Abstract: The genetic diversity of Akebia trifoliata（Thunb.） Koidz. in six different areas of Hubei and Hunan provinces was studied by RAPD molecular marker. A total of 86 DNA fragments were screened out from 50 random primers with 10 clear bands,obvious polymorphism and good repeatability. Among them,73 were polymorphic bands,accounting for the total number of bands amplified of 84.9%. Cluster analysis using NTSYS software,the similarity coefficient of 0.578 as a standard,all varieties can be divided into three categories,the major category Ⅰ includes samples from Enshi Hongmiao（purple type） and Hunan Guzhang county（brown type）; the category Ⅱ includes samples from Jianshi Huaping,Badong Chadianzi and Xuan’en Jiaoyuan;Samples from Enshi Taiyanghe is divided into a independent category.

Key words: Akebia trifoliata（Thunb.） Koidz., wild resources, RAPD, genetic relationship