Prokaryotic expression of mouse IL-1β and preliminary study on its differential expression in Brucella infection

doi:10.14088/j.cnki.issn0439-8114.2022.06.021

HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (6): 112-116.doi: 10.14088/j.cnki.issn0439-8114.2022.06.021

• Animal Husbandry & Veterinary Medicine • Previous Articles Next Articles

Prokaryotic expression of mouse IL-1β and preliminary study on its differential expression in Brucella infection

YANG Xin, ZOU De-ying, ZHANG Kai, CHANG Jiang, CHANG Heng-zhen, ZHAN Jun-peng, GUO Xun, LIU Yi-xin, CHENG Meng-yan, HAN Cheng, HU Pan, LU Shi-ying, LI Yan-song, LIU Zeng-shan, REN Hong-lin

Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, China

Received:2021-12-09 Online:2022-03-25 Published:2022-04-18

Abstract

Abstract: To explore the expression of IL-1β during the infection of host cells by Brucella, the prokaryotic expression of IL-1β in mouse was constructed, polyclonal antibodies was obtained by immunization, and its activity was detected by ELISA and Western blot. The 1∶100 （bacterial∶cell） multiplicity of infection was then used to model the infection of RAW264.7 macrophages by Brucella suis S2. Expression of IL-1β mRNA and MyD88 mRNA was detected by RT-PCR; IL-1β protein was analyzed by Western blot. The molecular weight of the recombinant protein was about 31 ku. And the titer of the polyclonal antibody prepared by ELISA was 1∶25 600, with good immunoreactivity and specificity. The expression of IL-1β mRNA and IL-1β protein increased after infection. And the number of intracellular bacteria decreased. The expression of IL-1β in macrophages infected with Brucella suis S2 varied over time and affected the intracellular bacteria。

Key words: Brucella, prokaryotic expression, polyclonal antibody, IL-1β, MyD88

CLC Number:

R392

YANG Xin, ZOU De-ying, ZHANG Kai, CHANG Jiang, CHANG Heng-zhen, ZHAN Jun-peng, GUO Xun, LIU Yi-xin, CHENG Meng-yan, HAN Cheng, HU Pan, LU Shi-ying, LI Yan-song, LIU Zeng-shan, REN Hong-lin. Prokaryotic expression of mouse IL-1β and preliminary study on its differential expression in Brucella infection[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(6): 112-116.

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Prokaryotic expression of mouse IL-1β and preliminary study on its differential expression in Brucella infection

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