Abstract: Cardamine hupingshanensis seeds was cultivated in MS culture medium with different sodium selenate concentration （0, 20, 40, 60, 80, 100, 200, 300 mg/L）. The physiological and biochemical indexes such as seed germination rate, seedling survival rate, yellowing rate, plant height, root length, fresh weight, dry weight, POD activity and CAT activity were measured to discuss the response of selenate stress in pots to Cardamine hupingshanensis. The results showed that, the low concentration of sodium selenate was used to promote the growth and biomass of the Cardamine hupingshanensis, on the contrary, it would inhibit it. When the concentration of sodium selenate was 40 mg/L, Cardamine hupingshanensis had the highest germination rate and seedling rate, at the same time, the root length, plant height, fresh weight and dry weight of the seedlings reached the maximum value. The yellowing rate of Cardamine hupingshanensis seedlings increased with the increase of selenium salt concentration. The POD activity of the Cardamine hupingshanensis was decreased first and then increased, the lowest value reached at 40mg/L sodium selenate solution. The CAT activity of the Cardamine hupingshanensis was increased first and then decreased, the highest value reached at 100 mg/L sodium selenate solution.

Key words: Cardamine hupingshanensis, Se stress, physiological property