异毛远盲蚓在Cd胁迫下五种酶类标志物的响应特点

doi:10.14088/j.cnki.issn0439-8114.2019.12.014

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (12): 58-63.doi: 10.14088/j.cnki.issn0439-8114.2019.12.014

异毛远盲蚓在Cd胁迫下五种酶类标志物的响应特点

武静慧¹, 李永², 魏源³, 杨晓进¹, 张一², 胡清²

1.北京化工大学化学工程学院,北京 100029;
2.南方科技大学环境科学与工程学院,广东深圳 518055;
3.中国环境科学研究院环境基准与风险评估国家重点实验室,北京 100012

收稿日期:2019-01-25 出版日期:2019-06-25 发布日期:2019-12-05
通讯作者: 张一,男,博士,（电子信箱）zhangy3@sustc.edu.cn;胡清,女,教授,（电子信箱）huq@sustc.edu.cn。
作者简介:武静慧（1993-）,女（回族）,山东济宁人,在读硕士研究生,研究方向为土壤基准,（电话）18810868597（电子信箱）573067334@qq.com。
基金资助:
环境基准与风险评估国家重点实验室自由探索项目（K17291501）

Response characteristics of five enzymatic markers in earthworm （Amynthas heterochaetus） under Cd stress

WU Jing-hui¹, LI Yong², WEI Yuan³, YANG Xiao-jin¹, ZHANG Yi², HU Qing²

1.College of Chemical Engineering,Beijing University of Chemical Technology,Beijing 100029,China;
2.School of Environmental Science & Engineering,Southern University of Science and Technology,Shenzhen 518055,Guangdong,China;
3.State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Science,Beijing 100012,China

Received:2019-01-25 Online:2019-06-25 Published:2019-12-05

摘要/Abstract

摘要： 分析了中国北方土壤广布种——异毛远盲蚓（Amynthas heterochaetus）在重金属污染物镉（Cd）胁迫下的半致死剂量（LC₅₀）及与其免疫相关的5种酶类标志物在此过程中的活性变化,并与OECD推荐的标准蚯蚓种——赤子爱胜蚓（Eisenia fetida）进行了比较研究。结果表明,Cd对异毛远盲蚓24和48 h的LC₅₀（分别为11.23和5.17 μg/cm²）均小于赤子爱胜蚓（分别为21.99和16.95 μg/cm²）;低浓度Cd胁迫下,异毛远盲蚓体内SOD、GSH-PX、CAT、AchE、T-AOC的活性均发生了显著变化;与赤子爱胜蚓相比,异毛远盲蚓体内的酶类标志物响应快速（3 d）且持续时间较长（28 d）。

关键词: 环境基准, 蚯蚓, 异毛远盲蚓（Amynthas heterochaetus）, Cd, 酶类标志物

Abstract: Amynthas heterochaetus, a widely distributed soil species in northern China, was analyzed under heavy metal cadmium （Cd） stress at a semi-lethal dose （LC₅₀）. The activities of Amynthas heterochaetus and its five immune-related enzyme markers under Cd stress were analyzed and compared with Eisenia fetida, a standard earthworm species recommended by OECD. The results showed that LC₅₀ of Amynthas heterochaetus（respectively 11.23 and 5.17 μg/cm²） of Cd at 24 and 48 h was lower than Eisenia fetida（respectively 21.99 and 16.95 μg/cm²）. Under the low concentration of Cd stress, the activities of SOD, GSH-PX, CAT, AchE and T-AOC were significantly shifted, both in Amynthas heterochaetus and Eisenia fetida. Compared to Eisenia fetida, the response of enzymatic biomarkers in Amynthas heterochaetus were quicker （3 days） and lasted longer （28 days）.

Key words: environmental benchmark, earthworm, Amynthas heterochaetus, Cd, enzyme markers

中图分类号:

X174

武静慧, 李永, 魏源, 杨晓进, 张一, 胡清. 异毛远盲蚓在Cd胁迫下五种酶类标志物的响应特点[J]. 湖北农业科学, 2019, 58(12): 58-63.

WU Jing-hui, LI Yong, WEI Yuan, YANG Xiao-jin, ZHANG Yi, HU Qing. Response characteristics of five enzymatic markers in earthworm （Amynthas heterochaetus） under Cd stress[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 58-63.

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异毛远盲蚓在Cd胁迫下五种酶类标志物的响应特点

Response characteristics of five enzymatic markers in earthworm （Amynthas heterochaetus） under Cd stress

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