Effects of CdCl2 Treatment on the Growth and Protein Expression Level of Nicotiana benthamiana Seedlings

doi:10.14088/j.cnki.issn0439-8114.2018.21.021

Abstract

Abstract: In order to investigate the effects of CdCl₂ treatment on the growth and protein expression of Nicotiana benthamiana,the seedlings were treated with CdCl₂. The growth status of the seedlings was observed and the length of the root and stem was recorded. The protein of Nicotiana benthamiana were extracted by SDS extraction,and isolated by SDS-PAGE. A large number of experimental data showed that low concentration of CdCl₂ could promote the growth of the seedlings,while the high concentration of CdCl₂ inhibited the growth of the seedlings. When the concentration of CdCl₂ reached 0.5 mmol/L,the growth of seedlings began to be inhibited. If the concentration of CdCl₂ was greater than 1.5 mmol/L,the seedlings turned yellow and withered. SDS-PAGE showed that low concentration of CdCl₂ could increase the total protein content of the plant,but with the increase of CdCl₂ concentration,the total protein content of Nicotiana benthamiana significantly decreased.

Key words: CdCl₂, Nicotiana benthamiana, seedling, total protein, SDS-PAGE

CLC Number:

S572

LU Yu-jian, SHI Dong-li, WU Tao. Effects of CdCl₂ Treatment on the Growth and Protein Expression Level of Nicotiana benthamiana Seedlings[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(21): 87-90.

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Effects of CdCl₂ Treatment on the Growth and Protein Expression Level of Nicotiana benthamiana Seedlings

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