Abstract: Organochlorine pesticides in the roots of Tripterygium wilfordii were extracted by ultrasonic-reflux method,purified by sulfonation-silica gel column, and determined by gas chromatography. The results showed that this batch comes from six new species of Tripterygium wilfordii （TJ,TG,TZ,YJ,YG,YZ） planted in Yangxin, Hubei province. The pesticide DDT residues in their roots are 0.004 5,0.001 4,0.000 8,0.001 2,0.003 6,0.003 3 μg/g respectively; the pesticide BHC residues in their roots are 0.027 6,0.061 4,0.056 3,0.043 2,0.048 7,0.032 7 μg/g respectively; the pesticide PCNB residues in their roots are 0.001 9,0.001 8,0.001 5,0.001 6,0.001 6,0.001 9 μg/g respectively. All of which are less than the organochlorine residue standard for Chinese herbal medicine stipulated in the first part of Chinese pharmacopoeia 2015 edition. In this study, the pesticide content of Tripterygium wilfordii was determined by gas chromatography, and the results provides a scientific basis for the quality assurance of Tripterygium wilfordii. In terms of pesticide residue, the new planting base in Yangxin, Hubei province was suitable for planting of Tripterygium wilfordii.

Key words: Tripterygium wilfordii, BHC, DDT, PCNB, GC