Effects of Cercospora arachidicola Infection on Active Oxygen Metabolism and Defense Enzymes Changes in Peanut Varieties with Different Resistance

doi:10.14088/j.cnki.issn0439-8114.2018.16.012

Abstract

Abstract: Three varieties of peanut varieties with different resistant peanut varieties were determined by the changes of O₂^-, MDA content and defense enzyme content after the stress of Cercospora arachidicola,and the correlation between them and the disease resistance was analyzed. The results showed that the peanut variety tested bacteria inoculated leaf spot within 1~15 d,O₂^- and MDA content were presented increased first and then decreased trend. Among them, for the content of O₂^-, Yueyou No.7 reached maximumafter 9 d,and Huayu No.20 reached maximum after 7 d, and Luhua No.11 reached maximum after 5 d;For the content of MDA,Yueyou No.7 peaked after 9 d, Huayu No.20 reached a maximum peak after 9 d, Luhua No.11 reached the maximum after 13 d. The comparative analysis of CAT,SOD,POD,PAL and PPO in the leaves after inoculation showed that Luhua No.11 was the highest, followed by the Huayu No.20, and the lowest in Yueyou No.7. There was a negative correlation between the disease index and the protective enzyme content of the three kinds of peanut varieties,and there was a significant difference between the SOD and the PAL content in disease-resistant cultivars and susceptible varieties. Indicated that the peanut leaf spot after bacteria infection, oxygen anion and outbreak time and MDA content in leaf change were closely related to the resistance of peanut varieties, the stronger the resistance of peanut varieties response time more quickly, the smaller the leaf cell damage,and cell function lost the less; Different peanut varieties for leaf spot resistance level differences, rhizoctonina bacteria stress could cause the peanut leaf defense enzymes and antioxidants content changes, and the stronger the resistance of peanut varieties defense enzyme content was higher; The content of SOD and PAL could be used as the physiological and biochemical indexes to identify the resistance of peanut to C. arachidicola.

Key words: Cercospora arachidicola Hori, different resistance, active oxygen metabolism, defense enzymes

CLC Number:

S435.652

LIN Ying, ZHANG Hai-dong, XIE Jin-hui, LIU Xin-yu, ZANG Chao-qun, YU Shu-yi, HUANG Yu-qian, LIANG Chun-hao. Effects of Cercospora arachidicola Infection on Active Oxygen Metabolism and Defense Enzymes Changes in Peanut Varieties with Different Resistance[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(16): 51-56.

References

[1] SHOKES F M,BERGER R D,SMITH D H,et al.Reliability of disease assessment procedures:A case study with late leafspot of peanut[J].Oleagineux,1987,42(6):245-251.
[2] 孙大容. 花生育种学[M].北京:中国农业出版社,1998.
[3] STARKEY T E.A simplified medium for growing Cercospora arachidicola[J].Phytopathology,1980,70(10):990-991.
[4] 董炜博,石延茂,赵志强,等.花生品种(系)叶部病害综合抗性鉴定[J].中国油料作物学报,2000,22(3):71-74.
[5] BAKER C J,ORLANDI E W.Active oxygen in plant pathogenesis[J].Annu Rev Phytopathol,1995,33:299-321.
[6] GLAZENER J A,ORLANDI E W,BAKER C J.The active oxygen response of cell suspensions to incompatible bacteria is not sufficient to cause hypersensitive cell death[J].Plant Physiol,1996,110:759-763.
[7] AKCAY C U,ERCAN O,KAVAS M,et al.Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L) seedlings[J].Plant Growth Regulation,2010,61(1):21-28.
[8] 姜慧芳,任小平.干旱胁迫对花生叶片SOD活性和蛋白质的影响[J].作物学报,2004,30(2):169-174.
[9] 郭红莲,陈捷,高增贵,等.玉米灰斑病抗性机制中活性氧代谢的作用[J].植物保护学报,2003,30(2):133-137.
[10] KALIR A,POLJAKOFF-MAYBER A.Changes in activity of malate dehydrogenase,catalase,peroxidase and superoxide dismutase in leaves of Halimione protulacoides(L.) Allen exposed to high sodium chloride concentrations[J].Ann Bot,1981,47(1):75-85.
[11] 吴岳轩,曾富华,王容臣.杂交水稻对白叶枯病的诱导抗病性与细胞内防御酶系统关系的初步研究[J].植物病理学报,1996, 26(2):127-131.
[12] 陈捷,蔺瑞明,高增贵,等.玉米弯孢叶斑病病菌毒素对寄主防御酶系活性的影响及诱导抗性效应[J].植物病理学报,2002, 32(1):43-48.
[13] 陈莲,陈梦茵,林河通,等.解偶联剂DNP处理对采后龙眼果实果皮褐变和活性氧代谢的影响[J].中国农业科学,2009,42(11):4019-4026.
[14] 赵世杰,许长成,邹琦,等.植物中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
[15] 薛应龙. 植物生理学实验手册[M].上海:上海科技出版社,1985.
[16] 汤章城,王国强,史益敏,等.现代植物生理学实验指南[M].北京:科学出版社,1999.
[17] PELLEGRINI L,ROHFRITSCH O,FRITITG B,et al.Phenylalanine ammonia-lyase in tobacco[J].Plant Physiology,1994, 106(3):877-886.
[18] MISHRA B B,GAUTAM S,SHARMA A.Purification and characterization of polyphenol oxidase(PPO) from eggplant (Solanum melongena)[J].Food Chem,2012,134(4):1855-1861.
[19] RIZHSKY L,LIANG H J,MITTLER R.The combined effect of drought stress and heat shock on gene expression in tobacco[J].Plant Physiology,2002,130(3):1143-1151.
[20] 杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-125.
[21] WANG X M.Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development,and stress responses[J].Plant Physiology,2005,139(2):566-573.
[22] 贺字典,高增贵,庄敬华,等.玉米丝黑穗病菌对寄主防御相关酶活性的影响[J].玉米科学,2006,14(2):150-151,155.
[23] 曹晶晶,于子超,张颖,等.外源褪黑素对苹果采后灰霉病的防效及防御酶活性的影响[J].植物生理学报,2017,53(9):1753-1760.
[24] 黄玉茜,韩晓日,杨劲峰,等.连作胁迫对花生叶片防御酶活性及丙二醛含量的影响[J].吉林农业大学学报,2013,35(6):638-645.
[25] 宋培玲,张键,郝丽芬,等.不同抗性油菜品种接种黑胫病菌防御酶活性变化研究[J].华北农学报,2015,30(2):110-115.
[26] 马莹莹,贾娇,苏前富,等.玉米抵御玉蜀黍尾孢菌侵入的生理机制[J].植物保护学报,2015,42(3):340-346.
[27] 周瑜,刘佳佳,张盼盼,等.糜子叶片防御酶系及抗氧化物质对黑穗病菌胁迫的响应[J].中国农业科学,2016,49(17):3298-3307.
[28] 袁广胜,赵茂俊,张志明,等.串珠镰刀菌引起玉米穗粒腐病防御酶变化及其电镜观察(英文)[J].植物病理学报,2011,41(4):385-392.