The study of antimicrobial drugs screening using Connectivity Map database

doi:10.14088/j.cnki.issn0439-8114.2021.24.024

Abstract

Abstract: In order to screen new antibacterial agents and verify their antibacterial activity in vitro, the antimicrobial agents were screened by Connectivity Map （Cmap） database based on the gene chip results of streptococcus suis infected THP-1 cells. Antibacterial potential drugs were screened and the minimum inhibitory concentration（MIC） and the inhibitory effect on hemolytic activity of Escherichia coli, Pseudomonas aeruginosa, Staphylicoccus aureus, Erysipelothrix rhusiopathiae, and Streptococcus suis were determined in vitro. The results showed that three alkaloids lycorine, betonicine and ellipticine, were screened out by Cmap. The MIC results showed that ellipticine had significant inhibitory effect on Escherichia coli, Staphylicoccus aureus, Erysipelothrix rhusiopathiae, and Streptococcus suis. And ellipticine had significant inhibitory effect on hemolytic activity of Staphylococcus aureus and Streptococcus suis. But the antibacterial effect of lycorine and betonicine were not significant.

Key words: lycorine, betonicine, ellipticine, antibacterial activity

CLC Number:

S852.61

LIU Man-li, SHU Fen, ZHANG Ya-ni, FANG Wei, WANG Kai-mei, WU Zhao-yuan. The study of antimicrobial drugs screening using Connectivity Map database[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(24): 101-104.

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