Therapeutic effects of shikonin on complete Freund's adjuvant-induced rheumatoid arthritis mouse model

doi:10.14088/j.cnki.issn0439-8114.2023.01.024

Abstract

Abstract: To investigate the effect of shikonin （SHI） on rheumatoid arthritis （RA）, ICR mice were randomly divided into 4 groups, including the normal group and three experimental groups （model group, positive drug group and SHI group）. After 15 days of modeling, the drug treatment losted for 60 days. The positive drug group was given meloxicam 50 mg/（kg·d）, the SHI group was given shikonin 2 mg/（kg·d）, and the normal group and model group were given normal saline. The right ankle joint of the mice was separated, and the degree of synovial inflammation （grade 0-3）, cartilage destruction （grade 0-4） and bone infiltration were evaluated. The thymus and spleen of mice were separated and thymus index and spleen index were calculated. Histopathological evaluation and some serum biochemical indexes （including inflammatory factors and antioxidant factors） were analyzed and determined. The results showed that shikonin could significantly improve the symptoms of joint redness in mice. The levels of nitric oxide （NO）,tumor factor α （TNF-α）,interleukin 1β （IL-1β） and interleukin 6 （IL-6）,in the SHI group were significantly lower than those in the AA model group, the level of malondialdehyde （MDA） in serum as significantly lower than that in the AA model group, and the enzyme activity of superoxide dismutase （SOD） was significantly higher than that in the AA model group. The difference was statistically significaht（P<0.05 or P<0.01）. Shikonin could relieve the inflammatory response of RA by reducing the level of inflammatory factors and improving the antioxidant capacity of the body.

Key words: shikonin, complete freund's adjuvant, rheumatoid arthritis, inflammatory cytokines

CLC Number:

HUANG Jun-xia,XIE You-yin, KONG De-yi, SHAO Li-lin, ZHANG Lei-fang. Therapeutic effects of shikonin on complete Freund's adjuvant-induced rheumatoid arthritis mouse model[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(1): 139-144.

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