黄曲霉拮抗菌B10-6-1脂肽类抗生素粗提物的性质与抑菌机理研究

doi:10.14088/j.cnki.issn0439-8114.2019.14.017

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (14): 70-75.doi: 10.14088/j.cnki.issn0439-8114.2019.14.017

黄曲霉拮抗菌B10-6-1脂肽类抗生素粗提物的性质与抑菌机理研究

张学雯¹, 王奕丁², 李红亚¹, 王树香¹, 王全¹, 李术娜¹

1.河北农业大学生命科学学院,河北保定 071001;
2.新疆师范大学地理科学与旅游学院,乌鲁木齐 830054

收稿日期:2019-01-05 出版日期:2019-07-25 发布日期:2019-12-06
通讯作者: 李术娜（1972-）,女,河北保定人,教授,主要从事农业微生物方面的研究,（电子信箱）bdlishuna@126.com。
作者简介:张学雯（1992-）,女,河北黄骅人,在读硕士研究生,研究方向为微生物与生化药学,（电话）15032280315（电子信箱）2891136322@qq.com。
基金资助:
河北省教育厅科学技术研究项目（2011232）

Studies on the properties and antibacterial mechanism of crude lipopeptide extracts from Aspergillus flavus antagonistic bacterium Bacillus Amyloliquefaciens B10-6-1

ZHANG Xue-wen¹, WANG Yi-ding², LI Hong-ya¹, WANG Shu-xiang¹, WANG Quan¹, LI Shu-na¹

1.College of Life Science,Hebei Agricultural University,Baoding 071001,Hebei,China;
2.College of Geography Science and Tourism,Xinjiang Normal University,Urumqi 830054,China

Received:2019-01-05 Online:2019-07-25 Published:2019-12-06

摘要/Abstract

摘要： 采用酸沉醇提法从菌株B10-6-1发酵液中提取脂肽类抗生素粗提物,采用抑菌圈法确定其最低抑菌浓度及抑菌谱,考察温度、pH、蛋白酶、金属离子、EDTA等因素对其抑菌活性的影响;通过孢子萌发法和显微观察法考察脂肽类抗生素粗提物对黄曲霉（Aspergillus flavus）菌孢子萌发和菌丝的作用。结果表明,脂肽类抗生素粗提物的最低抑菌浓度为4 g/L,在40 ℃,pH为8时抑菌活性最高;温度、pH、蛋白酶、金属离子、EDTA对脂肽类抗生素粗提物抑菌活性都没有较明显的影响;该粗提物抑菌谱广泛,对白菜黑斑菌、小麦纹枯菌等9株真菌都有明显抑制作用。脂肽类抗生素粗提物处理菌丝可使菌丝细胞壁出现消融,并使孢子的萌发率降低,即该物质是通过抑制黄曲霉菌孢子萌发和菌丝生长起到抑菌作用的。

关键词: 黄曲霉（Aspergillus flavus）拮抗菌, 脂肽类抗生素粗提物, 性质鉴定, 抑菌机理

Abstract: The crude lipopeptide extract was extracted from the fermentation broth of strain B10-6-1 by acid precipitation. The minimum inhibitory concentration and the antimicrobial spectrum were determined by the inhibition circle method. The effects of temperature, pH, protease, metal ions, EDTA and other factors on the bacteriostatic activity were investigated. The effects of crude lipopeptide extracts on Aspergillus flavus spore germination and hyphae were investigated by spore germination and microscopic observation. The results showed that the minimum inhibitory concentration of crude lipopeptide extract was 4 g/L, with the highest inhibitory activity at 40 ℃ and pH of 8, while temperature, pH, protease, metal ion and EDTA had no obvious effect on the antibacterial activity of crude lipopeptide extract. The antifungal spectrum of the crude extract was extensive and had obvious inhibitory effect on 9 strains of fungi such as Alternaria brassicae and Rhizoctonia cerealis etc. The mycelium treated with lipopeptide extract could dissolve the site of mycelium cell wall and decrease the germination rate of spores, which could inhibit the germination of Aspergillus flavus spores and the growth of hyphae.

Key words: Aspergillus flavus antagonistic bacteria, crude extract of lipopeptide antibiotics, characterization, antibacterial mechanism

中图分类号:

S476

张学雯, 王奕丁, 李红亚, 王树香, 王全, 李术娜. 黄曲霉拮抗菌B10-6-1脂肽类抗生素粗提物的性质与抑菌机理研究[J]. 湖北农业科学, 2019, 58(14): 70-75.

ZHANG Xue-wen, WANG Yi-ding, LI Hong-ya, WANG Shu-xiang, WANG Quan, LI Shu-na. Studies on the properties and antibacterial mechanism of crude lipopeptide extracts from Aspergillus flavus antagonistic bacterium Bacillus Amyloliquefaciens B10-6-1[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(14): 70-75.

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黄曲霉拮抗菌B10-6-1脂肽类抗生素粗提物的性质与抑菌机理研究

Studies on the properties and antibacterial mechanism of crude lipopeptide extracts from Aspergillus flavus antagonistic bacterium Bacillus Amyloliquefaciens B10-6-1

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