-10-28

摘要/Abstract

摘要： 从武汉采集表现花叶症状的大豆叶片,通过RT-PCR检测和全基因组扩增,获得一株大豆花叶病毒（SMV）,命名为SMV-WH。SMV-WH的基因组全长为9 940 nt,其编码多聚蛋白的开放阅读框位于111~9 719 nt。序列分析表明,SMV-WH与GenBank中164个SMV的全基因组核苷酸序列一致性在74.88%~98.36%,氨基酸序列一致性为75.15%~99.09%,表明SMV-WH具有较高的遗传多样性。系统进化分析显示,SMV-WH与来自中国的分离株LJZ010、LJZ002、DSMZ PV-0841、DSMZ PV-0938、JSJJ00和SCSN及来自韩国的分离株Anseong聚为一簇,与Anseong和SCSN亲缘关系最近。蛋白1(P1)中E299K的突变改变了蛋白激酶C磷酸化位点,而辅助组分蛋白酶（HC-Pro）中G593S的突变改变了酪蛋白激酶Ⅱ磷酸化位点。三维结构预测表明这些突变引起了蛋白构象的变化。

关键词: 大豆花叶病毒, 武汉分离物, 全基因测序, 系统进化

Abstract: Soybean leaves showing mosaic symptoms were collected from Wuhan, and a soybean mosaic virus (SMV) strain, named SMV-WH, was obtained through RT-PCR detection and whole genome amplification. The complete genome of SMV-WH was 9 940 nt in length, with an open reading frame encoding a polyprotein located at 111~9 719 nt. Sequence analysis showed that the whole genome nucleotide sequence identity between SMV-WH and 164 SMV strains from GenBank ranged from 74.88% to 98.36%, and the amino acid sequence identity ranged from 75.15% to 99.09%, indicating that SMV-WH had high genetic diversity. Phylogenetic analysis showed that SMV-WH clustered with Chinese isolates LJZ010, LJZ002, DSMZ PV-0841, DSMZ PV-0938, JSJJ00 and SCSN, and Korean isolate Anseong, and was most closely related to Anseong and SCSN. The E299K mutation in the protein 1（P1） altered the protein kinase C phosphorylation site, while the G593S mutation in the helper component proteinase(HC-Pro) changed the casein kinase Ⅱ phosphorylation site. Three-dimensional structure prediction indicated that these mutations caused changes in protein conformation.

Key words: soybean mosaic virus, Wuhan isolate, whole genome sequencing, phylogenetic analysis

中图分类号:

S435.2

邓克林, 卢自华, 李艳娇, 杨远霄, 黎超, 陈水莲, 杨红丽, 单志慧, 郝青南, 陈海峰. 大豆花叶病毒武汉分离物全基因测序及系统进化分析[J]. 湖北农业科学, 2025, 64(9): 238-245.

DENG Ke-lin, LU Zi-hua, LI Yan-jiao, YANG Yuan-xiao, LI Chao, CHEN Shui-lian, YANG Hong-li, SHAN Zhi-hui, HAO Qing-nan, CHEN Hai-feng. Complete genome sequencing and phylogenetic analysis of the Wuhan isolate of soybean mosaic virus[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 238-245.

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大豆花叶病毒武汉分离物全基因测序及系统进化分析

Complete genome sequencing and phylogenetic analysis of the Wuhan isolate of soybean mosaic virus

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