Spectroscopic Studies of Interaction between Matrine and HSA and BSA

doi:10.14088/j.cnki.issn0439-8114.2018.11.024

Abstract

Abstract: The interaction between matrine （MAT） and human serum albumin （HSA） and bovine serum albumin （BSA） were investigated by fluorescence spectroscopy,UV absorption spectroscopy and circular dichroism spectroscopy with the simulated physiological condition. The experiment results of show UV absorption spectroscopy and fluorescence spectroscopy that matrine can react with HSA or BSA and form a kind of new compound at the ground state and prove that the flurescence quenching of HSA or BSA by matrine is dynamic quenching,and at different temperature,quenching constants are respectively HSA：4.60×10³,1.71×10⁴,3.07×10⁴ L/mol and BSA：3.17×10⁴、3.91×10⁴ and 4.48×10⁴ L/mol; binding constants are respectively HSA：5.999×10⁵、3.097×10⁶,6.667×10⁷ L/mol and 1.007×10⁵,1.163×10⁵,1.582×10⁵ L/mol, the number of binding sites is approximately equal to 1. The calculation results of thermodynamic parameters display that the reaction is hydrophobic effect and is spontaneous reaction between matrine and HSA or BSA.

Key words: matrine, HSA, BSA, fluorescence spectroscopy, UV absorption spectroscopy, circular dichroism spectroscopy

CLC Number:

YANG Mei-ling, CUI Dong-ya, WANG Li-xue, ZHANG Jie. Spectroscopic Studies of Interaction between Matrine and HSA and BSA[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(11): 96-99.

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